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. 1978 Aug;135(2):300–306. doi: 10.1128/jb.135.2.300-306.1978

Further characterization of the R plasmid Rts1 and its mutant pTW2: replication and incompatibility of the plasmid.

Y Terawaki, Y Kobayashi
PMCID: PMC222383  PMID: 355218

Abstract

Incompatibility of the R plasmid Rts1 and its replication mutant pTW2 was studied in recA host cells of Escherichia coli. When the R plasmid R401, belonging to the same incompatibility group as Rts1, was used as a test plasmid, R401 was eliminated preferentially from (Rts-R401)+ cells irrespective of the direction of transfer. In contrast, pTW2 and R401 were mutually excluded. The decreased incompatibility of pTW2 was confirmed by a direct incompatibility test in which a derivative of Rts1 expelled pTW2 exclusively. Alkaline sucrose gradients of pTW2 and Rts1 DNA indicated that approximately one-fourth of the Rts1 genome was deleted in pTW2. In addition, both the various temperature-dependent properties of Rts1 and the inhibitory effect on phage T4 development were also lost in pTW2. A possible mechanism that regulates the stringent replication of Rts1 is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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