Skip to main content
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1956 Sep 25;2(5):573–588. doi: 10.1083/jcb.2.5.573

THE DISTRIBUTION OF NEWLY SYNTHESIZED DNA IN MITOTIC DIVISION

Walter Plaut 1, Daniel Mazia 1
PMCID: PMC2223985  PMID: 13376635

Abstract

The chromosomes of Crepis capillaris were labelled with thymidine-2-C14 in their DNA fraction. Quantitative analysis of the distribution of newly synthesized DNA in postmetaphase stages of the division following the period of label incorporation led to the conclusion that the new DNA is not necessarily equally distributed by the mitotic process and that, therefore, chromosome duplication does not involve the equal partition of parental DNA. The implications of these findings with respect to DNA duplication are discussed. An attempt is made to translate the pattern of new DNA distribution into a probable number of units of synthesis per chromosome.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTLER J. A. The action of ionizing radiations on biological materials; facts and theories. Radiat Res. 1956 Jan;4(1):20–32. [PubMed] [Google Scholar]
  2. CONGER A. D., FAIRCHILD L. M. A quick-freeze method for making smear slides permanent. Stain Technol. 1953 Nov;28(6):281–283. doi: 10.3109/10520295309105555. [DOI] [PubMed] [Google Scholar]
  3. DAOUST R., BERTALANFFY F. D., LEBLOND C. P. The rates of desoxyribonucleic acid synthesis and mitosis in lung. J Biol Chem. 1954 Mar;207(1):405–410. [PubMed] [Google Scholar]
  4. FATTERPAKER P., MARFATIA U., SREENIVASAN A. Protective action of vitamin B12 in the hyperthyroid rat. Nature. 1955 Jul 23;176(4473):165–165. doi: 10.1038/176165a0. [DOI] [PubMed] [Google Scholar]
  5. MAZIA D., PLAUT W. S., ELLIS G. W. A method for the quantitative assessment of autoradiographs. Exp Cell Res. 1955 Oct;9(2):305–312. doi: 10.1016/0014-4827(55)90103-8. [DOI] [PubMed] [Google Scholar]
  6. McQUADE H. A., FRIEDKIN M., ATCHISON A. A. Chromosome aberrations caused by radioactive thymidine. Nature. 1955 Jun 11;175(4467):1038–1039. doi: 10.1038/1751038b0. [DOI] [PubMed] [Google Scholar]
  7. STENT G. S. Decay of incorporated radioactive phosphorus during reproduction of bacteriophage T2. J Gen Physiol. 1955 Jul 20;38(6):853–865. doi: 10.1085/jgp.38.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. THOMAS R. Réactions chimiques au cours de l'hydrolyse préalable à la réaction de Feulgen. Bull Soc Chim Biol (Paris) 1950;32(7-8):469–472. [PubMed] [Google Scholar]
  9. WATSON J. D., CRICK F. H. Genetical implications of the structure of deoxyribonucleic acid. Nature. 1953 May 30;171(4361):964–967. doi: 10.1038/171964b0. [DOI] [PubMed] [Google Scholar]
  10. WATSON J. D., CRICK F. H. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953 Apr 25;171(4356):737–738. doi: 10.1038/171737a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES