Abstract
A mutant strain of Azotobacter vinelandii that is unable to fix N2 (Nif-) was transformed to Nif+ with DNA from Rhizobium japonicum. Of 50 Nif+ transformants tested, 3 contained the O antigen-related polysaccharide that is present on the cell surface of a nodulating R. japonicum strain, but is absent from a non-nodulating mutant strain.
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- Bishop P. E., Dazzo F. B., Appelbaum E. R., Maier R. J., Brill W. J. Intergeneric transfer of genes involved in the Rhizobium-legume symbiosis. Science. 1977 Dec 2;198(4320):938–940. doi: 10.1126/science.929179. [DOI] [PubMed] [Google Scholar]
- Bohlool B. B., Schmidt E. L. Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis. Science. 1974 Jul 19;185(4147):269–271. doi: 10.1126/science.185.4147.269. [DOI] [PubMed] [Google Scholar]
- Dazzo F. B., Brill W. J. Receptor site on clover and alfalfa roots for Rhizobium. Appl Environ Microbiol. 1977 Jan;33(1):132–136. doi: 10.1128/aem.33.1.132-136.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dazzo F. B., Hubbell D. H. Cross-reactive antigens and lectin as determinants of symbiotic specificity in the Rhizobium-clover association. Appl Microbiol. 1975 Dec;30(6):1017–1033. doi: 10.1128/am.30.6.1017-1033.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maier R. J., Brill W. J. Ineffective and non-nodulating mutant strains of Rhizobium japonicum. J Bacteriol. 1976 Aug;127(2):763–769. doi: 10.1128/jb.127.2.763-769.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maier R. J., Brill W. J. Involvement of Rhizobium japonicum O antigen in soybean nodulation. J Bacteriol. 1978 Mar;133(3):1295–1299. doi: 10.1128/jb.133.3.1295-1299.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Page W. J., Sadoff H. L. Physiological factors affecting transformation of Azotobacter vinelandii. J Bacteriol. 1976 Mar;125(3):1080–1087. doi: 10.1128/jb.125.3.1080-1087.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shah V. K., Davis I. C., Gordon J. K., Orme-Johnson W. H., Brill W. J. Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra. Biochim Biophys Acta. 1973 Jan 18;292(1):246–255. doi: 10.1016/0005-2728(73)90269-7. [DOI] [PubMed] [Google Scholar]
- Strandberg G. W., Wilson P. W. Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. Can J Microbiol. 1968 Jan;14(1):25–31. doi: 10.1139/m68-005. [DOI] [PubMed] [Google Scholar]
- Wolpert J. S., Albersheim P. Host-symbiont interactions. I. The lectins of legumes interact with the o-antigen-containing lipopolysaccharides of their symbiont Rhizobia. Biochem Biophys Res Commun. 1976 Jun 7;70(3):729–737. doi: 10.1016/0006-291x(76)90653-7. [DOI] [PubMed] [Google Scholar]