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. 1997;76(2):234–237. doi: 10.1038/bjc.1997.367

Stereologically estimated mean nuclear volume of prostatic cancer is a reliable prognostic parameter.

K Arima 1, Y Sugimura 1, T Hioki 1, A Yamashita 1, J Kawamura 1
PMCID: PMC2223932  PMID: 9231924

Abstract

Although different histological grading systems of prostatic cancer refer to well-described characteristics, results are hard to reproduce. The aim of this study was to obtain morphometric data that would enable objective and reproducible grading of prostatic cancers by stereological estimation of mean nuclear volume (MNV). The clinical records and tissue specimens from 100 patients who were newly diagnosed as having prostatic cancer from 1973 to 1990 and who were followed up for 5 years or longer were retrospectively examined. We analysed the relationship between MNV and clinical stage, Gleason score and histological grading according to the World Health Organization (WHO) classification. To evaluate prognostic predictors, a multivariate analysis of factors associated with cause-specific survival was performed. We found a good correlation between the MNV and clinical stage and between the MNV and histological grading. There was no correlation between MNVs and Gleason scores. Multivariate analysis revealed that the MNV was the only predictor of survival time (coefficient 0.005; P < 0.0001; hazard ratio 1.005). We consider that the MNV is an excellent predictor of the prognosis in patients with prostatic cancer. Moreover, stereological estimation of MNV is a simple, quick, inexpensive and reliable morphometric procedure that enables the quantitative analysis of the histological and biological character of prostatic cancer.

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Selected References

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  1. Arima K., Sugimura Y., Tochigi H., Kawamura J. Mean nuclear volume of bladder cancer: stereological estimation and its clinical value. Urol Int. 1993;51(2):62–66. doi: 10.1159/000282515. [DOI] [PubMed] [Google Scholar]
  2. Braendgaard H., Gundersen H. J. The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods. 1986 Oct;18(1-2):39–78. doi: 10.1016/0165-0270(86)90112-3. [DOI] [PubMed] [Google Scholar]
  3. Böcking A., Kiehn J., Heinzel-Wach M. Combined histologic grading of prostatic carcinoma. Cancer. 1982 Jul 15;50(2):288–294. doi: 10.1002/1097-0142(19820715)50:2<288::aid-cncr2820500220>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  4. Cintra M. L., Billis A. Histologic grading of prostatic adenocarcinoma: intraobserver reproducibility of the Mostofi, Gleason and Böcking grading systems. Int Urol Nephrol. 1991;23(5):449–454. doi: 10.1007/BF02583988. [DOI] [PubMed] [Google Scholar]
  5. Epstein J. I., Carmichael M. J., Pizov G., Walsh P. C. Influence of capsular penetration on progression following radical prostatectomy: a study of 196 cases with long-term followup. J Urol. 1993 Jul;150(1):135–141. doi: 10.1016/s0022-5347(17)35415-0. [DOI] [PubMed] [Google Scholar]
  6. Fujikawa K., Sasaki M., Aoyama T., Itoh T. Prognostic criteria in patients with stage D2 prostate cancer. Correlation with mean nuclear volume. Cancer. 1995 Jul 1;76(1):91–95. doi: 10.1002/1097-0142(19950701)76:1<91::aid-cncr2820760113>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  7. Gundersen H. J., Jensen E. B. Stereological estimation of the volume-weighted mean volume of arbitrary particles observed on random sections. J Microsc. 1985 May;138(Pt 2):127–142. doi: 10.1111/j.1365-2818.1985.tb02607.x. [DOI] [PubMed] [Google Scholar]
  8. Jörgensen P. E., Berild G. H., Bruun E., Weis N., Nielsen K., Gundersen H. J. Stereological estimates of nuclear volume correlated with clinical stage and progression of prostatic cancer: clinical aspects. Scand J Urol Nephrol Suppl. 1988;110:131–135. [PubMed] [Google Scholar]
  9. Nielsen K., Berild G. H., Bruun E., Jorgensen P. E., Weis N. Stereological estimation of mean nuclear volume in prostatic cancer, the reproducibility and the possible value of estimations on repeated biopsies in the course of disease. J Microsc. 1989 Apr;154(Pt 1):63–69. doi: 10.1111/j.1365-2818.1989.tb00568.x. [DOI] [PubMed] [Google Scholar]
  10. Nielsen K., Colstrup H., Nilsson T., Gundersen H. J. Stereological estimates of nuclear volume correlated with histopathological grading and prognosis of bladder tumour. Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;52(1):41–54. doi: 10.1007/BF02889949. [DOI] [PubMed] [Google Scholar]
  11. Nielsen K., Petersen S. E., Orntoft T. A comparison between stereological estimates of mean nuclear volume and DNA flow cytometry in bladder tumours. APMIS. 1989 Oct;97(10):949–956. doi: 10.1111/j.1699-0463.1989.tb00503.x. [DOI] [PubMed] [Google Scholar]
  12. Partin A. W., Steinberg G. D., Pitcock R. V., Wu L., Piantadosi S., Coffey D. S., Epstein J. I. Use of nuclear morphometry, gleason histologic scoring, clinical stage, and age to predict disease-free survival among patients with prostate cancer. Cancer. 1992 Jul 1;70(1):161–168. doi: 10.1002/1097-0142(19920701)70:1<161::aid-cncr2820700126>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  13. Song J., Cheng W. S., Cupps R. E., Earle J. D., Farrow G. M., Lieber M. M. Nuclear deoxyribonucleic acid content measured by static cytometry: important prognostic association for patients with clinically localized prostate carcinoma treated by external beam radiotherapy. J Urol. 1992 Mar;147(3 Pt 2):794–797. doi: 10.1016/s0022-5347(17)37388-3. [DOI] [PubMed] [Google Scholar]
  14. Svanholm H., Mygind H. Prostatic carcinoma reproducibility of histologic grading. Acta Pathol Microbiol Immunol Scand A. 1985 Mar;93(2):67–71. doi: 10.1111/j.1699-0463.1985.tb03921.x. [DOI] [PubMed] [Google Scholar]

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