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. 1978 Jan;133(1):231–239. doi: 10.1128/jb.133.1.231-239.1978

Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius.

C L Wittenberger, A J Beaman, L N Lee
PMCID: PMC221999  PMID: 618839

Abstract

Streptococcus salivarius (ATCC 25975) produced very low or nondetectable amounts of the extracellular enzyme glucosyltransferase (GTase) when grown in a chemically defined medium. The addition of Tween 80 to this medium resulted in the production of markedly enhanced levels of the enzyme. Oleic acid, the methyl ester of oleic acid, and sucrose each could not substitute for Tween 80 in this regard. The surfactant had no direct activating effect on performed enzyme activity. Tween 80 also stimulated the production of GTase by concentrated cells suspended in defined medium during a time when no measurable growth occurred. Under these conditions, the stimulatory effect of Tween 80 was blocked by chloramphenicol. It was further found that the surfactant dramatically stimulated the differential rate of GTase synthesis. These and other data strongly suggest that Tween 80 stimulates the production of extracellular GTase by acting either directly or indirectly at the level of enzyme synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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