Abstract
Plasmid Mor174 has a molecular weight of 3.6 X 10(6) and a buoyant density of 1.6994 g/cm3. The covalently closed circular form has a sedimentation coefficient of 22S. These are 30 to 40 plasmid copies per genome equivalent, but growth in chloramphenicol results in amplification of the copy number to 600. In Proteus morganii 174, Mor174 coexists with a cryptic plasmid of molecular weight 15.8 X 10(6) and a buoyant density of 1.7170 g/cm3.
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Selected References
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- Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
- Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy K. G. Colicinogeny and related phenomena. Bacteriol Rev. 1975 Dec;39(4):464–515. doi: 10.1128/br.39.4.464-515.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sidikaro J., Masayasu N. In vitro synthesis of the E3 immunity protein directed by Col E3 plasmid deoxyribonucleic acid. J Biol Chem. 1975 Feb 10;250(3):1123–1131. [PubMed] [Google Scholar]
- Veltkamp E., Barendsen W., Nijkamp H. J. Influence of protein and ribonucleic acid synthesis on the replication of the bacteriocinogenic factor Clo DF13 in Escherichia coli cells and minicells. J Bacteriol. 1974 Apr;118(1):165–174. doi: 10.1128/jb.118.1.165-174.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N. S. The elimination of Flac+ from Escherichia coli by mutagenic agents. Biochem Biophys Res Commun. 1967 Apr 7;27(1):112–117. doi: 10.1016/s0006-291x(67)80048-2. [DOI] [PubMed] [Google Scholar]
- Williams J. A., Krizsanovich-Williams K. Mode of action of morganocin 174. Antimicrob Agents Chemother. 1977 Sep;12(3):395–400. doi: 10.1128/aac.12.3.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams J. A. Mobilization of morganocin 174 plasmid and kinetics of morganocin production in Proteus and Escherichia coli hosts. Antimicrob Agents Chemother. 1977 Mar;11(3):514–520. doi: 10.1128/aac.11.3.514. [DOI] [PMC free article] [PubMed] [Google Scholar]