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. 1978 Feb;133(2):492–498. doi: 10.1128/jb.133.2.492-498.1978

Physiology of lysine permeases in Saccharomycopsis lipolytica.

J M Beckerich, H Heslot
PMCID: PMC222050  PMID: 627530

Abstract

Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km was 1.91 +/- 0.23 X 10(-5) M. It proved highly specific, the only potent competitive inhibitors being (i) arginine and its analogs L-canavanine and L-ornithine, and (ii) the lysine analogs L-5 aminoethylcysteine and L-4,5-transdehydrolysine. It is suggested that the high-affinity lysine permease is common to L-lysine, L-ornithine, and L-arginine. The other amino acids tested behaved as noncompetitive inhibitors. The variation of uptake during a growth cycle was studied on ammonia-rich, ammonia-poor, and ammonia-free media. In each case, the uptake exhibited a peak in the early exponential growth phase. No new permease activity was detected during the lag phase or the stationary phase. Ammonia ions competitively inhibited the uptake and also decreased the Vmax value.

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Selected References

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  1. Benko P. V., Wood T. C., Segel I. H. Multiplicity and regulation of amino acid transport in Penicillium chrysogenum. Arch Biochem Biophys. 1969 Feb;129(2):498–508. doi: 10.1016/0003-9861(69)90207-0. [DOI] [PubMed] [Google Scholar]
  2. Boller T., Dürr M., Wiemken A. Characterization of a specific transport system for arginine in isolated yeast vacuoles. Eur J Biochem. 1975 May;54(1):81–91. doi: 10.1111/j.1432-1033.1975.tb04116.x. [DOI] [PubMed] [Google Scholar]
  3. Christensen H. N., Liang M. Transport of diamino acids into the Ehrlich cell. J Biol Chem. 1966 Dec 10;241(23):5542–5551. [PubMed] [Google Scholar]
  4. Gaillardin C. M., Poirier L., Heslot H. A kinetic study of homocitrate synthetase activity in the yeast Saccharomycopsis lipolytica. Biochim Biophys Acta. 1976 Feb 13;422(2):390–406. doi: 10.1016/0005-2744(76)90150-9. [DOI] [PubMed] [Google Scholar]
  5. Gaillardin C. M., Sylvestre G., Heslot H. Studies on an unstable phenotype induced by UV irradiation: the lysine excreting (lex(-)) phenotype of the yeast Saccharomycosis lipolytica. Arch Microbiol. 1975 Jun 20;104(1):89–94. doi: 10.1007/BF00447305. [DOI] [PubMed] [Google Scholar]
  6. Gaillardin C., Fournier P., Sylvestre G., Heslot H. Mutants of Saccharomycopsis lipolytica defective in lysine catabolism. J Bacteriol. 1976 Jan;125(1):48–57. doi: 10.1128/jb.125.1.48-57.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gits J. J., Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. doi: 10.1016/0005-2736(67)90040-5. [DOI] [PubMed] [Google Scholar]
  8. Grenson M., Crabeel M., Wiame J. M., Béchet J. Inhibition of protein synthesis and simulation of permease turnover in yeast. Biochem Biophys Res Commun. 1968 Feb 26;30(4):414–419. doi: 10.1016/0006-291x(68)90760-2. [DOI] [PubMed] [Google Scholar]
  9. Grenson M., Hou C. Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of saccharomyces cerevisiae. Biochem Biophys Res Commun. 1972 Aug 21;48(4):749–756. doi: 10.1016/0006-291x(72)90670-5. [DOI] [PubMed] [Google Scholar]
  10. Grenson M., Hou C., Crabeel M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J Bacteriol. 1970 Sep;103(3):770–777. doi: 10.1128/jb.103.3.770-777.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grenson M., Mousset M., Wiame J. M., Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. doi: 10.1016/0304-4165(66)90387-4. [DOI] [PubMed] [Google Scholar]
  12. Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):339–346. doi: 10.1016/0304-4165(66)90388-6. [DOI] [PubMed] [Google Scholar]
  13. Hunter D. R., Segel I. H. Acidic and basic amino acid transport systems of Penicillium chrysogenum. Arch Biochem Biophys. 1971 May;144(1):168–183. doi: 10.1016/0003-9861(71)90466-8. [DOI] [PubMed] [Google Scholar]
  14. Hunter D. R., Segel I. H. Control of the general amino acid permease of Penicillium chrysogenum by transinhibition and turnover. Arch Biochem Biophys. 1973 Jan;154(1):387–399. doi: 10.1016/0003-9861(73)90071-4. [DOI] [PubMed] [Google Scholar]
  15. Kepes A. Trois classes de systèmes de transport chez les bactéries. Biochimie. 1973;55(6):693–702. [PubMed] [Google Scholar]
  16. Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
  17. Piotrowska M., Stepień P. P., Bartnik E., Zakrzewska E. Basic and neutral amino acid transport in Aspergillus nidulans. J Gen Microbiol. 1976 Jan;92(1):89–96. doi: 10.1099/00221287-92-1-89. [DOI] [PubMed] [Google Scholar]
  18. Quay S., Christensen H. N. Basis of transport discrimination of arginine from other basic amino acids in Salmonella typhimurium. J Biol Chem. 1974 Nov 10;249(21):7011–7017. [PubMed] [Google Scholar]
  19. Robinson J. H., Anthony C., Drabble W. T. Regulation of the acidic amino-acid permease of Aspergillus nidulans. J Gen Microbiol. 1973 Nov;79(1):65–80. doi: 10.1099/00221287-79-1-65. [DOI] [PubMed] [Google Scholar]
  20. Robinson J. H., Anthony C., Drabble W. T. The acidic amino-acid permease of Aspergillus nidulans. J Gen Microbiol. 1973 Nov;79(1):53–63. doi: 10.1099/00221287-79-1-53. [DOI] [PubMed] [Google Scholar]
  21. Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
  22. Subramanian K. N., Weiss R. L., Davis R. H. Use of external, biosynthetic, and organellar arginine by Neurospora. J Bacteriol. 1973 Jul;115(1):284–290. doi: 10.1128/jb.115.1.284-290.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tisdale J. H., DeBusk A. G. Developmental regulation of amino acid transport in Neurospora crassa. J Bacteriol. 1970 Nov;104(2):689–697. doi: 10.1128/jb.104.2.689-697.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wilson O. H., Holden J. T. Arginine transport and metabolism in osmotically shocked and unshocked cells of Escherichia coli W. J Biol Chem. 1969 May 25;244(10):2737–2742. [PubMed] [Google Scholar]

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