Abstract
A method for chemically fractionating lymphatic organs has been described. The method has been shown to be applicable to bovine palatine tonsils, sheep palatine tonsils, and bovine thymus. Approximately 50 per cent of the dry weight of tonsils and about 30 per cent of thymus has been found to be soluble in the 0.15 M NaCl extract. Four components have been isolated which together account for 65 per cent by weight of the material in the extracts. Four other components have been identified and partially defined by means of electrophoretic mobility, solubility, or some other chemical or physical property.
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Selected References
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- ABRAMS A., COHEN P. P. Electrophoretic and chemical characterization of human lymphoid tissue and calf thymus. J Biol Chem. 1949 Jan;177(1):439–449. [PubMed] [Google Scholar]
- ANDERSON A. J., MACLAGAN N. F. The isolation and estimation of urinary mucoproteins. Biochem J. 1955 Apr;59(4):638–644. doi: 10.1042/bj0590638. [DOI] [PMC free article] [PubMed] [Google Scholar]
- AYALA W., MOORE L. V., HESS E. L. The purple color reaction given by diphenylamine reagent. I. With normal and rheumatic fever sera. J Clin Invest. 1951 Jul;30(7):781–785. doi: 10.1172/JCI102492. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BAKAY B., KOLB J. J., TOENNIES G. On the component proteins of calf thymus nucleoprotein. Arch Biochem Biophys. 1955 Sep;58(1):144–168. doi: 10.1016/0003-9861(55)90101-6. [DOI] [PubMed] [Google Scholar]
- HARRIS T. N., MOORE D. H., FARBER M. Physicochemical studies on lymph and lymphocyte extracts from normal and stimulated lymphatic tissue. J Biol Chem. 1949 May;179(1):369–376. [PubMed] [Google Scholar]
- HESS E. L., CAMPBELL M., HERRANEN A. A protease present in extracts of a lymphatic organ. J Biol Chem. 1955 Jul;215(1):163–170. [PubMed] [Google Scholar]
- HESS E. L., YASNOFF D. S., LAGG S. The sensitivity to ultraviolet radiation of a simple protein isolated from a lymphatic organ. Arch Biochem Biophys. 1955 Aug;57(2):323–333. doi: 10.1016/0003-9861(55)90295-2. [DOI] [PubMed] [Google Scholar]
- PALADE G. E., SIEKEVITZ P. Liver microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Mar 25;2(2):171–200. doi: 10.1083/jcb.2.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PETERMANN M. L., HAMILTON M. G., MIZEN N. A. Electrophoretic analysis of the macromolecular nucleoprotein particles of mammalian cytoplasm. Cancer Res. 1954 Jun;14(5):360–366. [PubMed] [Google Scholar]
- ROBERTS S., WHITE A. Biochemical characterization of lymphoid tissue proteins. J Biol Chem. 1949 Mar;178(1):151–162. [PubMed] [Google Scholar]
