Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Aug;135(2):348–353. doi: 10.1128/jb.135.2.348-353.1978

Enterobacterial common antigen in mutant strains of Salmonella.

D Männel, P H Mäkelä, H Mayer
PMCID: PMC222390  PMID: 355222

Abstract

A good correlation was found to exist between the serologically determined presence of enterobacterial common antigen (ECA) and the amount of the rare sugar constituent D-mannosaminuronic acid. Strains classified by serological techniques as ECA+, ECA-, and ECAtrace were found to possess the expected amounts of mannosaminuronic acid in the ECA-enriched phenol-soluble PL-L fractions. This correlation provides strong evidence on the identity of the mannosaminuronic acid-glucosamine polymer with the ECA as defined by Kunin (J. Exp. Med. 118:565-586, 1963).

Full text

PDF
348

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
  2. Fromme I., Beilharz H. Gas chromatographic assay of total and O-acetyl groups in bacterial lipopolysaccharides. Anal Biochem. 1978 Feb;84(2):347–353. doi: 10.1016/0003-2697(78)90051-9. [DOI] [PubMed] [Google Scholar]
  3. Galanos C., Lüderitz O. Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975 Jun;54(2):603–610. doi: 10.1111/j.1432-1033.1975.tb04172.x. [DOI] [PubMed] [Google Scholar]
  4. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  5. Johns M. A., Whiteside R. E., Baker E. E., McCabe W. R. Common enterobacterial antigen. I. Isolation and purification from Salmonella typhose 0:901. J Immunol. 1973 Mar;110(3):781–790. [PubMed] [Google Scholar]
  6. Jousimies H., Mäkelä P. H. Genetic analysis of Salmonella minnesota R mutants with defects in the biosynthesis of the lipopolysaccharide core. J Bacteriol. 1974 Sep;119(3):753–759. doi: 10.1128/jb.119.3.753-759.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KUNIN C. M. SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE. J Exp Med. 1963 Oct 1;118:565–586. doi: 10.1084/jem.118.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lüderitz O., Galanos C., Risse H. J., Ruschmann E., Schlecht S., Schmidt G., Schulte-Holthausen H., Wheat R., Westphal O., Schlosshardt J. Structural relationship of Salmonella O and R antigens. Ann N Y Acad Sci. 1966 Jun 30;133(2):349–374. doi: 10.1111/j.1749-6632.1966.tb52376.x. [DOI] [PubMed] [Google Scholar]
  9. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  10. Marx A., Petcovici M., Nacescu N., Mayer H., Schmidt G. Demonstration of enterobacterial common antigen by bacterial agglutination. Infect Immun. 1977 Dec;18(3):563–567. doi: 10.1128/iai.18.3.563-567.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mayer H. D-Mannosaminuronsäure-Baustein des K7-antigens von Escherichia coli. Eur J Biochem. 1969 Mar;8(1):139–145. doi: 10.1111/j.1432-1033.1969.tb00506.x. [DOI] [PubMed] [Google Scholar]
  12. Mayer H., Schmidt G. Hämagglutinine gegen ein gemeinsames Enterobacteriaceen-Antigen in E. coli R1-Antiseren. Zentralbl Bakteriol Orig. 1971;216(3):299–313. [PubMed] [Google Scholar]
  13. Mayer H., Westphal O. Elektrophoretische Trennungen von Hexosamin- und Hexuronsäurederivaten als Molybdatkomplexe. J Chromatogr. 1968 Mar 19;33(3):514–525. doi: 10.1016/s0021-9673(00)98681-3. [DOI] [PubMed] [Google Scholar]
  14. Mäkelä P. H., Mayer H. Enterobacterial common antigen. Bacteriol Rev. 1976 Sep;40(3):591–632. doi: 10.1128/br.40.3.591-632.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mäkelä P. H., Mayer H., Whang H. Y., Neter E. Participation of lipopolysaccharide genes in the determination of the enterobacterial common antigen: analysis of R mutants of Salmonella minnesota. J Bacteriol. 1974 Sep;119(3):760–764. doi: 10.1128/jb.119.3.760-764.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mäkelä P. H. Participation of lipopolysaccharide genes in the determination of the entobacterial common antigen: analysis in Salmonella groups B and C1. J Bacteriol. 1974 Sep;119(3):765–770. doi: 10.1128/jb.119.3.765-770.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mäkelä P. H., Schmidt G., Mayer H., Nikaido H., Whang H. Y., Neter E. Enterobacterial common antigen in rfb deletion mutants of Salmonella typhimurium. J Bacteriol. 1976 Sep;127(3):1141–1149. doi: 10.1128/jb.127.3.1141-1149.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Männel D., Mayer H. Isolation and chemical characterization of the enterobacterial common antigen. Eur J Biochem. 1978 May 16;86(2):361–370. doi: 10.1111/j.1432-1033.1978.tb12318.x. [DOI] [PubMed] [Google Scholar]
  19. Männel D., Mayer H. Serological and immunological properties of isolated enterobacterial common antigen. Eur J Biochem. 1978 May 16;86(2):371–379. doi: 10.1111/j.1432-1033.1978.tb12319.x. [DOI] [PubMed] [Google Scholar]
  20. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  22. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  23. Schlecht S. Wachstum und Lipopolysaccharidgehalt von Salmonellen bei Submerszüchtungen nach dem Batch-Verfahren. 2. Mitteilung: Einfluss der Wachstumsphase auf die Lipopolysaccharidsynthese. Zentralbl Bakteriol Orig A. 1975 Jun;232(1):61–72. [PubMed] [Google Scholar]
  24. Schmidt G., Mayer H., Mäkelä P. H. Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen. J Bacteriol. 1976 Aug;127(2):755–762. doi: 10.1128/jb.127.2.755-762.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Whang H. Y., Loza U., Neter E., Milgrom F. Gel precipitation of common enterobacterial antigen by its antibody. Int Arch Allergy Appl Immunol. 1973;45(6):905–914. doi: 10.1159/000231089. [DOI] [PubMed] [Google Scholar]
  26. Whang H. Y., Mayer H., Schmidt G., Neter E. Immunogenicity of the common enterobacterial antigen produced by smooth and rough strains. Infect Immun. 1972 Oct;6(4):533–539. doi: 10.1128/iai.6.4.533-539.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES