Abstract
Yeast extract was treated with tyrosine decarboxylase and used to prepare a rich, complex medium virtually free of tyrosine. The medium supported maximal growth rates for Escherichia coli prototrophs, as well as for defined and undefined auxotrophs. It has made possible the efficient radiochemical labeling of cells growing optimally in complex medium and the characterization of mutants with undefined requirements. Similarly prepared media may be useful for the study of fastidious organisms and organisms for which no defined medium has been described.
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- BUHLER D. R. A simple scintillation counting technique for assaying C1402 in a Warburg flask. Anal Biochem. 1962 Nov;4:413–417. doi: 10.1016/0003-2697(62)90143-4. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bellamy W. D., Gunsalus I. C. Tyrosine Decarboxylation by Streptococci: Growth Requirements for Active Cell Production. J Bacteriol. 1944 Aug;48(2):191–199. doi: 10.1128/jb.48.2.191-199.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
- Clarke S. J., Low B., Konigsberg W. H. Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12. J Bacteriol. 1973 Mar;113(3):1091–1095. doi: 10.1128/jb.113.3.1091-1095.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duberstein R., Oeschger M. P. Growth of bacteriophage H on male and female strains of Escherichia coli. J Virol. 1973 Mar;11(3):460–463. doi: 10.1128/jvi.11.3.460-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
- Hirota Y., Jacob F., Ryter A., Buttin G., Nakai T. On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria. J Mol Biol. 1968 Jul 14;35(1):175–192. doi: 10.1016/s0022-2836(68)80046-4. [DOI] [PubMed] [Google Scholar]
- Oeschger M. P., Berlyn M. K. Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees. Proc Natl Acad Sci U S A. 1975 Mar;72(3):911–915. doi: 10.1073/pnas.72.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oeschger M. P., Woods S. L. A temperature-sensitive suppressor enabling the manipulation of the level of individual proteins in intact cells. Cell. 1976 Feb;7(2):205–212. doi: 10.1016/0092-8674(76)90019-2. [DOI] [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]