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. 1957 May 25;3(3):363–380. doi: 10.1083/jcb.3.3.363

EXPERIMENTALLY INDUCED CHROMOSOME ABERRATIONS IN PLANTS

I. THE PRODUCTION OF CHROMOSOME ABERRATIONS BY CYANIDE AND OTHER HEAVY METAL COMPLEXING AGENTS

B A Kihlman 1
PMCID: PMC2224028  PMID: 13438921

Abstract

The finding of Lilly and Thoday that potassium cyanide produces structural chromosome changes in root tips of Vicia faba was confirmed. Like mustards, diepoxides, and maleic hydrazide, potassium cyanide seems to act on cells at early interphase. A tendency of cyanide breaks to be concentrated in heterochromatic segments of the chromosomes was evident. The production of chromosome aberrations by cyanide proved to be practically unaffected by the temperature during treatment. In agreement with Lilly and Thoday, the effect of potassium cyanide was found to be dependent on oxygen tension during treatment. The effect of potassium cyanide increases with increasing oxygen concentration up to 100 per cent oxygen. In the absence of oxygen, potassium cyanide was not completely inactive, but produced a low, though significant frequency of aberrations. Pretreatments with 2.4-dinitrophenol did not influence the effect of potassium cyanide. When bean roots were treated with potassium cyanide before a treatment with 8-ethoxycaffeine, or at the same time as they were treated with 8-ethoxycaffeine, the effect of 8-ethoxycaffeine was almost completely suppressed. The effects of a number of other heavy metal complexing agents were also tested. Sodium fluoride, potassium thiocyanate, carbon monoxide, o-phenanthroline, 2.2-bipyridine, and sodium azide were without radiomimetic effect under the conditions employed, and so was a mixture of sodium azide and sodium fluoride. A low, but quite significant, radiomimetic effect was obtained after treatments with sodium diethyldithiocarbamate, cupferron, and 8-hydroxyquinoline. Under anaerobic conditions, the effects of cyanide and cupferron were both quantitatively and qualitatively indistinguishable. Unlike the effect of cyanide, the effect of cupferron was not enhanced by the presence of oxygen. The effects of the same heavy metal complexing agents were tested on the activities of the enzymes catalase and peroxidase. The activities of both of these enzymes were found to be totally inhibited only by potassium cyanide. In the other cases, little correlation was found between ability to inhibit the activities of these enzymes and ability to produce chromosome aberrations. In a number of experiments, hydrogen peroxide was found to be without radiomimetic effect, whether alone or in combination with potassium cyanide. t-Butyl hydroperoxide proved to be active. The effect of t-butyl hydroperoxide was substantially increased by pretreatments with 2.4.-dinitrophenol. The results are discussed, and it is concluded that the observations made do not support the hypothesis that hydrogen peroxide is involved in the production of chromosome aberrations by potassium cyanide. The possibility that organic peroxides are involved cannot be excluded on the bases of the experimental results. As an alternative hypothesis, it is suggested that iron or other heavy metals are present in the chromosomes and that cyanide and other heavy metal complexing agents produce chromosome aberrations by reacting with these metals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALBERT A. Quantitative studies of the avidity of naturally occurring substances for trace metals. III. Pteridines, riboflavin and purines. Biochem J. 1953 Jul;54(4):646–654. doi: 10.1042/bj0540646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ALLFREY V. G., MIRSKY A. E., STERN H. The chemistry of the cell nucleus. Adv Enzymol Relat Subj Biochem. 1955;16:411–500. doi: 10.1002/9780470122617.ch8. [DOI] [PubMed] [Google Scholar]
  3. Albert A., Gledhill W. S. The choice of a chelating agent for inactivating trace metals: I. A survey of commercially available chelating agents. Biochem J. 1947;41(4):529–533. [PMC free article] [PubMed] [Google Scholar]
  4. Altenburg L. S. The Production of Mutations in Drosophila by Tertiary-Butyl Hydroperoxide. Proc Natl Acad Sci U S A. 1954 Nov;40(11):1037–1040. doi: 10.1073/pnas.40.11.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DARLINGTON C. D., McLENH J. Action of maleic hydrazide on the cell. Nature. 1951 Mar 10;167(4245):407–408. doi: 10.1038/167407a0. [DOI] [PubMed] [Google Scholar]
  6. DICKEY F. H., CLELAND G. H., LOTZ C. The role of organic peroxides in the induction of mutations. Proc Natl Acad Sci U S A. 1949 Oct;35(10):581–586. doi: 10.1073/pnas.35.10.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FEINSTEIN R. N. Perborate as substrate in a new assay of catalase. J Biol Chem. 1949 Oct;180(3):1197–1202. [PubMed] [Google Scholar]
  8. KIHLMAN B. A., MERZ T., SWANSON C. P. Experimentally induced chromosome abberrations in plants. II. The effect of cyanide and other heavy metal complexing agents on the production of chromosome aberrations by x-rays. J Biophys Biochem Cytol. 1957 May 25;3(3):381–390. doi: 10.1083/jcb.3.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KIHLMAN B. Chromosome breakage in Allium by 8-ethoxycaffeine and x-rays. Exp Cell Res. 1955 Apr;8(2):345–368. doi: 10.1016/0014-4827(55)90146-4. [DOI] [PubMed] [Google Scholar]
  10. KIHLMAN B. Effect of oxygen on the frequency of chromosome aberrations produced by 8-ethoxycaffeine. Nature. 1954 Sep 18;174(4429):561–562. doi: 10.1038/174561a0. [DOI] [PubMed] [Google Scholar]
  11. KIHLMAN B. Studies on the effect of oxygen on chromosome breakage induced by 8-ethoxycaffeine. Exp Cell Res. 1955 Apr;8(2):404–407. doi: 10.1016/0014-4827(55)90152-x. [DOI] [PubMed] [Google Scholar]
  12. KIMBALL R. F., HEARON J. Z., GAITHER N. Tests for a role of H2O2 in x-ray mutagenesis. II. Attempts to induce mutations by peroxide. Radiat Res. 1955 Dec;3(4):435–443. [PubMed] [Google Scholar]
  13. KIMBALL R. F. The role of oxygen and peroxide in the production of radiation damage in Paramecium. Ann N Y Acad Sci. 1955 Feb 3;59(4):638–648. doi: 10.1111/j.1749-6632.1955.tb45975.x. [DOI] [PubMed] [Google Scholar]
  14. LOVELESS A. Qualitative aspects of the chemistry and biology of radiomimetic (mutagenic) substances. Nature. 1951 Mar 3;167(4244):338–342. doi: 10.1038/167338a0. [DOI] [PubMed] [Google Scholar]
  15. Mazia D. THE PARTICULATE ORGANIZATION OF THE CHROMOSOME. Proc Natl Acad Sci U S A. 1954 Jun;40(6):521–527. doi: 10.1073/pnas.40.6.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mikaelsen K. PROTECTIVE PROPERTIES OF CYSTEINE, SODIUM HYPOSULFITE, AND SODIUM CYANIDE AGAINST RADIATION INDUCED CHROMOSOME ABERRATIONS. Proc Natl Acad Sci U S A. 1954 Mar;40(3):171–178. doi: 10.1073/pnas.40.3.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SOBELS F. H. The effect of pretreatment with cyanide and azide on the rate of x-ray induced mutations in Drosophila. Z Indukt Abstamm Vererbungsl. 1955;86(4):399–401. doi: 10.1007/BF00317448. [DOI] [PubMed] [Google Scholar]
  18. THODAY J. M., READ J. Effect of oxygen on the frequency of chromosome aberrations produced by alpha-rays. Nature. 1949 Jan 22;163(4134):133–133. doi: 10.1038/163133a0. [DOI] [PubMed] [Google Scholar]
  19. THODAY J. M. The effect of ionizing radiations on the broad bean root. Br J Radiol. 1951 Nov;24(287):622–628. doi: 10.1259/0007-1285-24-287-622. [DOI] [PubMed] [Google Scholar]
  20. Wagner R P, Haddox C H, Fuerst R, Stone W S. The Effect of Irradiated Medium, Cyanide and Peroxide on the Mutation Rate in Neurospora. Genetics. 1950 Mar;35(2):237–248. doi: 10.1093/genetics/35.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wyss O., Clark J. B., Haas F., Stone W. S. The Role of Peroxide in the Biological Effects of Irradiated Broth. J Bacteriol. 1948 Jul;56(1):51–57. [PMC free article] [PubMed] [Google Scholar]

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