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. 1978 Aug;135(2):436–444. doi: 10.1128/jb.135.2.436-444.1978

Replication of bromodeoxyuridylate-substituted mitochondrial DNA in yeast.

J Leff, T R Eccleshall
PMCID: PMC222401  PMID: 355226

Abstract

The DNA of several strains of Saccharomyces cerevisiae was labeled by growing the culture in medium supplemented with thymidylate and bromodeoxyuridylate. It was thus possible to follow the course of mitochondrial DNA replication in density shift experiments by determining the buoyant density distribution of unreplicated and replicated DNAs in analytical CsCl gradients. DNA replication was followed for three generations after transfer of cultures from light medium to heavy medium and heavy medium to light medium. Under both conditions, the density shifts observed for mitochondrial DNA were those expected for semiconservative, nondispersive replication. This was further confirmed by analysis of the buoyant density of alkali-denatured hybrid mitochondrial DNA. With this method, no significant recombination between replicated and unreplicated DNA was detected after three generations of growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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