Abstract
Cells of a thymineless mutant of Escherichia coli B/r are shown to change their shape when the concentration of thymine in the growth medium is reduced. Electron micrographs of whole cells and isolated sacculi were used to make quantitative measurements of the changes in cell length and width which occur as a result of such a change in thymine concentration. The results showed that there is an increase in cell volume, which is due to an increase in cell width accompanied by a decrease in cell length. These changes were compared with the predictions of models which assume that cell shape is influenced by the chromosome replication cycle.
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- Beacham I. R., Beacham K., Zaritsky A., Pritchard R. H. Intracellular thymidine triphosphate concentrations in wild type and in thymine requiring mutants of Escherichia coli 15 and K12. J Mol Biol. 1971 Aug 28;60(1):75–86. doi: 10.1016/0022-2836(71)90448-7. [DOI] [PubMed] [Google Scholar]
- Bleecken S. Duplication of the bacterial cell and its initiation. J Theor Biol. 1969 Oct;25(1):137–158. doi: 10.1016/s0022-5193(69)80021-4. [DOI] [PubMed] [Google Scholar]
- CAMPBELL A. Synchronization of cell division. Bacteriol Rev. 1957 Dec;21(4):263–272. doi: 10.1128/br.21.4.263-272.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
- Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
- Donachie W. D., Begg K. J., Vicente M. Cell length, cell growth and cell division. Nature. 1976 Nov 25;264(5584):328–333. doi: 10.1038/264328a0. [DOI] [PubMed] [Google Scholar]
- Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
- Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
- Hosono R., Hosono H., Kuno S. Effects of growth conditions on thymidine nucleotide pools in Escherichia coli. J Biochem. 1975 Jul;78(1):123–129. [PubMed] [Google Scholar]
- Hosono R., Kuno S. An inhibitory effect of thymidine on its own conversion to nucleotide in Escherichia coli. Eur J Biochem. 1975 Sep 1;57(1):177–179. doi: 10.1111/j.1432-1033.1975.tb02288.x. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., ARBER W. Electron microscopical studies of phage multiplication. I. A method for quantitative analysis of particle suspensions. Virology. 1957 Apr;3(2):245–255. doi: 10.1016/0042-6822(57)90091-0. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karamata D. Polystyrene spheres in electron microscopy. J Ultrastruct Res. 1971 May;35(3):201–209. doi: 10.1016/s0022-5320(71)80152-1. [DOI] [PubMed] [Google Scholar]
- Marr A. G., Harvey R. J., Trentini W. C. Growth and division of Escherichia coli. J Bacteriol. 1966 Jun;91(6):2388–2389. doi: 10.1128/jb.91.6.2388-2389.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meacock P. A., Pritchard R. H. Relationship between chromosome replication and cell division in a thymineless mutant of Escherichia coli B/r. J Bacteriol. 1975 Jun;122(3):931–942. doi: 10.1128/jb.122.3.931-942.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OKAZAKI T., STROMINGER J. L., OKAZAKI R. THYMIDINE DIPHOSPHATE-L-RHAMNOSE METABOLISM IN SMOOTH AND ROUGH STRAINS OF ESCHERICHIA COLI AND SALMONELLA WESLACO. J Bacteriol. 1963 Jul;86:118–124. doi: 10.1128/jb.86.1.118-124.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Previc E. P. Biochemical determination of bacterial morphology and the geometry of cell division. J Theor Biol. 1970 Jun;27(3):471–497. doi: 10.1016/s0022-5193(70)80010-8. [DOI] [PubMed] [Google Scholar]
- Pritchard R. H. Review lecture on the growth and form of a bacterial cell. Philos Trans R Soc Lond B Biol Sci. 1974 Feb 21;267(886):303–336. doi: 10.1098/rstb.1974.0003. [DOI] [PubMed] [Google Scholar]
- Pritchard R. H., Zaritsky A. Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli. Nature. 1970 Apr 11;226(5241):126–131. doi: 10.1038/226126a0. [DOI] [PubMed] [Google Scholar]
- Ryter A., Hirota Y., Schwarz U. Process of cellular division in Escherichia coli growth pattern of E. coli murein. J Mol Biol. 1973 Jun 25;78(1):185–195. doi: 10.1016/0022-2836(73)90437-3. [DOI] [PubMed] [Google Scholar]
- SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
- Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
- Woldringh C. L. Morphological analysis of nuclear separation and cell division during the life cycle of Escherichia coli. J Bacteriol. 1976 Jan;125(1):248–257. doi: 10.1128/jb.125.1.248-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaritsky A. On dimensional determination of rod-shaped bacteria. J Theor Biol. 1975 Oct;54(2):243–248. doi: 10.1016/s0022-5193(75)80129-9. [DOI] [PubMed] [Google Scholar]
- Zaritsky A., Pritchard R. H. Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. J Bacteriol. 1973 May;114(2):824–837. doi: 10.1128/jb.114.2.824-837.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]