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. 1955 Nov 25;1(6):583–604. doi: 10.1083/jcb.1.6.583

THE FINE STRUCTURE OF CORTICAL COMPONENTS OF PARAMECIUM MULTIMICRONUCLEATUM

Albert W Sedar 1, Keith R Porter 1
PMCID: PMC2223830  PMID: 13278368

Abstract

The electron microscope was used to study the structure and three dimensional relationships of the components of the body cortex in thin sections of Paramecium multimicronucleatum. Micrographs of sections show that the cortex is covered externally by two closely apposed membranes (together ∼250 A thick) constituting the pellicle. Beneath the pellicle the surface of the animal is molded into ridges that form a polygonal ridgework with depressed centers. It is these ridges that give the surface of the organism its characteristic configuration and correspond to the outer fibrillar system of the light microscope image. The outer ends of the trichocysts with their hood-shaped caps are located in the centers of the anterior and posterior ridges of each polygon. The cilia extend singly from the depressed centers of the surface polygons. Each cilium shows two axial filaments with 9 peripheral and parallel filaments embedded in a matrix and the whole surrouned by a thin ciliary membrane. The 9 peripheral filaments are double and these are evenly spaced in a circle around the central pair. The ciliary membrane is continuous with the outer member of the pellicular membrane, whereas the plasma membrane is continuous with the inner member of the pellicular membrane. At the level of the plasma membrane the proximal end of the cilium is continuous with its tube-shaped basal body or kinetosome. The peripheral filaments of the cilium, together with the material of cortical matrix which tends to condense around them, form the sheath of the basal body. The kinetodesma connecting the ciliary kinetosomes (inner fibrillar system of the light microscopist) is composed of a number of discrete fibrils which overlap in a shingle-like fashion. Each striated kinetosomal fibril originates from a ciliary kinetosome and runs parallel to other kinetosomal fibrils arising from posterior kinetosomes of a particular meridional array. Sections at the level of the ciliary kinetosomes reveal an additional fiber system, the infraciliary lattice system, which is separate and distinct from the kinetodesmal system. This system consists of a fibrous network of irregular polygons and runs roughly parallel to the surface of the animal. Mitochondria have a fine structure similar in general features to that described for a number of mammalian cell types, but different in certain details. The structures corresponding to cristae mitochondriales appear as finger-like projections or microvilli extending into the matrix of the organelle from the inner membrane of the paired mitochondrial membrane. The cortical cytoplasm contains also a particulate component and a system of vesicles respectively comparable to the nucleoprotein particles and to the endoplasmic reticulum described in various metazoan cell types. An accessory kinetosome has been observed in oblique sections of a number of non-dividing specimens slightly removed from the ciliary kinetosome and on the same meridional line as the cilia and trichocysts. Its position corresponds to the location of the kinetosome of the newly formed cilium in animals selected as being in the approaching fission stage of the life cycle.

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Selected References

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  1. BEAMS H. W., TAHMISIAN T. N., DEVINE R. L. Electron microscope studies on the cells of the malpighian tubules of the grasshopper (Orthoptera, Acrididae). J Biophys Biochem Cytol. 1955 May 25;1(3):197–202. doi: 10.1083/jcb.1.3.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BEAMS H. W., TAHMISIAN T. N. Structure of the mitochondria in the male germ cells of Helix as revealed by the electron microscopy. Exp Cell Res. 1954 Feb;6(1):87–93. doi: 10.1016/0014-4827(54)90151-2. [DOI] [PubMed] [Google Scholar]
  3. BRETSCHNEIDER L. H. Elektronenmikro-skopische Untersuchung einiger Ziliaten. Mikroskopie. 1950;5(11-12):257–269. [PubMed] [Google Scholar]
  4. BURBRING E., BURN J. H., SHELLEY H. J. Acetylcholine and ciliary movement in the gill plates of Mytilus edulis. Proc R Soc Lond B Biol Sci. 1953 Sep;141(905):445–466. doi: 10.1098/rspb.1953.0053. [DOI] [PubMed] [Google Scholar]
  5. CHALLICE C. E. Electron microscope studies of spermiogenesis in some rodents. J R Microsc Soc. 1953 Sep;73(3):115–127. doi: 10.1111/j.1365-2818.1953.tb01978.x. [DOI] [PubMed] [Google Scholar]
  6. FAWCETT D. W. Observations on the cytology and electron microscopy of hepatic cells. J Natl Cancer Inst. 1955 Apr;15(5 Suppl):1475–1503. [PubMed] [Google Scholar]
  7. KORDIK P., BULBRING E., BURN J. H. Ciliary movement and acetylcholine. Br J Pharmacol Chemother. 1952 Mar;7(1):67–79. doi: 10.1111/j.1476-5381.1952.tb00691.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KRUGER F., WOHLFARTH-BOTTERMANN K. E. Elektronenoptische Beobachtungen an Ciliatenorganellen. Mikroskopie. 1952;7(3-4):121–130. [PubMed] [Google Scholar]
  9. PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  11. PALADE G. E., PORTER K. R. Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954 Dec 1;100(6):641–656. doi: 10.1084/jem.100.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PALADE G. E. The fine structure of mitochondria. Anat Rec. 1952 Nov;114(3):427–451. doi: 10.1002/ar.1091140304. [DOI] [PubMed] [Google Scholar]
  13. PORTER K. R., BLUM J. A study in microtomy for electron microscopy. Anat Rec. 1953 Dec;117(4):685–710. doi: 10.1002/ar.1091170403. [DOI] [PubMed] [Google Scholar]
  14. PORTER K. R. Electron microscopy of basophilic components of cytoplasm. J Histochem Cytochem. 1954 Sep;2(5):346–375. doi: 10.1177/2.5.346. [DOI] [PubMed] [Google Scholar]
  15. PORTER K. R. Observations on a submicroscopic basophilic component of cytoplasm. J Exp Med. 1953 May;97(5):727–750. doi: 10.1084/jem.97.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SEAMAN G. R., HOULIHAN R. K. Enzyme systems in Tetrahymena geleii S. II. Acetylcholinesterase activity; its relation to motility of the organism and to coordinated ciliary action in general. J Cell Physiol. 1951 Apr;37(2):309–321. doi: 10.1002/jcp.1030370210. [DOI] [PubMed] [Google Scholar]
  17. SEAMAN G. R. Localization of acetylcholinesterase activity in the protozoan, Tetrahymena geleii S. Proc Soc Exp Biol Med. 1951 Jan;76(1):169–170. doi: 10.3181/00379727-76-18425. [DOI] [PubMed] [Google Scholar]
  18. SEDAR A. W., BEAMS H. W., JANNEY C. D. Electron microscope studies on the ciliary apparatus of the gill cells of Mya arenaria. Proc Soc Exp Biol Med. 1952 Feb;79(2):303–305. doi: 10.3181/00379727-79-19359. [DOI] [PubMed] [Google Scholar]
  19. WEINREB S., HARMAN J. W. Phase and electron microscope studies of the interrelationship of cytochondria and myofibrils in pigeon breast muscle. J Exp Med. 1955 May 1;101(5):529–537. doi: 10.1084/jem.101.5.529. [DOI] [PMC free article] [PubMed] [Google Scholar]

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