Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Feb;133(2):667–670. doi: 10.1128/jb.133.2.667-670.1978

Mapping of a genetic locus that affects glycerol 3-phosphate transport in Bacillus subtilis.

V Lindgren
PMCID: PMC222073  PMID: 415047

Abstract

Two types of fosfomycin-resistant mutants of Bacillus subtilis were isolated. Mutants of the first type (GlpT mutants) were resistant to at least 200 microgram of fosfomycin per ml and failed to take up exogenous glycerol 3-phosphate. Mutants of the second type were resistant to lower concentrations of fosfomycin and transported glycerol-3-phosphate as efficiently as wild-type bacteria. The glpT mutations, but not the mutations in the second type of fosfomycin-resistant mutants, map in the cysA-aroI region of the B. subtilis chromosome.

Full text

PDF
667

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berman M., Lin E. C. Glycerol-specific revertants of a phosphoenolpyruvate phosphotransferase mutant: suppression by the desensitization of glycerol kinase to feedback inhibition. J Bacteriol. 1971 Jan;105(1):113–120. doi: 10.1128/jb.105.1.113-120.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bron S., Venema G. Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives. Mutat Res. 1972 May;15(1):1–10. doi: 10.1016/0027-5107(72)90086-3. [DOI] [PubMed] [Google Scholar]
  4. Cordaro J. C., Melton T., Stratis J. P., Atagün M., Gladding C., Hartman P. E., Roseman S. Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium. J Bacteriol. 1976 Dec;128(3):785–793. doi: 10.1128/jb.128.3.785-793.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  6. Dul M. J., Young F. E. Genetic mapping of a mutant defective in D,L-alanine racemase in Bacillus subtilis 168. J Bacteriol. 1973 Sep;115(3):1212–1214. doi: 10.1128/jb.115.3.1212-1214.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
  8. Gay P., Cordier P., Marquet M., Delobbe A. Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations. Mol Gen Genet. 1973 Mar 19;121(4):355–368. doi: 10.1007/BF00433234. [DOI] [PubMed] [Google Scholar]
  9. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  10. Harford N., Sueoka N. Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J Mol Biol. 1970 Jul 28;51(2):267–286. doi: 10.1016/0022-2836(70)90142-7. [DOI] [PubMed] [Google Scholar]
  11. Hoch J. A. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis. J Bacteriol. 1971 Mar;105(3):896–901. doi: 10.1128/jb.105.3.896-901.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kadner R. J., Winkler H. H. Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli. J Bacteriol. 1973 Feb;113(2):895–900. doi: 10.1128/jb.113.2.895-900.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kahan F. M., Kahan J. S., Cassidy P. J., Kropp H. The mechanism of action of fosfomycin (phosphonomycin). Ann N Y Acad Sci. 1974 May 10;235(0):364–386. doi: 10.1111/j.1749-6632.1974.tb43277.x. [DOI] [PubMed] [Google Scholar]
  14. Lepesant-Kejzlarová J., Lepesant J. A., Walle J., Billault A., Dedonder R. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome. J Bacteriol. 1975 Mar;121(3):823–834. doi: 10.1128/jb.121.3.823-834.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lindgren V., Rutberg L. Genetic control of the glp system in Bacillus subtilis. J Bacteriol. 1976 Sep;127(3):1047–1057. doi: 10.1128/jb.127.3.1047-1057.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindgren V., Rutberg L. Glycerol metabolism in Bacillus subtilis: gene-enzyme relationships. J Bacteriol. 1974 Aug;119(2):431–442. doi: 10.1128/jb.119.2.431-442.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miki T., Minami Z., Ikeda Y. The genetics of alkaline phosphatase formation in Bacillus subtilis. Genetics. 1965 Nov;52(5):1093–1100. doi: 10.1093/genetics/52.5.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mindich L. Pathway for oxidative dissimilation of glycerol in Bacillur subtilis. J Bacteriol. 1968 Aug;96(2):565–566. doi: 10.1128/jb.96.2.565-566.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Saheb S. A. Etude de deux mutants du métabolisme du glycérol chez Bacillus subtilis. Can J Microbiol. 1972 Aug;18(8):1315–1325. [PubMed] [Google Scholar]
  20. Saier M. H., Roseman S. Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1972 Feb 10;247(3):972–975. [PubMed] [Google Scholar]
  21. Venkateswaran P. S., Wu H. C. Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):935–944. doi: 10.1128/jb.110.3.935-944.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Winkler H. H. Distribution of an inducible hexose-phosphate transport system among various bacteria. J Bacteriol. 1973 Nov;116(2):1079–1081. doi: 10.1128/jb.116.2.1079-1081.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES