Abstract
In batch cultures of Pseudomonas aeruginosa, the maximum rate of exopolysaccharide synthesis occurred during exponential growth. In nitrogen-limited continuous culture, the specific rate of exopolysaccharide synthesis increased from 0.27 g g of cell-1 h-1 at a dilution rate (D) of 0.05 h-1 to 0.44 g g of cells h-1 at D=0.1 H-1. The yield of exopolysaccharide on the basis of glucose used was in the range of 56 to 64%. Exopolysaccharide was also synthesized in carbon-limited cultures at 0.19 g g of cell-1 h-1 at D=0.05 h-1 in a 33% yield. Nonmucoid variants appeared after seven generations in continuous culture and rapidly increased in proportion to the total number of organisms present.
Full text
PDF![418](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e499/222268/db2e748499f8/jbacter00294-0072.png)
![419](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e499/222268/7aa87b847ef0/jbacter00294-0073.png)
![420](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e499/222268/fc25d3055231/jbacter00294-0074.png)
![421](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e499/222268/155c884b509e/jbacter00294-0075.png)
![422](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e499/222268/ad698ada59f5/jbacter00294-0076.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cadmus M. C., Rogovin S. P., Burton K. A., Pittsley J. E., Knutson C. A., Jeanes A. Colonial variation in Xanthomonas campestris NRRL B-1459 and characterization of the polysaccharide from a variant strain. Can J Microbiol. 1976 Jul;22(7):942–948. doi: 10.1139/m76-136. [DOI] [PubMed] [Google Scholar]
- Doggett R. G., Harrison G. M., Carter R. E. Mucoid Pseudomonas aeruginosa in patients with chronic illnesses. Lancet. 1971 Jan 30;1(7692):236–237. doi: 10.1016/s0140-6736(71)90973-1. [DOI] [PubMed] [Google Scholar]
- Evans L. R., Linker A. Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa. J Bacteriol. 1973 Nov;116(2):915–924. doi: 10.1128/jb.116.2.915-924.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goto S., Murakawa T., Kuwahara S. Slime production by Pseudomonas aeruginosa. II. A new synthetic medium and cultural conditions suitable for slime production by Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Jan;17(1):45–51. doi: 10.1111/j.1348-0421.1973.tb00702.x. [DOI] [PubMed] [Google Scholar]
- Govan J. R. Mucoid strains of Pseudomonas aeruginosa: the influence of culture medium on the stability of mucus production. J Med Microbiol. 1975 Nov;8(4):513–522. doi: 10.1099/00222615-8-4-513. [DOI] [PubMed] [Google Scholar]
- HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
- Larsen B., Haug A. Biosynthesis of alginate. 1. Composition and structure of alginate produced by Azotobacter vinelandii (Lipman). Carbohydr Res. 1971 Apr;17(2):287–296. doi: 10.1016/s0008-6215(00)82536-7. [DOI] [PubMed] [Google Scholar]
- Marks M. I., Prentice R., Swarson R., Cotton E. K., Eickhoff T. C. Carbenicillin and gentamicin: pharmacologic studies in patients with cystic fibrosis and pseudomonas pulmonary infections. J Pediatr. 1971 Nov;79(5):822–828. doi: 10.1016/s0022-3476(71)80401-8. [DOI] [PubMed] [Google Scholar]
- Martin D. R. Mucoid variation in Pseudomonas aeruginosa induced by the action of phage. J Med Microbiol. 1973 Feb;6(1):111–118. doi: 10.1099/00222615-6-1-111. [DOI] [PubMed] [Google Scholar]
- Morris E. R., Rees D. A., Young G., Walkinshaw M. D., Darke A. Order-disorder transition for a bacterial polysaccharide in solution. A role for polysaccharide conformation in recognition between Xanthomonas pathogen and its plant host. J Mol Biol. 1977 Feb 15;110(1):1–16. doi: 10.1016/s0022-2836(77)80095-8. [DOI] [PubMed] [Google Scholar]
- Neijssel O. M., Tempest D. W. The regulation of carbohydrate metabolism in Klebsiella aerogenes NCTC 418 organisms, growing in chemostat culture. Arch Microbiol. 1975 Dec 31;106(3):251–258. doi: 10.1007/BF00446531. [DOI] [PubMed] [Google Scholar]
- Sutherland I. W. Bacterial exopolysaccharides. Adv Microb Physiol. 1972;8:143–213. doi: 10.1016/s0065-2911(08)60190-3. [DOI] [PubMed] [Google Scholar]
- Zierdt C. H., Schmidt P. J. Dissociation in Pseudomonas aeruginosa. J Bacteriol. 1964 May;87(5):1003–1010. doi: 10.1128/jb.87.5.1003-1010.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]