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. 1978 Feb;133(2):844–851. doi: 10.1128/jb.133.2.844-851.1978

Deg phenotype of Escherichia coli lon mutants.

S Gottesman, D Zipser
PMCID: PMC222096  PMID: 146704

Abstract

Deg. one of the Escherichia coli systems for degrading abnormal polypeptides (e.g., nonsense fragments), is also involved in the degradation of some classes of missense proteins. Both missense proteins of beta-galactosidase and temperature-sensitive phage products appear to be degraded by the Deg system. Mutations in the Deg system are indistinguishable from mutations classically called lon or capR; all map near proC, all are mucoid, defective in protein degradation, sensitive to radiomimetic agents, and defective in P1 lysogenization. All are able to propagate temperature-sensitive phage better than lon+ parental strains. Mutations that suppress the radiation sensitivity of these strains (sul) also suppress the P1 lysogenization defect, but do not affect mucoidy or the degradation defect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apte B. N., Rhodes H., Zipser D. Mutation blocking the specific degradation of reinitiation polypeptides in E. coli. Nature. 1975 Sep 25;257(5524):329–331. doi: 10.1038/257329a0. [DOI] [PubMed] [Google Scholar]
  2. Bukhari A. I., Zipser D. Mutants of Escherichia coli with a defect in the degradation of nonsense fragments. Nat New Biol. 1973 Jun 20;243(129):238–241. doi: 10.1038/newbio243238a0. [DOI] [PubMed] [Google Scholar]
  3. Donch J., Greenberg J. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol Gen Genet. 1968;103(2):105–115. doi: 10.1007/BF00427138. [DOI] [PubMed] [Google Scholar]
  4. Gayda R. C., Yamamoto L. T., Markovitz A. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. J Bacteriol. 1976 Sep;127(3):1208–1216. doi: 10.1128/jb.127.3.1208-1216.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. George J., Castellazzi M., Buttin G. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol Gen Genet. 1975 Oct 22;140(4):309–332. [PubMed] [Google Scholar]
  6. Goldberg A. L., Dice J. F. Intracellular protein degradation in mammalian and bacterial cells. Annu Rev Biochem. 1974;43(0):835–869. doi: 10.1146/annurev.bi.43.070174.004155. [DOI] [PubMed] [Google Scholar]
  7. Goldberg A. L., St John A. C. Intracellular protein degradation in mammalian and bacterial cells: Part 2. Annu Rev Biochem. 1976;45:747–803. doi: 10.1146/annurev.bi.45.070176.003531. [DOI] [PubMed] [Google Scholar]
  8. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hua S. S., Markovitz A. Multiple regulator gene control of the galactose operon in Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):1089–1099. doi: 10.1128/jb.110.3.1089-1099.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson B. F., Greenberg J. Mapping of sul, the suppressor of lon in Escherichia coli. J Bacteriol. 1975 May;122(2):570–574. doi: 10.1128/jb.122.2.570-574.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lin S., Zabin I. Beta-galactosidase. Rates of synthesis and degradation of incomplete chains. J Biol Chem. 1972 Apr 10;247(7):2205–2211. [PubMed] [Google Scholar]
  12. Mackie G., Wilson D. B. Regulation of the gal operon of Escherichia coli by the capR gene. J Biol Chem. 1972 May 25;247(10):2973–2978. [PubMed] [Google Scholar]
  13. Markovitz A., Rosenbaum N. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1084–1091. doi: 10.1073/pnas.54.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller C. G., Zipser D. Degradation of Escherichia coli beta-galactosidase fragments in protease-deficient mutants of Salmonella typhimurium. J Bacteriol. 1977 Apr;130(1):347–353. doi: 10.1128/jb.130.1.347-353.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  16. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  17. Shineberg B., Zipser D. The ion gene and degradation of beta-galactosidase nonsense fragments. J Bacteriol. 1973 Dec;116(3):1469–1471. doi: 10.1128/jb.116.3.1469-1471.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takano T. Bacterial mutants defective in plasmid formation: requirement for the lon + allele. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1469–1473. doi: 10.1073/pnas.68.7.1469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Truitt C. L., Haldenwang W. G., Walker J. R. Interaction of host and viral regulatory mechanisms: effect of the ion cell division defect on regulation of repression by bacteriophage lambda. J Mol Biol. 1976 Aug 5;105(2):231–244. doi: 10.1016/0022-2836(76)90109-1. [DOI] [PubMed] [Google Scholar]
  20. Uretz R. B., Markovitz A. Dominance of ultraviolet radiation resistance in partial diploids of Escherichia coli K-12. J Bacteriol. 1969 Nov;100(2):1118–1120. doi: 10.1128/jb.100.2.1118-1120.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walker J. R., Ussery C. L., Allen J. S. Bacterial cell division regulation: lysogenization of conditional cell division lon - mutants of Escherichia coli by bacteriophage. J Bacteriol. 1973 Mar;113(3):1326–1332. doi: 10.1128/jb.113.3.1326-1332.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zipser D., Bhavsar P. Missense mutations in the lacZ gene that result in degradation of beta-galactosidase structural protein. J Bacteriol. 1976 Sep;127(3):1538–1542. doi: 10.1128/jb.127.3.1538-1542.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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