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. 1978 Feb;133(2):822–829. doi: 10.1128/jb.133.2.822-829.1978

Cellular autolytic activity in synchronized populations of Streptococcus faecium.

R P Hinks, L Daneo-Moore, G D Shockman
PMCID: PMC222094  PMID: 415050

Abstract

The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studies of cells containing overlapping chromosome cycles and possible artifacts generated by induction techniques, the autolytic capacities of slowly dividing populations (TD=60 to 110 min) synchronized by selection only were examined. Although the overall level of cellular autolytic capacity was observed to decline with decreasing growth rate, sharp, periodic fluctuations in cellular autolytic capacity were seen during synchronous growth at all growth rates examined. On the basis of similar patterns of cyclic fluctuations in autolytic capacity of cultures synchronized by (i) selection, (ii) amino acid starvation followed by size selection, and (iii) amino acid starvation followed by inhibition of DNA synthesis, a link of such fluctuations with the cell division cycle has been postulated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck B. D., Park J. T. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. J Bacteriol. 1976 Jun;126(3):1250–1260. doi: 10.1128/jb.126.3.1250-1260.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boothby D., Daneo-Moore L., Higgins M. L., Coyette J., Shockman G. D. Turnover of bacterial cell wall peptidoglycans. J Biol Chem. 1973 Mar 25;248(6):2161–2169. [PubMed] [Google Scholar]
  3. Cleveland R. F., Daneo-Moore L., Wicken A. J., Shockman G. D. Effect of lipoteichoic acid and lipids on lysis of intact cells of Streptococcus faecalis. J Bacteriol. 1976 Sep;127(3):1582–1584. doi: 10.1128/jb.127.3.1582-1584.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cleveland R. F., Holtje J. V., Wicken A. J., Tomasz A., Daneo-Moore L., Shockman G. D. Inhibition of bacterial wall lysins by lipoteichoic acids and related compounds. Biochem Biophys Res Commun. 1975 Dec 1;67(3):1128–1135. doi: 10.1016/0006-291x(75)90791-3. [DOI] [PubMed] [Google Scholar]
  5. Cleveland R. F., Wicken A. J., Daneo-Moore L., Shockman G. D. Inhibition of wall autolysis in Streptococcus faecalis by lipoteichoic acid and lipids. J Bacteriol. 1976 Apr;126(1):192–197. doi: 10.1128/jb.126.1.192-197.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hakenbeck R., Messer W. Activity of murein hydrolases and membrane synthesis in synchronized Escherichia coli B/r. Ann Microbiol (Paris) 1974 Sep;125 B(2):163–166. [PubMed] [Google Scholar]
  7. Hakenbeck R., Messer W. Oscillations in the synthesis of cell wall components in synchronized cultures of Escherichia coli. J Bacteriol. 1977 Mar;129(3):1234–1238. doi: 10.1128/jb.129.3.1234-1238.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Higgins M. L., Daneo-Moore L., Boothby D., Shockman G. D. Effect of inhibition of deoxyribonucleic acid and protein synthesis on the direction of cell wall growth in Streptococcus faecalis. J Bacteriol. 1974 May;118(2):681–692. doi: 10.1128/jb.118.2.681-692.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Higgins M. L., Pooley H. M., Shockman G. D. Reinitiation of cell wall growth after threonine starvation of Streptococcus faecalis. J Bacteriol. 1971 Mar;105(3):1175–1183. doi: 10.1128/jb.105.3.1175-1183.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Higgins M. L., Pooley H. M., Shockman G. D. Site of initiation of cellular autolysis in Streptococcus faecalis as seen by electron microscopy. J Bacteriol. 1970 Aug;103(2):504–512. doi: 10.1128/jb.103.2.504-512.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Higgins M. L., Shockman G. D. Procaryotic cell division with respect to wall and membranes. CRC Crit Rev Microbiol. 1971 May;1(1):29–72. doi: 10.3109/10408417109104477. [DOI] [PubMed] [Google Scholar]
  12. Higgins M. L., Shockman G. D. Study of cycle of cell wall assembly in Streptococcus faecalis by three-dimensional reconstructions of thin sections of cells. J Bacteriol. 1976 Sep;127(3):1346–1358. doi: 10.1128/jb.127.3.1346-1358.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoffmann B., Messer W., Schwarz U. Regulation of polar cap formation in the life cycle of Escherichia coli. J Supramol Struct. 1972;1(1):29–37. doi: 10.1002/jss.400010105. [DOI] [PubMed] [Google Scholar]
  14. Joseph R., Shockman G. D. Autolytic formation of protoplasts (autoplasts) of Streptococcus faecalis; location of active and latent autolysin. J Bacteriol. 1976 Sep;127(3):1482–1493. doi: 10.1128/jb.127.3.1482-1493.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MITCHELL P., MOYLE J. Autolytic release and osmotic properties of protoplasts from Staphylococcus aureus. J Gen Microbiol. 1957 Feb;16(1):184–194. doi: 10.1099/00221287-16-1-184. [DOI] [PubMed] [Google Scholar]
  16. Mirelman D., Yashouv-Gan Y., Schwarz U. Peptidoglycan biosynthesis in a thermosensitive division mutant of Escherichia coli. Biochemistry. 1976 May 4;15(9):1781–1790. doi: 10.1021/bi00654a001. [DOI] [PubMed] [Google Scholar]
  17. Mirelman D., Yashouv-Gan Y., Schwarz U. Regulation of murein biosynthesis and septum formation in filamentous cells of Escherichia coli PAT 84. J Bacteriol. 1977 Mar;129(3):1593–1600. doi: 10.1128/jb.129.3.1593-1600.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pooley H. M., Shockman G. D. Relationship between the location of autolysin, cell wall synthesis, and the development of resistance to cellular autolysis in Streptococcus faecalis after inhibition of protein synthesis. J Bacteriol. 1970 Aug;103(2):457–466. doi: 10.1128/jb.103.2.457-466.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sayare M., Daneo-Moore L., Shockman G. D. Influence of macromolecular biosynthesis on cellular autolysis in Streptococcus faecalis. J Bacteriol. 1972 Oct;112(1):337–344. doi: 10.1128/jb.112.1.337-344.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shockman G. D., Daneo-Moore L., Higgins M. L. Problems of cell wall and membrane growth, enlargement, and division. Ann N Y Acad Sci. 1974 May 10;235(0):161–197. doi: 10.1111/j.1749-6632.1974.tb43265.x. [DOI] [PubMed] [Google Scholar]
  21. Shockman G. D., Pooley H. M., Thompson J. S. Autolytic enzyme system of Streptococcus faecalis. 3. Localization of the autolysin at the sites of cell wall synthesis. J Bacteriol. 1967 Nov;94(5):1525–1530. doi: 10.1128/jb.94.5.1525-1530.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shockman G. D. Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. Bacteriol Rev. 1965 Sep;29(3):345–358. doi: 10.1128/br.29.3.345-358.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shockman G. D., Thompson J. S., Conover M. J. The autolytic enzyme system of Streptococcus faecalis. II. Partial characterization of the autolysin and its substrate. Biochemistry. 1967 Apr;6(4):1054–1065. doi: 10.1021/bi00856a014. [DOI] [PubMed] [Google Scholar]
  24. TOENNIES G., ISZARD L., ROGERS N. B., SHOCKMAN G. D. Cell multiplication studied with an electronic particle counter. J Bacteriol. 1961 Dec;82:857–866. doi: 10.1128/jb.82.6.857-866.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WEIDEL W., PELZER H. BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS. Adv Enzymol Relat Areas Mol Biol. 1964;26:193–232. doi: 10.1002/9780470122716.ch5. [DOI] [PubMed] [Google Scholar]
  26. Wolf-Watz H., Normark S. Evidence for a role of N-acetylmuramyl-L-alanine amidase in septum separation in Escherichia coli. J Bacteriol. 1976 Nov;128(2):580–586. doi: 10.1128/jb.128.2.580-586.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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