Abstract
This work reports on the isolation and characterization of two glutamine auxotrophs in Neurospora crassa. The mutations responsible for the glutamine-requiring phenotype were very closely linked, and one of them proved to be recessive to wild type. The mutations impaired the conversion of glutamic acid to glutamine and resulted in changes of both the activity and oligomeric structure of the enzyme glutamine synthetase.
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Selected References
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- Dubois E. L., Grenson M. Absence of involvement of glutamine synthetase and of NAD-linked glutamate dehydrogenase in the nitrogen catabolite repression of arginase and other enzymes in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1974 Sep 9;60(1):150–157. doi: 10.1016/0006-291x(74)90185-5. [DOI] [PubMed] [Google Scholar]
- Dubois E., Vissers S., Grenson M., Wiame J. M. Glutamine and ammonia in nitrogen catabolite repression of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1977 Mar 21;75(2):233–239. doi: 10.1016/0006-291x(77)91033-6. [DOI] [PubMed] [Google Scholar]
- Ferguson A. R., Sims A. P. The regulation of glutamine metabolism in Candida utilis: the inactivation of glutamine synthetase. J Gen Microbiol. 1974 Jan;80(1):173–185. doi: 10.1099/00221287-80-1-173. [DOI] [PubMed] [Google Scholar]
- Ferguson A. R., Sims A. P. The regulation of glutamine metabolism in Candida utilis: the role of glutamine in the control of glutamine synthetase. J Gen Microbiol. 1974 Jan;80(1):159–171. doi: 10.1099/00221287-80-1-159. [DOI] [PubMed] [Google Scholar]
- Foor F., Janssen K. A., Magasanik B. Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4844–4848. doi: 10.1073/pnas.72.12.4844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Limón-Lason J., Lara M., Resendiz B., Mora J. Regulation of glutamine synthetase in fed-batch cultures of Neurospora crassa. Biochem Biophys Res Commun. 1977 Oct 24;78(4):1234–1240. doi: 10.1016/0006-291x(77)91425-5. [DOI] [PubMed] [Google Scholar]
- Magasanik B., Prival M. J., Brenchley J. E., Tyler B. M., DeLeo A. B., Streicher S. L., Bender R. A., Paris C. G. Glutamine synthetase as a regulator of enzyme synthesis. Curr Top Cell Regul. 1974;8(0):119–138. doi: 10.1016/b978-0-12-152808-9.50010-9. [DOI] [PubMed] [Google Scholar]
- Palacios R. Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure. J Biol Chem. 1976 Aug 10;251(15):4787–4791. [PubMed] [Google Scholar]
- Prival M. J., Brenchley J. E., Magasanik B. Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes. J Biol Chem. 1973 Jun 25;248(12):4334–4344. [PubMed] [Google Scholar]
- Quinto C., Mora J., Palacios R. Neurospora crassa glutamine synthetase. Role of enzyme synthesis and degradation on the regulation of enzyme concentration during exponential growth. J Biol Chem. 1977 Dec 10;252(23):8724–8727. [PubMed] [Google Scholar]
- Streicher S. L., Bender R. A., Magasanik B. Genetic control of glutamine synthetase in Klebiella aerogenes. J Bacteriol. 1975 Jan;121(1):320–331. doi: 10.1128/jb.121.1.320-331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaca G., Mora J. Nitrogen regulation of arginase in Neurospora crassa. J Bacteriol. 1977 Sep;131(3):719–725. doi: 10.1128/jb.131.3.719-725.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weisbrod R. E., Meister A. Studies on glutamine synthetase from Escherichia coli. Formation of pyrrolidone carboxylate and inhibition by methionine sulfoximine. J Biol Chem. 1973 Jun 10;248(11):3997–4002. [PubMed] [Google Scholar]