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. 1978 Apr;134(1):147–156. doi: 10.1128/jb.134.1.147-156.1978

Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.

J Lopilato, T Tsuchiya, T H Wilson
PMCID: PMC222229  PMID: 25882

Abstract

Thiomethyl-beta-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of Escherichia coli. TMG uptake by either intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5 and 8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The alpha-galactosides, melibiose, methyl-alpha-galactoside, and o-nitrophenyl-alpha-galactoside had a high affinity for this system whereas lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.

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Selected References

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  1. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burstein C., Kepes A. The alpha-galactosidase from Escherichia coli K12. Biochim Biophys Acta. 1971 Jan 26;230(1):52–63. doi: 10.1016/0304-4165(71)90053-5. [DOI] [PubMed] [Google Scholar]
  3. COHEN G. N., RICKENBERG H. V. Concentration spécifique réversible des amino acides chez Escherichia coli. Ann Inst Pasteur (Paris) 1956 Nov;91(5):693–720. [PubMed] [Google Scholar]
  4. Drapeau G. R., Matula T. I., MacLeod R. A. Nutrition and metabolism of marine bacteria. XV. Relation of Na+-activated transport to the Na+ requirement of a marine pseudomonad for growth. J Bacteriol. 1966 Jul;92(1):63–71. doi: 10.1128/jb.92.1.63-71.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Frank L., Hopkins I. Sodium-stimulated transport of glutamate in Escherichia coli. J Bacteriol. 1969 Oct;100(1):329–336. doi: 10.1128/jb.100.1.329-336.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  7. Halpern Y. S., Barash H., Dover S., Druck K. Sodium and potassium requirements for active transport of glutamate by Escherichia coli K-12. J Bacteriol. 1973 Apr;114(1):53–58. doi: 10.1128/jb.114.1.53-58.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hasan S. M., Tsuchiya T. Glutamate transport driven by an electrochemical gradient of sodium ion in membrane vesicles of Escherichia coli B. Biochem Biophys Res Commun. 1977 Sep 9;78(1):122–128. doi: 10.1016/0006-291x(77)91229-3. [DOI] [PubMed] [Google Scholar]
  9. Hirata H., Kosmakos F. C., Brodie A. F. Active transport of proline in membrane preparations from Mycobacterium phlei. J Biol Chem. 1974 Nov 10;249(21):6965–6970. [PubMed] [Google Scholar]
  10. Kahane S., Marcus M., Barash H., Halpern Y. S. Sodium-dependent glutamate transport in membrane vesicles of Escherichia coli K-12. FEBS Lett. 1975 Aug 15;56(2):235–239. doi: 10.1016/0014-5793(75)81099-4. [DOI] [PubMed] [Google Scholar]
  11. Kawasaki T., Kayama Y. Effect of lithium on proline transport by whole cells of Escherichia coli. Biochem Biophys Res Commun. 1973 Nov 1;55(1):52–59. doi: 10.1016/s0006-291x(73)80058-0. [DOI] [PubMed] [Google Scholar]
  12. Kayama Y., Kawasaki T. Stimulatory effect of lithium ion on proline transport by whole cells of Escherichia coli. J Bacteriol. 1976 Oct;128(1):157–164. doi: 10.1128/jb.128.1.157-164.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kitada M., Horikoshi K. Sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. J Bacteriol. 1977 Sep;131(3):784–788. doi: 10.1128/jb.131.3.784-788.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koyama N., Kiyomiya A., Nosoh Y. Na+-dependent uptake of amino acids by an alkalophilic Bacillus. FEBS Lett. 1976 Dec 15;72(1):77–78. doi: 10.1016/0014-5793(76)80816-2. [DOI] [PubMed] [Google Scholar]
  15. Lanyi J. K., Yearwood-Drayton V., MacDonald R. E. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. I. Kinetics of the light-dependent and the sodium-gradient-dependent uptake. Biochemistry. 1976 Apr 20;15(8):1595–1603. doi: 10.1021/bi00653a001. [DOI] [PubMed] [Google Scholar]
  16. MacDonald R. E., Greene R. V., Lanyi J. K. Light-activated amino acid transport systems in Halobacterium halobium envelope vesicles: role of chemical and electrical gradients. Biochemistry. 1977 Jul 12;16(14):3227–3235. doi: 10.1021/bi00633a029. [DOI] [PubMed] [Google Scholar]
  17. MacDonald R. E., Lanyi J. K., Greene R. V. Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3167–3170. doi: 10.1073/pnas.74.8.3167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MacDonald R. E., Lanyi L. K. Light-induced leucine transport in Halobacterium halobium envelope vesicles: a chemiosmotic system. Biochemistry. 1975 Jul;14(13):2882–2889. doi: 10.1021/bi00684a014. [DOI] [PubMed] [Google Scholar]
  19. MacLeod R. A., Thurman P., Rogers H. J. Comparative transport activity of intact cells, membrane vesicles, and mesosomes of Bacillus licheniformis. J Bacteriol. 1973 Jan;113(1):329–340. doi: 10.1128/jb.113.1.329-340.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Miner K. M., Frank L. Sodium-stimulated glutamate transport in osmotically shocked cells and membrane vesicles of Escherichia coli. J Bacteriol. 1974 Mar;117(3):1093–1098. doi: 10.1128/jb.117.3.1093-1098.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PARDEE A. B. An inducible mechanism for accumulation of melibiose in Escherichia coli. J Bacteriol. 1957 Mar;73(3):376–385. doi: 10.1128/jb.73.3.376-385.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PRESTIDGE L. S., PARDEE A. B. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 May 4;100:591–593. doi: 10.1016/0304-4165(65)90029-2. [DOI] [PubMed] [Google Scholar]
  24. Pearce S. M., Hildebrandt V. A., Lee T. Third system for neutral amino acid transport in a marine pseudomonad. J Bacteriol. 1977 Apr;130(1):37–47. doi: 10.1128/jb.130.1.37-47.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rosen B. P. Beta-galactoside transport and proton movements in an adenosine triphosphatase-deficient mutant of Escherichia coli. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1289–1296. doi: 10.1016/0006-291x(73)90605-0. [DOI] [PubMed] [Google Scholar]
  26. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  27. Schmitt R. Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12. J Bacteriol. 1968 Aug;96(2):462–471. doi: 10.1128/jb.96.2.462-471.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schmitt R., Rotman B. Alpha-galactosidase activity in cell-free extracts of Escherichia coli. Biochem Biophys Res Commun. 1966 Mar 8;22(5):473–479. doi: 10.1016/0006-291x(66)90297-x. [DOI] [PubMed] [Google Scholar]
  29. Shiio I., Miyajima R., Kashima N. Na+-dependent transport of threonine in Brevibacterium flavum. J Biochem. 1973 Jun;73(6):1185–1193. doi: 10.1093/oxfordjournals.jbchem.a130190. [DOI] [PubMed] [Google Scholar]
  30. Sprott G. D., Drozdowski J. P., Martin E. L., MacLeod R. A. Kinetics of Naplus-dependent amino acid transport using cells and membrane vesicles of a marine pseudomonad. Can J Microbiol. 1975 Jan;21(1):43–50. doi: 10.1139/m75-006. [DOI] [PubMed] [Google Scholar]
  31. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]
  32. Thompson J., MacLeod R. A. Functions of Na+ and K+ in the active transport of -aminoisobutyric acid in a marine pseudomonad. J Biol Chem. 1971 Jun 25;246(12):4066–4074. [PubMed] [Google Scholar]
  33. Thompson J., MacLeod R. A. Na+ and K+ gradients and alpha-aminoisobutyric acid transport in a marine pseudomonad. J Biol Chem. 1973 Oct 25;248(20):7106–7111. [PubMed] [Google Scholar]
  34. Tokuda H., Kaback H. R. Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium. Biochemistry. 1977 May 17;16(10):2130–2136. doi: 10.1021/bi00629a013. [DOI] [PubMed] [Google Scholar]
  35. Tsuchiya T., Hasan S. M., Raven J. Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli. J Bacteriol. 1977 Sep;131(3):848–853. doi: 10.1128/jb.131.3.848-853.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Tsuchiya T., Raven J., Wilson T. H. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli. Biochem Biophys Res Commun. 1977 May 9;76(1):26–31. doi: 10.1016/0006-291x(77)91663-1. [DOI] [PubMed] [Google Scholar]
  37. Willis R. C., Furlong C. E. Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli. J Biol Chem. 1975 Apr 10;250(7):2581–2586. [PubMed] [Google Scholar]
  38. Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]
  39. Wong P. T., Thompson J., MacLeod R. A. Nutrition and metabolism of marine bacteria. XVII. Ion-dependent retention of alpha-aminoisobutyric acid and its relation to Na+ dependent transport in a marine pseudomonad. J Biol Chem. 1969 Feb 10;244(3):1016–1025. [PubMed] [Google Scholar]

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