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. 1957 May 25;3(3):349–362. doi: 10.1083/jcb.3.3.349

CELLULAR DIFFERENTIATION IN THE KIDNEYS OF NEWBORN MICE STUDIED WITH THE ELECTRON MICROSCOPE

Sam L Clark Jr 1
PMCID: PMC2224034  PMID: 13438920

Abstract

The structure of the kidney of the Swiss albino mouse changes progressively during the first 2 weeks after birth. Cells proliferate to form new nephrons, cells differentiate by acquiring specialized membranous components, and certain cytological features which are present at birth diminish in abundance or disappear. The differentiation of the cells of the cortical tubules has been studied using the light and electron microscopes. The tubules are partially and variably differentiated at birth. During the first 2 weeks after birth the brush border develops in the proximal tubules by the accumulation of numerous microvilli on the apical cell margins. Basal striations develop in proximal and distal tubules as an alignment of mitochondria, the result of what appears to be progressive interlocking of adjacent fluted cells. The mitochondria increase in number and size, accumulate homogeneous matrix, and acquire small, very dense granules. The collecting ducts develop tight pleating of the basal cell membranes, and dark cells containing numerous small cytoplasmic vesicles and microvilli appear. At birth there are dense irregular cytoplasmic inclusions presumed to be lipide in renal cells, the cytoplasmic granules of Palade are abundant, and there are large round bodies in the cells of the proximal tubules. The lipide inclusions disappear a few days after birth, and the cytoplasmic granules of Palade diminish in abundance as the cells differentiate. The large round bodies in the proximal tubules consist of an amorphous material and contain concentrically lamellar structures and mitochondria. They resemble the cytoplasmic droplets produced in the proximal tubules of adult rats and mice by the administration of proteins. The large round bodies disappear from the proximal tubules of infant mice during the first week after birth, but the concentric lamellar structures may be found in adult mice.

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Selected References

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  1. BARTLEY W., DAVIES R. E. Active transport of ions by sub-cellular particles. Biochem J. 1954 May;57(1):37–49. doi: 10.1042/bj0570037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baxter J. S., Yoffey J. M. The post-natal development of renal tubules in the rat. J Anat. 1948 Oct;82(Pt 4):189–197. [PMC free article] [PubMed] [Google Scholar]
  3. DALTON A. J., FELIX M. D. Studies on the Golgi substance of the epithelial cells of the epididymis and duodenum of the mouse. Am J Anat. 1953 Mar;92(2):277–305. doi: 10.1002/aja.1000920204. [DOI] [PubMed] [Google Scholar]
  4. DALTON A. J. Structural details of some of the epithelial cell types in the kidney of the mouse as revealed by the electron microscope. J Natl Cancer Inst. 1951 Jun;11(6):1163–1185. [PubMed] [Google Scholar]
  5. DAVIES J. Cytological evidence of protein absorption in fetal and adult mammalian kidneys. Am J Anat. 1954 Jan;94(1):45–71. doi: 10.1002/aja.1000940103. [DOI] [PubMed] [Google Scholar]
  6. DE ROBERTIS E., FRANCHI C. M. Electron microscope observations on synaptic vesicles in synapses of the retinal rods and cones. J Biophys Biochem Cytol. 1956 May 25;2(3):307–318. doi: 10.1083/jcb.2.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DEMPSEY E. W. Electron microscopy of the visceral yolk-sac epithelium of the guinea pig. Am J Anat. 1953 Nov;93(3):331–363. doi: 10.1002/aja.1000930303. [DOI] [PubMed] [Google Scholar]
  8. DEMPSEY E. W., LANSING A. I. Improved knife-holders for thin-sectioning with rotary microtomes. Proc Soc Exp Biol Med. 1953 Feb;82(2):253–256. doi: 10.3181/00379727-82-20082. [DOI] [PubMed] [Google Scholar]
  9. DEMPSEY E. W., WISLOCKI G. B. The use of silver nitrate as a vital stain, and its distribution in several mammalian tissues as studied with the electron microscope. J Biophys Biochem Cytol. 1955 Mar;1(2):111–118. doi: 10.1083/jcb.1.2.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FALK G. Maturation of renal function in infant rats. Am J Physiol. 1955 Apr;181(1):157–170. doi: 10.1152/ajplegacy.1955.181.1.157. [DOI] [PubMed] [Google Scholar]
  11. KANDEL A., PETERS L., RENNICK B. R. Inhibition of the renal tubular excretion of tetraethylammonium and N'-methylnicotinamide by basic cyanine dyes. J Pharmacol Exp Ther. 1956 Oct;118(2):204–219. [PubMed] [Google Scholar]
  12. LUSE S. A. Electron microscopic observations of the central nervous system. J Biophys Biochem Cytol. 1956 Sep 25;2(5):531–542. doi: 10.1083/jcb.2.5.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OLIVECRONA H., HILLARP N. A. Studies on the submicroscopical structure of the epithelial cells of the intestine, pancreas and kidney in rats during histogenesis. Acta Anat (Basel) 1949;8(3):281–285. doi: 10.1159/000140415. [DOI] [PubMed] [Google Scholar]
  14. PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PALADE G. E., PORTER K. R. Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954 Dec 1;100(6):641–656. doi: 10.1084/jem.100.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PALADE G. E., SIEKEVITZ P. Liver microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Mar 25;2(2):171–200. doi: 10.1083/jcb.2.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PEASE D. C. Electron microscopy of the tubular cells of the kidney cortex. Anat Rec. 1955 Apr;121(4):723–743. doi: 10.1002/ar.1091210403. [DOI] [PubMed] [Google Scholar]
  18. PEASE D. C. Electron microscopy of the vascular bed of the kidney cortex. Anat Rec. 1955 Apr;121(4):701–721. doi: 10.1002/ar.1091210402. [DOI] [PubMed] [Google Scholar]
  19. PEASE D. C. Fine structures of the kidney seen by electron microscopy. J Histochem Cytochem. 1955 Jul;3(4):295–308. doi: 10.1177/3.4.295. [DOI] [PubMed] [Google Scholar]
  20. PORTER K. R., BLUM J. A study in microtomy for electron microscopy. Anat Rec. 1953 Dec;117(4):685–710. doi: 10.1002/ar.1091170403. [DOI] [PubMed] [Google Scholar]
  21. PORTER K. R. Observations on a submicroscopic basophilic component of cytoplasm. J Exp Med. 1953 May;97(5):727–750. doi: 10.1084/jem.97.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SHELDON H., ZETTERQVIST H. An electron microscope study of the corneal epithelium in the vitamin A deficient mouse. Bull Johns Hopkins Hosp. 1956 May;98(5):372–405. [PubMed] [Google Scholar]
  23. SJOSTRAND F. S., ANDERSSON E. Electron microscopy of the intercalated discs of cardiac muscle tissue. Experientia. 1954 Sep 15;10(9):369–370. doi: 10.1007/BF02160542. [DOI] [PubMed] [Google Scholar]
  24. WEISS J. M. Mitochondrial changes induced by potassium and sodium in the duodenal absorptive cell as studied with the electron microscope. J Exp Med. 1955 Dec 1;102(6):783–788. doi: 10.1084/jem.102.6.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WEISS J. M. The ergastoplasm; its fine structure and relation to protein synthesis as studied with the electron microscope in the pancreas of the Swiss albino mouse. J Exp Med. 1953 Dec;98(6):607–618. doi: 10.1084/jem.98.6.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WEISS J. M. The role of the Golgi complex in fat absorption as studied with the electron microscope with observations on the cytology of duodenal absorptive cells. J Exp Med. 1955 Dec 1;102(6):775–782. doi: 10.1084/jem.102.6.775. [DOI] [PMC free article] [PubMed] [Google Scholar]

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