Skip to main content
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1956 May 25;2(3):253–262. doi: 10.1083/jcb.2.3.253

AN ELECTRON MICROSCOPE STUDY OF THE EPITHELIUM IN THE NORMAL MATURE AND IMMATURE MOUSE CORNEA

Huntington Sheldon 1
PMCID: PMC2223976  PMID: 13331958

Abstract

1. An electron microscope study at high resolution of the corneal epithelium of the normal mature and immature mouse revealed new information regarding the submicroscopic appearance of these cells. 2. Two thin dense lines separated by a less dense area constituted the structure of the limiting surface membrane of epithelial cells; the thickness of this membrane was about 80 A. 3. Some differences in the appearance of the cytoplasm and mitochondria of cells from the immature mouse cornea and the appearance of the cytoplasm and mitochondria of cells from the adult mouse cornea were observed. 4. The basement membrane appeared as a dense band about 600 A wide separating the basal epithelial cells from the substantia propria. Suggestions of periodicity were seen in some phosphotungstic acid-treated specimens. 5. Round bodies believed to be bacteria were seen on the surface of the outer epithelial cells in the adult mouse cornea but not in the immature, unopened eye.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARGMANN W., KNOOP A., SCHIEBLER T. H. Histologische, cytochemische und elektronen-mikroskopische Untersuchungen am Nephron, mit Berücksichtigung der Mitochondrien. Z Zellforsch Mikrosk Anat. 1955;42(4-5):386–422. [PubMed] [Google Scholar]
  2. BIRCH-ANDERSEN A., MAALØE O., SJOSTRAND F. S. High-resolution electron micrographs of sections of E. coli. Biochim Biophys Acta. 1953 Nov;12(3):395–400. doi: 10.1016/0006-3002(53)90157-7. [DOI] [PubMed] [Google Scholar]
  3. BORYSKO E., BANG F. B. The fine structure of the chorio-allantoic membrane of the normal chick embryo. Bull Johns Hopkins Hosp. 1953 Apr;92(4):257–289. [PubMed] [Google Scholar]
  4. BRADFIELD J. R. Electron microscopic observations on bacterial nuclei. Nature. 1954 Jan 30;173(4396):184–186. doi: 10.1038/173184a0. [DOI] [PubMed] [Google Scholar]
  5. DALTON A. J., FELIX M. D. Cytologic and cytochemical characteristics of the Golgi substance of epithelial cells of the epididymis in situ, in homogenates and after isolation. Am J Anat. 1954 Mar;94(2):171–207. doi: 10.1002/aja.1000940202. [DOI] [PubMed] [Google Scholar]
  6. FRIEDENWALD J. S. Recent studies on corneal metabolism and growth: a review. Cancer Res. 1950 Aug;10(8):461–466. [PubMed] [Google Scholar]
  7. MURPHY J. S., BANG F. B. Observations with the electron microscope on cells of the chick chorio-allantoic membrane infected with influenza virus. J Exp Med. 1952 Mar;95(3):259–268. doi: 10.1084/jem.95.3.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  10. RHODIN J. Electron microscopy of the glomerular capillary wall. Exp Cell Res. 1955 Jun;8(3):572–574. doi: 10.1016/0014-4827(55)90136-1. [DOI] [PubMed] [Google Scholar]
  11. SELBY C. C. An electron microscope study of the epidermis of mammalian skin in thin sections. I. Dermo-epidermal junction and basal cell layer. J Biophys Biochem Cytol. 1955 Sep 25;1(5):429–444. doi: 10.1083/jcb.1.5.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SJOSTRAND F. S. Electron microscopy of mitochondria and cytoplasmic double membranes. Nature. 1953 Jan 3;171(4340):30–32. doi: 10.1038/171030a0. [DOI] [PubMed] [Google Scholar]
  13. SJOSTRAND F. S., HANZON V. Membrane structures of cytoplasm and mitochondria in exocrine cells of mouse pancreas as revealed by high resolution electron microscopy. Exp Cell Res. 1954 Nov;7(2):393–414. doi: 10.1016/s0014-4827(54)80086-3. [DOI] [PubMed] [Google Scholar]
  14. SJOSTRAND F. S., HANZON V. Ultrastructure of Golgi apparatus of exocrine cells of mouse pancreas. Exp Cell Res. 1954 Nov;7(2):415–429. doi: 10.1016/s0014-4827(54)80087-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES