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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 May;134(2):629–635. doi: 10.1128/jb.134.2.629-635.1978

Stable denaturation of chromosomal DNA from Saccharomyces cerevisiae during meiosis.

H L Klein, B Byers
PMCID: PMC222296  PMID: 350830

Abstract

Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time course of their appearance indicated that the denatured regions were present during the periods of premeiotic DNA replication and recombination. A similar pattern of denaturation was also detected in the DNA from vegetatively grown cells of a conditional cdc8 mutant, which is defective in DNA replication.

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Selected References

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