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. 1978 Mar;133(3):1073–1077. doi: 10.1128/jb.133.3.1073-1077.1978

Alpha-l-arabinofuranosidase from Rhodotorula flava.

E Uesaka, M Sato, M Raiju, A Kaji
PMCID: PMC222135  PMID: 25260

Abstract

An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5 and at 30 degrees C. The apparent Km and Vmax values of the enzyme for phenyl alpha-L-arabinofuranoside were determined to be 9.1 mM and 72.5 mumol per min per mg of protein, respectively.

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Selected References

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  1. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  2. Kaji A., Ichimi T. Production and properties of galactosidases from Corticium rolfsii. Appl Microbiol. 1969 Dec;18(6):1036–1040. doi: 10.1128/am.18.6.1036-1040.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kaji A., Saheki T. Endo-arabinanase from Bacillus subtilis F-11. Biochim Biophys Acta. 1975 Dec 18;410(2):354–360. doi: 10.1016/0005-2744(75)90237-5. [DOI] [PubMed] [Google Scholar]
  4. Kaji A., Tagawa K. Purification, crystallization and amino acid composition of alpha-L-arabinofuranosidase from Aspergillus niger. Biochim Biophys Acta. 1970 Jun 23;207(3):456–464. doi: 10.1016/s0005-2795(70)80008-3. [DOI] [PubMed] [Google Scholar]
  5. Kaji A., Talawa K., Ichimi T. Properties of purified alpha-L-arabinofuranosidase from Aspergillus niger. Biochim Biophys Acta. 1969 Jan 7;171(1):186–188. doi: 10.1016/0005-2744(69)90118-1. [DOI] [PubMed] [Google Scholar]
  6. Kaji A., Yoshihara O. Production and Properties of alpha-L-Arabinofuranosidase from Corticium rolfsii. Appl Microbiol. 1969 Jun;17(6):910–913. doi: 10.1128/am.17.6.910-913.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. VERLY W. G., KINNEY J. M., DU VIGNEAUD V. Effect of folic acid and leucovorin on synthesis of the labile methyl group from methanol in the rat. J Biol Chem. 1952 May;196(1):19–23. [PubMed] [Google Scholar]

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