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. 1978 Aug;135(2):295–299. doi: 10.1128/jb.135.2.295-299.1978

Specificity and Regulation of γ-Aminobutyrate Transport in Escherichia coli

S Kahane 1, R Levitz 1, Y S Halpern 1
PMCID: PMC222382  PMID: 28310

Abstract

A specific γ-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a Km of 12 μM and a Vmax of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the ability to utilize GABA as a nitrogen source, although the activities of glutamate-succinylsemialdehyde transaminase (GSST) (EC 2.6.1.19) and succinylsemialdehyde dehydrogenase (SSDH) (EC 1.2.1.16), the enzymes that catalyze GABA utilization, remained as high as in the parental CS101B strain. The ability to utilize l-ornithine, l-arginine, putrescine, l-proline, and glycine as a nitrogen source was preserved in the mutants. The genetic lesions resulting in the loss of GABA transport, gabP5 and gabP9, mapped in the gab gene cluster in close linkage to gabT and gabD, the structural genes of GSST and SSDH, and to gabC, a gene controlling the utilization of GABA, arginine, putrescine, and ornithine. The synthesis of the GABA transport carrier is subject to dual physiological control by (i) catabolite repression and (ii) nitrogen availability. Experiments with glutamine synthetase (EC 6.3.1.2)-negative and with glutamine synthetase-constitutive strains strongly indicate that this enzyme is the effector in the regulation of GABA carrier synthesis by route (ii).

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Selected References

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  1. BURROUS S. E., DEMOSS R. D. STUDIES ON TRYPTOPHAN PERMEASE IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 6;73:623–637. doi: 10.1016/0006-3002(63)90332-9. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Betteridge P. R., Ayling P. D. The regulation of glutamine transport and glutamine synthetase in Salmonella typhimurium. J Gen Microbiol. 1976 Aug;96(2):324–334. doi: 10.1099/00221287-95-2-324. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dover S., Halpern Y. S. Control of the pathway of -aminobutyrate breakdown in Escherichia coli K-12. J Bacteriol. 1972 Apr;110(1):165–170. doi: 10.1128/jb.110.1.165-170.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dover S., Halpern Y. S. Utilization of -aminobutyric acid as the sole carbon and nitrogen source by Escherichia coli K-12 mutants. J Bacteriol. 1972 Feb;109(2):835–843. doi: 10.1128/jb.109.2.835-843.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halpern Y. S., Lupo M. Glutamate transport in wild-type and mutant strains of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1288–1295. doi: 10.1128/jb.90.5.1288-1295.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee M., Robbins J. C., Oxender D. L. Transport properties of merodiploids covering the dagA locus in Escherichia coli K-12. J Bacteriol. 1975 Jun;122(3):1001–1005. doi: 10.1128/jb.122.3.1001-1005.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lo T. C., Rayman M. K., Sanwal B. D. Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. J Biol Chem. 1972 Oct 10;247(19):6323–6331. [PubMed] [Google Scholar]
  10. Quay S. C., Oxender D. L. Regulation of branched-chain amino acid transport in Escherichia coli. J Bacteriol. 1976 Sep;127(3):1225–1238. doi: 10.1128/jb.127.3.1225-1238.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosen B. P. Basic amino acid transport in Escherichia coli: properties of canavanine-resistant mutants. J Bacteriol. 1973 Nov;116(2):627–635. doi: 10.1128/jb.116.2.627-635.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tyler B., Deleo A. B., Magasanik B. Activation of transcription of hut DNA by glutamine synthetase. Proc Natl Acad Sci U S A. 1974 Jan;71(1):225–229. doi: 10.1073/pnas.71.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Willis R. C., Iwata K. K., Furlong C. E. Regulation of Glutamine Transport in Escherichia coli. J Bacteriol. 1975 Jun;122(3):1032–1037. doi: 10.1128/jb.122.3.1032-1037.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zaboura M., Halpern Y. S. Regulation of gamma-aminobutyric acid degradation in Escherichia coli by nitrogen metabolism enzymes. J Bacteriol. 1978 Feb;133(2):447–451. doi: 10.1128/jb.133.2.447-451.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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