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. 1978 Jan;133(1):122–129. doi: 10.1128/jb.133.1.122-129.1978

Chromosome replication in Myxococcus xanthus.

D R Zusman, D M Krotoski, M Cumsky
PMCID: PMC221985  PMID: 412830

Abstract

The rates of DNA synthesis during the cell-division cycle were measured in Myxococcus xanthus growing in three different media permitting a twofold variation in doubling time. In all three media, simple DNA cycles were observed. Synthesis of DNA occurred during 85% of the cell-division cycle, independent of generation time, from 5 to 11 h. Cells were observed to contain one bacterial nucleoid at birth that later divided synchronously midway through the cell cycle. Nucleoid segregation appeared to begin before chromosome replication was completed. The DNA content of exponential-phase bacteria was determined to be about 20 +/- 3 X 10(-9) microgram per cell; newborn bacteria contained about 14 +/- 2 X 10(-9) microgram of DNA per cell. Exponential-phase bacteria showed about a 50% increase in DNA in the presence of chloramphenicol (50 microgram/ml). The number of randomly segregating chromosomes present in exponential-phase bacteria was determined by following the fate of prelabeled DNA during outgrowth in nonradioactive media. The results are consistent with a model in which cells are born with exactly one complete unreplicated chromosome. The molecular weight of such a chromosome is about 8.4 +/- 1.2 X 10(9).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  3. Brown N. L., Parish J. H. Extrachromosomal DNA in chloramphenicol resistant myxococcus strains. J Gen Microbiol. 1976 Mar;93(1):63–68. doi: 10.1099/00221287-93-1-63. [DOI] [PubMed] [Google Scholar]
  4. Campos J. M., Zusman D. R. Regulation of development in Myxococcus xanthus: effect of 3':5'-cyclic AMP, ADP, and nutrition. Proc Natl Acad Sci U S A. 1975 Feb;72(2):518–522. doi: 10.1073/pnas.72.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  6. Cuzin F., Jacob F. Existence chez Escherichia coli K12 d'une unité génétique de transmission formée de différents réplicons. Ann Inst Pasteur (Paris) 1967 May;112(5):529–545. [PubMed] [Google Scholar]
  7. Dworkin M. Biology of the myxobacteria. Annu Rev Microbiol. 1966;20:75–106. doi: 10.1146/annurev.mi.20.100166.000451. [DOI] [PubMed] [Google Scholar]
  8. Filer D., Rosenberg E., Kindler S. H. Aspartokinase of Myxococcus xanthus: "feedback stimulation" by required amino acids. J Bacteriol. 1973 Jul;115(1):23–28. doi: 10.1128/jb.115.1.23-28.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gudas L. J., Pardee A. B. Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli: a comparison of strains B-r, K-12, 15, and 15T- under conditions of slow growth. J Bacteriol. 1974 Mar;117(3):1216–1223. doi: 10.1128/jb.117.3.1216-1223.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HANAWALT P. C., MAALOE O., CUMMINGS D. J., SCHAECHTER M. The normal DNA replication cycle. II. J Mol Biol. 1961 Apr;3:156–165. doi: 10.1016/s0022-2836(61)80042-9. [DOI] [PubMed] [Google Scholar]
  11. Hemphill H. E., Zahler S. A. Nutrition of Myxococcus xanthus FBa and some of its auxotrophic mutants. J Bacteriol. 1968 Mar;95(3):1011–1017. doi: 10.1128/jb.95.3.1011-1017.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hemphill H. E., Zahler S. A. Nutritional induction and suppression of fruiting in Myxococcus xanthus FBa. J Bacteriol. 1968 Mar;95(3):1018–1023. doi: 10.1128/jb.95.3.1018-1023.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kimchi A., Rosenberg E. Linkages between deoxyribonucleic acid synthesis and cell division in Myxococcus xanthus. J Bacteriol. 1976 Oct;128(1):69–79. doi: 10.1128/jb.128.1.69-79.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Klotz L. C., Zimm B. H. Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli. J Mol Biol. 1972 Dec 30;72(3):779–800. doi: 10.1016/0022-2836(72)90191-x. [DOI] [PubMed] [Google Scholar]
  15. Lark K. G. Initiation and control of DNA synthesis. Annu Rev Biochem. 1969;38:569–604. doi: 10.1146/annurev.bi.38.070169.003033. [DOI] [PubMed] [Google Scholar]
  16. NOREN B., RAPER K. B. Antibiotic activity of myxobacteria in relation to their bacteriolytic capacity. J Bacteriol. 1962 Jul;84:157–162. doi: 10.1128/jb.84.1.157-162.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pierucci O., Helmstetter C. E. Chromosome replication, protein synthesis and cell division in Escherichia coli. Fed Proc. 1969 Nov-Dec;28(6):1755–1760. [PubMed] [Google Scholar]
  18. Rosenberg E., Vaks B., Zuckerberg A. Bactericidal action of an antibiotic produced by Myxococcus xanthus. Antimicrob Agents Chemother. 1973 Nov;4(5):507–513. doi: 10.1128/aac.4.5.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sudo S., Dworkin M. Bacteriolytic enzymes produced by Myxococcus xanthus. J Bacteriol. 1972 Apr;110(1):236–245. doi: 10.1128/jb.110.1.236-245.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wireman J. W., Dworkin M. Morphogenesis and developmental interactions in myxobacteria. Science. 1975 Aug 15;189(4202):516–523. doi: 10.1126/science.806967. [DOI] [PubMed] [Google Scholar]
  21. Witkin S. S., Rosenberg E. Induction of morphogenesis by methionine starvation in Myxococcus xanthus: polyamine control. J Bacteriol. 1970 Sep;103(3):641–649. doi: 10.1128/jb.103.3.641-649.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zusman D., Gottlieb P., Rosenberg E. Division cycle of Myxococcus xanthus. 3. Kinetics of cell growth and protein synthesis. J Bacteriol. 1971 Mar;105(3):811–819. doi: 10.1128/jb.105.3.811-819.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zusman D., Rosenberg E. Deoxyribonucleic acid synthesis during microcyst germination in Myxococcus xanthus. J Bacteriol. 1968 Oct;96(4):981–986. doi: 10.1128/jb.96.4.981-986.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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