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. 1978 Jan;133(1):165–171. doi: 10.1128/jb.133.1.165-171.1978

Peptide utilization in Pseudomonas aeruginosa: evidence for membrane-associated peptidase.

R V Miller, J M Becker
PMCID: PMC221990  PMID: 412832

Abstract

A methionine auxotroph of Pseudomonas aeruginosa grew on methionine-containing peptides as a source of the required amino acid. Amino-terminus-blocked peptides would not serve as growth substrates, despite the fact that peptidases active on these blocked peptides were readily detectable in cell extracts. No evidence was found for extracellular enzymes capable of degrading the oligopeptides investigated. The degradative enzymes were not found in the periplasmic space of the cellular envelope. A high proportion of cellular peptidase activity was associated with the particulate (membrane) fraction of the cell lysate.

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Selected References

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  1. Ames B. N., Ames G. F., Young J. D., Tsuchiya D., Lecocq J. Illicit transport: the oligopeptide permease. Proc Natl Acad Sci U S A. 1973 Feb;70(2):456–458. doi: 10.1073/pnas.70.2.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barak Z., Gilvarg C. Peptide transport. Biomembranes. 1975;7:167–218. doi: 10.1007/978-1-4684-7668-2_7. [DOI] [PubMed] [Google Scholar]
  3. Becker J. M., Naider F. Stereospecificity of tripeptide utilization in a methionine auxotroph of Escherichia coli K-12. J Bacteriol. 1974 Oct;120(1):191–196. doi: 10.1128/jb.120.1.191-196.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bekcer J. M., Naider F. Peptide transport in yeast: uptake of radioactive trimethionine in Saccharomyces cerevisiae. Arch Biochem Biophys. 1977 Jan 15;178(1):245–255. doi: 10.1016/0003-9861(77)90189-8. [DOI] [PubMed] [Google Scholar]
  5. Cascieri T., Mallette M. F. Intracellular peptide hydrolysis by Pseudomonas putida and Pseudomonas maltophilia. J Gen Microbiol. 1976 Feb;92(2):296–303. doi: 10.1099/00221287-92-2-296. [DOI] [PubMed] [Google Scholar]
  6. Cascieri T., Mallette M. F. Peptide utilization by Pseudomonas putida and Pseudomonas maltophilia. J Gen Microbiol. 1976 Feb;92(2):283–295. doi: 10.1099/00221287-92-2-283. [DOI] [PubMed] [Google Scholar]
  7. Choules G. L., Gray W. R. Peptidase activity in the membranes of Mycoplasma laidlawii. Biochem Biophys Res Commun. 1971 Nov;45(4):849–855. doi: 10.1016/0006-291x(71)90416-5. [DOI] [PubMed] [Google Scholar]
  8. Fickel T. E., Gilvarg C. Transport of impermeant substances in E. coli by way of oligopeptide permease. Nat New Biol. 1973 Feb 7;241(110):161–163. doi: 10.1038/newbio241161a0. [DOI] [PubMed] [Google Scholar]
  9. Fujita M., Parsons D. S., Wojnarowska F. Oligopeptidases of brush border membranes of rat small intestinal mucosal cells. J Physiol. 1972 Dec;227(2):377–394. doi: 10.1113/jphysiol.1972.sp010038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GILVARG C., KATCHALSKI E. PEPTIDE UTILIZATION IN ESCHERICHIA COLI. J Biol Chem. 1965 Jul;240:3093–3098. [PubMed] [Google Scholar]
  11. Izaki K., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XIV. Purification and properties of two D-alanine carboxypeptidases from Escherichia coli. J Biol Chem. 1968 Jun 10;243(11):3193–3201. [PubMed] [Google Scholar]
  12. Jackson M. B., Becker J. M. Oligopeptide transport in proline peptidase mutants of Salmonella typhimurium. J Biol Chem. 1976 Sep 10;251(17):5300–5309. [PubMed] [Google Scholar]
  13. Johnson G. G., Morris J. M., Berk R. S. The extracellular protease from Pseudomonas aeruginosa exhibiting elastase activity. Can J Microbiol. 1967 Jun;13(6):711–719. doi: 10.1139/m67-093. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lichliter W. D., Naider F., Becker J. M. Basis for the design of anticandidal agents from studies of peptide utilization in Canadida albicans. Antimicrob Agents Chemother. 1976 Sep;10(3):483–490. doi: 10.1128/aac.10.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Liu P. V. Extracellular toxins of Pseudomonas aeruginosa. J Infect Dis. 1974 Nov;130 (Suppl)(0):S94–S99. doi: 10.1093/infdis/130.supplement.s94. [DOI] [PubMed] [Google Scholar]
  17. Naider F., Becker J. M., Katzir-Katchalski E. Utilization of methionine-containing peptides and their derivatives by a methionine-requiring auxotroph of Saccharomyces cerevisiae. J Biol Chem. 1974 Jan 10;249(1):9–20. [PubMed] [Google Scholar]
  18. Naider F., Becker J. M. Multiplicity of oligopeptide transport systems in Escherichia coli. J Bacteriol. 1975 Jun;122(3):1208–1215. doi: 10.1128/jb.122.3.1208-1215.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  20. Payne J. W. Peptides and micro-organisms. Adv Microb Physiol. 1976;13:55–113. doi: 10.1016/s0065-2911(08)60038-7. [DOI] [PubMed] [Google Scholar]
  21. Presslitz J. E., Ray V. A. DD-carboxypeptidase and peptidoglycan transpeptidase from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1975 May;7(5):578–581. doi: 10.1128/aac.7.5.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Prusiner S., Doak C. W., Kirk G. A novel mechanism for group translocation: substrate-product reutilization by gamma-glutamyl transpeptidase in peptide and amino acid transport. J Cell Physiol. 1976 Dec;89(4):853–863. doi: 10.1002/jcp.1040890453. [DOI] [PubMed] [Google Scholar]
  23. Simmonds S., Toye N. O. Peptidases in spheroplasts of Escherichia coli K-12. J Biol Chem. 1966 Aug 25;241(16):3852–3860. [PubMed] [Google Scholar]
  24. Sussman A. J., Gilvarg C. Peptide transport and metabolism in bacteria. Annu Rev Biochem. 1971;40:397–408. doi: 10.1146/annurev.bi.40.070171.002145. [DOI] [PubMed] [Google Scholar]
  25. Ugolev A. M., De Laey P. Membrane digestion. A concept of enzyme hydrolysis on cell membranes. Biochim Biophys Acta. 1973 Sep 10;300(2):105–128. doi: 10.1016/0304-4157(73)90001-4. [DOI] [PubMed] [Google Scholar]
  26. Wolfinbarger L., Jr, Marzluf G. A. Peptide utilization by amino acid auxotrophs of Neurospora crassa. J Bacteriol. 1974 Aug;119(2):371–378. doi: 10.1128/jb.119.2.371-378.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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