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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 May;134(2):418–422. doi: 10.1128/jb.134.2.418-422.1978

Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa.

F A Mian, T R Jarman, R C Righelato
PMCID: PMC222268  PMID: 96088

Abstract

In batch cultures of Pseudomonas aeruginosa, the maximum rate of exopolysaccharide synthesis occurred during exponential growth. In nitrogen-limited continuous culture, the specific rate of exopolysaccharide synthesis increased from 0.27 g g of cell-1 h-1 at a dilution rate (D) of 0.05 h-1 to 0.44 g g of cells h-1 at D=0.1 H-1. The yield of exopolysaccharide on the basis of glucose used was in the range of 56 to 64%. Exopolysaccharide was also synthesized in carbon-limited cultures at 0.19 g g of cell-1 h-1 at D=0.05 h-1 in a 33% yield. Nonmucoid variants appeared after seven generations in continuous culture and rapidly increased in proportion to the total number of organisms present.

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Selected References

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