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. 1978 Jun;134(3):1188–1191. doi: 10.1128/jb.134.3.1188-1191.1978

Approximation of the cell cycle in synchronized populations of Streptococcus faecium.

R P Hinks, L Daneo-Moore, G D Shockman
PMCID: PMC222372  PMID: 96099

Abstract

Slowly growing populations (TD = 70 to 80 min) of Streptococcus faecium (S. faecalis ATCC 9790) were synchronized by selection after sucrose gradient fractionation. The cell cycle was approximated by correlating the patterns of DNA accumulation and cell division. More specifically, the beginning of cell cycle was equated with the beginning of a rapid linear increase in DNA accumulation. The DNA content of the culture approximately doubled during the period of accumulation, which lasted about 51 min. The period of rapid DNA accumulation, was followed by a period of reduced accumulation that lasted about 24 min. During synchronized growth, cell numbers increased rapidly in coordination with the period of rapid DNA accumulation and exhibited a plateau during the period of reduced DNA accumulation. In contrast, RNA and protein appeared to accumulate exponentially throughout the cell cycle at the same rate as culture mass.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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