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. 1978 Jun;134(3):737–743. doi: 10.1128/jb.134.3.737-743.1978

Sodium ion-proton antiport in a marine bacterium.

D F Niven, R A MacLeod
PMCID: PMC222318  PMID: 26666

Abstract

Alteromonas haloplanktis ejected protons in response to a brief respiratory pulse; the rate of decay of the resulting pH change was accelerated when Na+ was present in the suspension medium. The addition of an anaerobic NaCl solution to an essentially Na+-free anaerobic bacterial suspension induced the acidification of the suspension medium. These results and others discussed provide substantial evidence for the existence of an Na+-H+ antiporter in this organism.

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Selected References

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  1. Ariel M., Grossowicz N. Enhancement of transport in Micrococcus lysodeikticus by sodium ions. Biochim Biophys Acta. 1974 May 30;352(1):122–126. doi: 10.1016/0005-2736(74)90184-9. [DOI] [PubMed] [Google Scholar]
  2. Buckmire F. L., MacLeod R. A. Nutrition and metabolism of marine bacteria. XIV. On the mechanism of lysis of a marine bacterium. Can J Microbiol. 1965 Aug;11(4):677–691. doi: 10.1139/m65-091. [DOI] [PubMed] [Google Scholar]
  3. Drapeau G. R., Matula T. I., MacLeod R. A. Nutrition and metabolism of marine bacteria. XV. Relation of Na+-activated transport to the Na+ requirement of a marine pseudomonad for growth. J Bacteriol. 1966 Jul;92(1):63–71. doi: 10.1128/jb.92.1.63-71.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenbach M., Cooper S., Garty H., Johnstone R. M., Rottenberg H., Caplan S. R. Light-driven sodium transport in sub-bacterial particles of Halobacterium halobium. Biochim Biophys Acta. 1977 Mar 17;465(3):599–613. doi: 10.1016/0005-2736(77)90276-0. [DOI] [PubMed] [Google Scholar]
  5. Frank L., Hopkins I. Sodium-stimulated transport of glutamate in Escherichia coli. J Bacteriol. 1969 Oct;100(1):329–336. doi: 10.1128/jb.100.1.329-336.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Halpern Y. S., Barash H., Dover S., Druck K. Sodium and potassium requirements for active transport of glutamate by Escherichia coli K-12. J Bacteriol. 1973 Apr;114(1):53–58. doi: 10.1128/jb.114.1.53-58.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hasan S. M., Tsuchiya T. Glutamate transport driven by an electrochemical gradient of sodium ion in membrane vesicles of Escherichia coli B. Biochem Biophys Res Commun. 1977 Sep 9;78(1):122–128. doi: 10.1016/0006-291x(77)91229-3. [DOI] [PubMed] [Google Scholar]
  8. Hassan H. M., MacLeod R. A. Kinetics of Na+-dependent K+ ion transport in a marine pseudomonad. J Bacteriol. 1975 Jan;121(1):160–164. doi: 10.1128/jb.121.1.160-164.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kahane S., Marcus M., Barash H., Halpern Y. S. Sodium-dependent glutamate transport in membrane vesicles of Escherichia coli K-12. FEBS Lett. 1975 Aug 15;56(2):235–239. doi: 10.1016/0014-5793(75)81099-4. [DOI] [PubMed] [Google Scholar]
  10. Lanyi J. K., MacDonald R. E. Existence of electrogenic hydrogen ion/sodium ion antiport in Halobacterium halobium cell envelope vesicles. Biochemistry. 1976 Oct 19;15(21):4608–4614. doi: 10.1021/bi00666a010. [DOI] [PubMed] [Google Scholar]
  11. Lanyi J. K., Renthal R., MacDonald R. E. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. II. Evidence that the driving force is a light-dependent sodium gradient. Biochemistry. 1976 Apr 20;15(8):1603–1610. doi: 10.1021/bi00653a002. [DOI] [PubMed] [Google Scholar]
  12. Lanyi J. K., Yearwood-Drayton V., MacDonald R. E. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. I. Kinetics of the light-dependent and the sodium-gradient-dependent uptake. Biochemistry. 1976 Apr 20;15(8):1595–1603. doi: 10.1021/bi00653a001. [DOI] [PubMed] [Google Scholar]
  13. MacDonald R. E., Greene R. V., Lanyi J. K. Light-activated amino acid transport systems in Halobacterium halobium envelope vesicles: role of chemical and electrical gradients. Biochemistry. 1977 Jul 12;16(14):3227–3235. doi: 10.1021/bi00633a029. [DOI] [PubMed] [Google Scholar]
  14. MacDonald R. E., Lanyi J. K., Greene R. V. Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3167–3170. doi: 10.1073/pnas.74.8.3167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MacDonald R. E., Lanyi L. K. Light-induced leucine transport in Halobacterium halobium envelope vesicles: a chemiosmotic system. Biochemistry. 1975 Jul;14(13):2882–2889. doi: 10.1021/bi00684a014. [DOI] [PubMed] [Google Scholar]
  16. Miner K. M., Frank L. Sodium-stimulated glutamate transport in osmotically shocked cells and membrane vesicles of Escherichia coli. J Bacteriol. 1974 Mar;117(3):1093–1098. doi: 10.1128/jb.117.3.1093-1098.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  18. Mitchell P. Performance and conservation of osmotic work by proton-coupled solute porter systems. J Bioenerg. 1973 Jan;4(1):63–91. doi: 10.1007/BF01516051. [DOI] [PubMed] [Google Scholar]
  19. Sprott G. D., Drozdowski J. P., Martin E. L., MacLeod R. A. Kinetics of Naplus-dependent amino acid transport using cells and membrane vesicles of a marine pseudomonad. Can J Microbiol. 1975 Jan;21(1):43–50. doi: 10.1139/m75-006. [DOI] [PubMed] [Google Scholar]
  20. Sprott G. D., MacLeod R. A. Na + -dependent amino acid transport in isolated membrane vesicles of a marine pseudomonad energized by electron donors. Biochem Biophys Res Commun. 1972 May 26;47(4):838–845. doi: 10.1016/0006-291x(72)90569-4. [DOI] [PubMed] [Google Scholar]
  21. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]
  22. TAKACS F. P., MATULA T. I., MACLEOD R. A. NUTRITION AND METABOLISM OF MARINE BACTERIA. XIII. INTRACELLULAR CONCENTRATIONS OF SODIUM AND POTASSIUM IONS IN A MARINE PSEUDOMONAD. J Bacteriol. 1964 Mar;87:510–518. doi: 10.1128/jb.87.3.510-518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thompson J., MacLeod R. A. Na+ and K+ gradients and alpha-aminoisobutyric acid transport in a marine pseudomonad. J Biol Chem. 1973 Oct 25;248(20):7106–7111. [PubMed] [Google Scholar]
  24. Tokuda H., Kaback H. R. Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium. Biochemistry. 1977 May 17;16(10):2130–2136. doi: 10.1021/bi00629a013. [DOI] [PubMed] [Google Scholar]
  25. Tsuchiya T., Hasan S. M., Raven J. Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli. J Bacteriol. 1977 Sep;131(3):848–853. doi: 10.1128/jb.131.3.848-853.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tsuchiya T., Raven J., Wilson T. H. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli. Biochem Biophys Res Commun. 1977 May 9;76(1):26–31. doi: 10.1016/0006-291x(77)91663-1. [DOI] [PubMed] [Google Scholar]
  27. West I. C., Mitchell P. Proton/sodium ion antiport in Escherichia coli. Biochem J. 1974 Oct;144(1):87–90. doi: 10.1042/bj1440087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wong P. T., Thompson J., MacLeod R. A. Nutrition and metabolism of marine bacteria. XVII. Ion-dependent retention of alpha-aminoisobutyric acid and its relation to Na+ dependent transport in a marine pseudomonad. J Biol Chem. 1969 Feb 10;244(3):1016–1025. [PubMed] [Google Scholar]

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