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. 1978 Jun;134(3):1176–1178. doi: 10.1128/jb.134.3.1176-1178.1978

Does cycloheximide-induced loss of phosphate uptake activity in Neurospora crassa reflect rapid turnover?

R E Beever, D J Burns
PMCID: PMC222368  PMID: 149111

Abstract

Addition of cycloheximide to Neurospora crassa germlings growing in liquid medium caused an exponential loss of phosphate uptake activity (half-life, ca. 2 h). No loss of activity resulted when germlings were resuspended, at the time of cycloheximide addition, in medium of a substantially lower phosphate concentration. It is concluded that the phosphate uptake systems are not subject to rapid turnover.

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Selected References

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  1. Beever R. E., Burns D. J. Adaptive changes in phosphate uptake by the fungus Neurospora crassa in response to phosphate supply. J Bacteriol. 1977 Nov;132(2):520–525. doi: 10.1128/jb.132.2.520-525.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burns D. J., Beever R. E. Kinetic characterization of the two phosphate uptake systems in the fungus Neurospora crassa. J Bacteriol. 1977 Nov;132(2):511–519. doi: 10.1128/jb.132.2.511-519.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elsas L. J., Rosenberg L. E. Inhibition of amino Acid transport in rat kidney cortex by puromycin. Proc Natl Acad Sci U S A. 1967 Feb;57(2):371–378. doi: 10.1073/pnas.57.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grenson M., Crabeel M., Wiame J. M., Béchet J. Inhibition of protein synthesis and simulation of permease turnover in yeast. Biochem Biophys Res Commun. 1968 Feb 26;30(4):414–419. doi: 10.1016/0006-291x(68)90760-2. [DOI] [PubMed] [Google Scholar]
  5. Gross W., Ring K. Effect of chloramphenicol on active amino acid transport. FEBS Lett. 1969 Aug;4(4):319–322. doi: 10.1016/0014-5793(69)80265-6. [DOI] [PubMed] [Google Scholar]
  6. Horowitz N. H., Feldman H. M., Pall M. L. Derepression of tyrosinase synthesis in Neurospora by cycloheximide, actinomycin D, and puromycin. J Biol Chem. 1970 Jun 10;245(11):2784–2788. [PubMed] [Google Scholar]
  7. Hunter D. R., Segel I. H. Acidic and basic amino acid transport systems of Penicillium chrysogenum. Arch Biochem Biophys. 1971 May;144(1):168–183. doi: 10.1016/0003-9861(71)90466-8. [DOI] [PubMed] [Google Scholar]
  8. Lowendorf H. S., Bazinet G. F., Jr, Slayman C. W. Phosphate transport in Neurospora. Derepression of a high-affinity transport system during phosphorus starvation. Biochim Biophys Acta. 1975 May 21;389(3):541–549. doi: 10.1016/0005-2736(75)90164-9. [DOI] [PubMed] [Google Scholar]
  9. Lowendorf H. S., Slayman C. L., Slayman C. W. Phosphate transport in Neurospora. Kinetic characterization of a constitutive, low-affinity transport system. Biochim Biophys Acta. 1974 Dec 24;373(3):369–382. doi: 10.1016/0005-2736(74)90017-0. [DOI] [PubMed] [Google Scholar]
  10. Marzluf G. A. Control of the synthesis, activity, and turnover of enzymes of sulfur metabolism in Neurospora crassa. Arch Biochem Biophys. 1972 Jun;150(2):714–724. doi: 10.1016/0003-9861(72)90090-2. [DOI] [PubMed] [Google Scholar]
  11. Pall M. L., Kelly K. A. Specificity of transinhibition of amino acid transport in neurospora. Biochem Biophys Res Commun. 1971 Mar 5;42(5):940–947. doi: 10.1016/0006-291x(71)90521-3. [DOI] [PubMed] [Google Scholar]
  12. Ring K., Gross W., Heinz E. Negative feedback regulation of amino acid transport in Streptomyces hydrogenans. Arch Biochem Biophys. 1970 Mar;137(1):243–252. doi: 10.1016/0003-9861(70)90431-5. [DOI] [PubMed] [Google Scholar]
  13. Wiley W. R., Matchett W. H. Tryptophan transport in Neurospora crassa. II. Metabolic control. J Bacteriol. 1968 Mar;95(3):959–966. doi: 10.1128/jb.95.3.959-966.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Williams S. K., 2nd, Hodson R. C. Transport of urea at low concentrations in Chlamydomonas reinhardi. J Bacteriol. 1977 Apr;130(1):266–273. doi: 10.1128/jb.130.1.266-273.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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