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. 1978 Mar;133(3):1437–1443. doi: 10.1128/jb.133.3.1437-1443.1978

Turnover-synthesis of chloroplast DNA in developing chloroplasts.

A M Walfield, C L Hershberger
PMCID: PMC222182  PMID: 417068

Abstract

The mechanism for the turnover-synthesis of chloroplast DNA in the absence of net synthesis during the chloroplast maturation in Euglena gracilis was determined. DNA synthesis was measured by incorporation of32Pi into chloroplast DNA. The density label, 15N, was incorporated to examine the mechanism of turnover-synthesis. The newly synthesized segments represent a replacement of segments in the DNA containing 1.5 X 10(3) to 6.1 X 10(3) nucleotides. Twenty-three fragments of chloroplast DNA, generated by digestion with the restriction endonuclease EcoRI, became labeled with 32Pi. Turnover-synthesis, therefore, replaces segments throughout the molecule of chloroplast DNA.

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Selected References

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