Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Jun;134(3):902–912. doi: 10.1128/jb.134.3.902-912.1978

Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2.

P L Kuempel, S A Duerr, P D Maglothin
PMCID: PMC222337  PMID: 350855

Abstract

Escherichia coli CRT4624-P2sig5 is a dnaA mutant in which integration of the prophage P2sig5 has occurred at the attP2II site (min 85). This strain was integratively suppressed, and when cells were shifted to 42 degrees C replication was initiated at a site in or near the P2 prophage. Initially, this replication occurred primarily in the direction that corresponds to the clockwise direction on the genetic map. Replication also occurred in the counterclockwise direction, but the initiation of replication in this direction occurred approximately 40 min later than the initiation of replication in the other direction. Because of this delay, the replication forks that traveled in the clockwise direction were the first to arrive in the region of the replication terminus. These replication forks ceased replication near the aroD locus (min 37), and it is proposed that the replication terminus is between the aroD and rac loci (min 31). A model is proposed for the cycle of chromosome replication in this strain at 42 degrees C.

Full text

PDF
902

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beyersmann D., Messer W., Schlicht M. Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. J Bacteriol. 1974 Jun;118(3):783–789. doi: 10.1128/jb.118.3.783-789.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bird R. E., Chandler M., Caro L. Suppression of an Escherichia coli dnaA mutation by the integrated R factor R.100.1: Change of chromosome replication origin in synchronized cultures. J Bacteriol. 1976 Jun;126(3):1215–1223. doi: 10.1128/jb.126.3.1215-1223.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bird R. E., Louarn J., Martuscelli J., Caro L. Origin and sequence of chromosome replication in Escherichia coli. J Mol Biol. 1972 Oct 14;70(3):549–566. doi: 10.1016/0022-2836(72)90559-1. [DOI] [PubMed] [Google Scholar]
  5. Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
  6. Chandler M., Silver L., Caro L. Suppression of an Escherichia coli dnaA mutation by the integrated R factor R100.1: origin of chromosome replication during exponential growth. J Bacteriol. 1977 Aug;131(2):421–430. doi: 10.1128/jb.131.2.421-430.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  8. Edlund T., Gustafsson P., Wolf-Watz H. Effect of thymine concentration on the mode of chromosomal replication in Escherichia coli K-12. J Mol Biol. 1976 Dec;108(2):295–303. doi: 10.1016/s0022-2836(76)80122-2. [DOI] [PubMed] [Google Scholar]
  9. Hohlfeld R., Vielmetter W. Bidirectional growth of the E. coli chromosome. Nat New Biol. 1973 Apr 4;242(118):130–132. doi: 10.1038/newbio242130a0. [DOI] [PubMed] [Google Scholar]
  10. Kuempel P. L. Deoxyribonucleic acid-deoxyribonucleic acid hybridization assay for replication origin deoxyribonucleic acid of Escherichia coli. J Bacteriol. 1972 Jun;110(3):917–925. doi: 10.1128/jb.110.3.917-925.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuempel P. L., Duerr S. A., Seeley N. R. Terminus region of the chromosome in Escherichia coli inhibits replication forks. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3927–3931. doi: 10.1073/pnas.74.9.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lane H. E., Denhardt D. T. The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome. J Bacteriol. 1974 Nov;120(2):805–814. doi: 10.1128/jb.120.2.805-814.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lindahl G. Bacteriophage P2: replication of the chromosome requires a protein which acts only on the genome that coded for it. Virology. 1970 Oct;42(2):522–533. doi: 10.1016/0042-6822(70)90295-3. [DOI] [PubMed] [Google Scholar]
  14. Lindahl G., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407–2411. doi: 10.1073/pnas.68.10.2407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Louarn J., Patte J., Louarn J. M. Evidence for a fixed termination site of chromosome replication in Escherichia coli K12. J Mol Biol. 1977 Sep 25;115(3):295–314. doi: 10.1016/0022-2836(77)90156-5. [DOI] [PubMed] [Google Scholar]
  16. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nishimura Y., Caro L., Berg C. M., Hirota Y. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol. 1971 Feb 14;55(3):441–456. doi: 10.1016/0022-2836(71)90328-7. [DOI] [PubMed] [Google Scholar]
  18. Schnös M., Inman R. B. Starting point and direction of replication in P2 DNA. J Mol Biol. 1971 Jan 14;55(1):31–38. doi: 10.1016/0022-2836(71)90278-6. [DOI] [PubMed] [Google Scholar]
  19. Silver L., Chandler M., de la Tour E. B., Caro L. Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures. J Bacteriol. 1977 Sep;131(3):929–942. doi: 10.1128/jb.131.3.929-942.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sueoka N., Yoshikawa H. The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics. 1965 Oct;52(4):747–757. doi: 10.1093/genetics/52.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES