Abstract
Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal map as determined by conjugational and transductional analyses. Two phenotypic classes of Les- mutants were identified. One class of mutations has pleiotropic effects on DNA metabolism. These mutants are unable to recombine genetic material acquired as a result of either conjugation or transduction (Rec-). In addition, the ability of these Les- Rec- mutants to repair UV-induced damage to bacteriophage is reduced (host-cell reactivation deficient, Hcr-). Mutants of the second class are Les-, Rec+, and Hcr+.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Booker R. J., Loutit J. S. The order of replication of chromosomal markers in Pseudomonas aeruginosa strain 1. I. Marker frequency analysis by transduction. Genet Res. 1974 Apr;23(2):145–153. doi: 10.1017/s0016672300014762. [DOI] [PubMed] [Google Scholar]
- Brooks K., Clark A. J. Behavior of lambda bacteriophage in a recombination deficienct strain of Escherichia coli. J Virol. 1967 Apr;1(2):283–293. doi: 10.1128/jvi.1.2.283-293.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chandler P. M., Krishnapillai V. Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa. Mutat Res. 1974 Apr;23(1):15–23. doi: 10.1016/0027-5107(74)90155-9. [DOI] [PubMed] [Google Scholar]
- HOLLOWAY B. W., MONK M., HODGINS L., FARGIE B. Effects of radiation on transduction on Pseudomonas aeruginosa. Virology. 1962 Sep;18:89–94. doi: 10.1016/0042-6822(62)90180-0. [DOI] [PubMed] [Google Scholar]
- Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
- Herskowitz I. Control of gene expression in bacteriophage lambda. Annu Rev Genet. 1973;7:289–324. doi: 10.1146/annurev.ge.07.120173.001445. [DOI] [PubMed] [Google Scholar]
- Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holloway B. W. Mutants of Pseudomonas aeruginosa with reduced recombination ability. Mutat Res. 1966 Oct;3(5):452–455. doi: 10.1016/0027-5107(66)90055-8. [DOI] [PubMed] [Google Scholar]
- Holloway B. W. Radiation-sensitive mutants of Pseudomonas aeruginosa with reduced host-cell reactivation of bacteriophages. Mutat Res. 1966 Apr;3(2):167–171. doi: 10.1016/0027-5107(66)90031-5. [DOI] [PubMed] [Google Scholar]
- Hong J. S., Smith G. R., Ames B. N. Adenosine 3':5'-cyclic monophosphate concentration in the bacterial host regulates the viral decision between lysogeny and lysis. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2258–2262. doi: 10.1073/pnas.68.9.2258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horii Z., Clark A. J. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J Mol Biol. 1973 Oct 25;80(2):327–344. doi: 10.1016/0022-2836(73)90176-9. [DOI] [PubMed] [Google Scholar]
- Loutit J. S., Marinus M. G. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet Res. 1968 Aug;12(1):37–44. doi: 10.1017/s0016672300011599. [DOI] [PubMed] [Google Scholar]
- Loutit J. S., Marinus M. G., Pearce L. E. Investigation of the mating system of Pseudomonas aeruginosa strain 1. 3. Kinetic studies on the transfer of the sex factor (FP). Genet Res. 1968 Oct;12(2):139–145. doi: 10.1017/s0016672300011757. [DOI] [PubMed] [Google Scholar]
- Miller R. V., Pemberton J. M., Clark A. J. Prophage F116: evidence for extrachromosomal location in Pseudomonas aeruginosa strain PAO. J Virol. 1977 Jun;22(3):844–847. doi: 10.1128/jvi.22.3.844-847.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moody E. E., Hayes W. Chromosome transfer by autonomous transmissible plasmids: the role of the bacterial recombination (rec) system. J Bacteriol. 1972 Jul;111(1):80–85. doi: 10.1128/jb.111.1.80-85.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pemberton J. M., Clark A. J. Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973 Apr;114(1):424–433. doi: 10.1128/jb.114.1.424-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pemberton J. M., Holloway B. W. Chromosome mapping in Pseudomonas aeruginosa. Genet Res. 1972 Jun;19(3):251–260. doi: 10.1017/s0016672300014518. [DOI] [PubMed] [Google Scholar]
- Signer E. R. Lysogeny: the integration problem. Annu Rev Microbiol. 1968;22:451–488. doi: 10.1146/annurev.mi.22.100168.002315. [DOI] [PubMed] [Google Scholar]
- Threlkeld S. F., Stoltz J. M. A genetic analysis of non-disjunction and mitotic recombination in Neurospora crassa. Genet Res. 1970 Aug;16(1):29–35. doi: 10.1017/s0016672300002251. [DOI] [PubMed] [Google Scholar]
- van de Putte P., Holloway B. W. A thermosensitive recombination deficient mutant of Pseudomonas aeruginosa. Mutat Res. 1968 Sep-Oct;6(2):195–203. doi: 10.1016/0027-5107(68)90034-1. [DOI] [PubMed] [Google Scholar]