Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Jan;133(1):298–305. doi: 10.1128/jb.133.1.298-305.1978

Temperature-sensitive nature of the rodB maturation in Bacillus subtilis.

H J Rogers, P F Thurman
PMCID: PMC222008  PMID: 412835

Abstract

The Mg2+ requirement of a morphological mutant of Bacillus subtlis, rodB strain 104 was highly temperature sensitive in the presence of halide or nitrate anions. Likewise the morphological change from rod shapes to spheres was dependent upon temperature, the same anions, and the Mg2+ concentration. The three factors interacted. Other rodB mutants behaved similarly. If the rodB strain 104 in its rod form was treated at high temperatures in the absence of either protein or peptidoglycan synthesis and restored to lower temperatures with the syntheses restarted, a partial temporary change toward cocci occurred. In the absence of halides or in the presence of Cl- but not Br-, the cells increased in volume when they changed from rods to cocci.

Full text

PDF
298

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown W. C., Wilson C. R., Lukehart S., Young F. E., Shiflett M. A. Analysis of autolysins in temperature-sensitive morphological mutants of Bacillus subtilis. J Bacteriol. 1976 Jan;125(1):166–173. doi: 10.1128/jb.125.1.166-173.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forsberg C. W., Rogers H. J. Characterization of Bacillus licheniformis 6346 mutants which have altered lytic enzyme activities. J Bacteriol. 1974 May;118(2):358–368. doi: 10.1128/jb.118.2.358-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forsberg C. W., Wyrick P. B., Ward J. B., Rogers H. J. Effect of phosphate limitation on the morphology and wall composition of Bacillus licheniformis and its phosphoglucomutase-deficient mutants. J Bacteriol. 1973 Feb;113(2):969–984. doi: 10.1128/jb.113.2.969-984.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hughes R. C., Tanner P. J., Stokes E. Cell-wall thickening in Bacillus subtilis. Comparison of thickened and normal walls. Biochem J. 1970 Nov;120(1):159–170. doi: 10.1042/bj1200159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kahane I., Ne'eman Z., Razin S. Divalent cations in native and reaggregated mycoplasma membranes. J Bacteriol. 1973 Feb;113(2):666–671. doi: 10.1128/jb.113.2.666-671.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karamata D., McConnell M., Rogers H. J. Mapping of rod mutants of Bacillus subtilis. J Bacteriol. 1972 Jul;111(1):73–79. doi: 10.1128/jb.111.1.73-79.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lusk J. E., Kennedy E. P. Altered phospholipid metabolism in a sodium-sensitive mutant of Escherichia coli. J Bacteriol. 1972 Mar;109(3):1034–1046. doi: 10.1128/jb.109.3.1034-1046.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lusk J. E., Williams R. J., Kennedy E. P. Magnesium and the growth of Escherichia coli. J Biol Chem. 1968 May 25;243(10):2618–2624. [PubMed] [Google Scholar]
  10. MANDELSTAM J., ROGERS H. J. Chloramphenicol-resistant incorporation of amino-acids into Staphylococci and cell-wall synthesis. Nature. 1958 Apr 5;181(4614):956–957. doi: 10.1038/181956a0. [DOI] [PubMed] [Google Scholar]
  11. MITCHELL P. Spectrophotometric estimation of nucleic acid in bacterial suspensions. J Gen Microbiol. 1950 Sep;4(3):399–409. doi: 10.1099/00221287-4-3-399. [DOI] [PubMed] [Google Scholar]
  12. Meers J. L., Tempest D. W. The influence of growth-limiting substrate and medium NaCl concentration on the synthesis of magnesium-binding sites in the walls of Bacillus subtilis var. niger. J Gen Microbiol. 1970 Nov;63(3):325–331. doi: 10.1099/00221287-63-3-325. [DOI] [PubMed] [Google Scholar]
  13. Park M. H., Wong B. B., Lusk J. E. Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology. J Bacteriol. 1976 Jun;126(3):1096–1103. doi: 10.1128/jb.126.3.1096-1103.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Razin S. Physiology of mycoplasmas. Adv Microb Physiol. 1973;10:1–80. doi: 10.1016/s0065-2911(08)60086-7. [DOI] [PubMed] [Google Scholar]
  15. Reaveley D. A., Rogers H. J. Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346. Biochem J. 1969 Jun;113(1):67–79. doi: 10.1042/bj1130067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers H. J., McConnell M., Burdett I. D. Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology. Nature. 1968 Jul 20;219(5151):285–288. doi: 10.1038/219285a0. [DOI] [PubMed] [Google Scholar]
  17. Rogers H. J., McConnell M., Burdett I. D. The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division. J Gen Microbiol. 1970 May;61(2):155–171. doi: 10.1099/00221287-61-2-155. [DOI] [PubMed] [Google Scholar]
  18. Rogers H. J., McConnell M. The role of L-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168. J Gen Microbiol. 1970 May;61(2):173–181. doi: 10.1099/00221287-61-2-173. [DOI] [PubMed] [Google Scholar]
  19. Rogers H. J., Thurman P. F., Buxton R. S. Magnesium and anion requirements of rodB mutants of Bacillus subtilis. J Bacteriol. 1976 Feb;125(2):556–564. doi: 10.1128/jb.125.2.556-564.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers H. J., Thurman P. F., Taylor C., Reeve J. N. Mucopeptide synthesis by rod mutants of Bacillus subtilis. J Gen Microbiol. 1974 Dec;85(2):335–349. doi: 10.1099/00221287-85-2-335. [DOI] [PubMed] [Google Scholar]
  21. Tempest D. W., Dicks J. W., Meers J. L. Magnesium-limited growth of Bacillus subtilis, in pure and mixed cultures, in a chemostat. J Gen Microbiol. 1967 Oct;49(1):139–147. doi: 10.1099/00221287-49-1-139. [DOI] [PubMed] [Google Scholar]
  22. Willecke K., Gries E. M., Oehr P. Coupled transport of citrate and magnesium in Bacillus subtilis. J Biol Chem. 1973 Feb 10;248(3):807–814. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES