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. 1956 Mar 25;2(2):159–170. doi: 10.1083/jcb.2.2.159

ELECTRON MICROSCOPY OF BASOPHILIC STRUCTURES OF SOME INVERTEBRATE OOCYTES

II. FINE STRUCTURE OF THE YOLK NUCLEI

Lionel I Rebhun 1
PMCID: PMC2223963  PMID: 13319379

Abstract

RNA containing yolk nuclei from the surf clam Spisula solidissima have been studied with the light microscope and with the electron microscope. A variety of structures can be seen with both and a correlation can be made between bodies studied with the electron microscope and those studied with the light microscope. The electron microscope shows many of these structures to be composed of double walled lamellae arranged in space, in various ways. The variety of patterns seen with the electron microscope can be satisfactorily explained on the basis of a hypothetical model. The presence of yolk platelets within the yolk nuclei lends support to classical observations on the synthesis of yolk within yolk nuclei or yolk nuclei derivatives. Small granules (about 100 A) are included within the double walled lamellae and their presence probably accounts for the basophilic nature of the entire body. The presence of small granules attached to electron-dense layers relates the yolk nuclei described here to ergastoplasm discussed by others.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHARD W., GAUTIER A., ROUILLER C. La notion de microsomes et le problème de la basophilie cytoplasmique; étude critique et expérimentale. Arch Anat Microsc Morphol Exp. 1954;43(3):236–275. [PubMed] [Google Scholar]
  2. CHAUDHRY H. S. Nucleolar activity in the oocytes of some marine teleostean fishes. J R Microsc Soc. 1951 Jul;71(1):87–93. doi: 10.1111/j.1365-2818.1951.tb01952.x. [DOI] [PubMed] [Google Scholar]
  3. CHAUDHRY H. S. [The yolk-nucleus of Balbiani in teleostean fishes]. Z Zellforsch Mikrosk Anat. 1953;37(5):455–466. doi: 10.1007/BF00319632. [DOI] [PubMed] [Google Scholar]
  4. FAHMY O. G. Oogenesis in the desert snail Eremina desertorum with special reference to vitellogenesis. Q J Microsc Sci. 1949 Jun;90(Pt 2)(2):159–181. [PubMed] [Google Scholar]
  5. FAUTREZ J., FAUTREZ-FIRLEFYN N. A propos des relations entre nucléole et cytoplasme dans l'oocyte de Cyclops strenuus. C R Seances Soc Biol Fil. 1953 Feb;147(3-4):351–352. [PubMed] [Google Scholar]
  6. GALE E. F., FOLKES J. P. Effect of nucleic acids on protein synthesis and amino-acid incorporation in disrupted staphylococcal cells. Nature. 1954 Jun 26;173(4417):1223–1227. doi: 10.1038/1731223a0. [DOI] [PubMed] [Google Scholar]
  7. HOWATSON A. F., HAM A. W. Electron microscope study of sections of two rat liver tumors. Cancer Res. 1955 Jan;15(1):62–69. [PubMed] [Google Scholar]
  8. MULNARD J. Etude morphologique et cytochimique de l'oogenèse chez Acanthoscelides obtectus Say (Bruchide-Coléoptère). Arch Biol (Liege) 1954;65(2):261–314. [PubMed] [Google Scholar]
  9. PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  11. PALADE G. E. The fine structure of mitochondria. Anat Rec. 1952 Nov;114(3):427–451. doi: 10.1002/ar.1091140304. [DOI] [PubMed] [Google Scholar]
  12. PANIJEL J. L'organisation du vitellus dans les oeufs d'amphibiens. Biochim Biophys Acta. 1950 Jun;5(3/4):343–357. doi: 10.1016/0006-3002(50)90181-8. [DOI] [PubMed] [Google Scholar]
  13. PORTER K. R. Electron microscopy of basophilic components of cytoplasm. J Histochem Cytochem. 1954 Sep;2(5):346–375. doi: 10.1177/2.5.346. [DOI] [PubMed] [Google Scholar]
  14. VAKAET L. Recherches cytologiques et cytochimiques sur l'organisation de l'oocyte I de Lebistes reticulatus. Arch Biol (Liege) 1955;66(1):1–73. [PubMed] [Google Scholar]
  15. WEISS J. M. The ergastoplasm; its fine structure and relation to protein synthesis as studied with the electron microscope in the pancreas of the Swiss albino mouse. J Exp Med. 1953 Dec;98(6):607–618. doi: 10.1084/jem.98.6.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WITTEK M. La vitellogénèse chez les amphibiens. Arch Biol (Liege) 1952;58(2):133–198. [PubMed] [Google Scholar]

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