Abstract
1. A method of mass tissue culture has been devised by which, in a relatively short period of time, samples large enough for chemical isolation of mucopolysaccharides can be obtained. 2. Chemical isolation of acid mucopolysaccharides from mass cultures of human fetal skin, human fetal bone, bovine fetal skin, and rat subcutaneous tissue has been carried out. It has been found that the fibroblasts of each of these tissues produce in tissue culture more than one mucopolysaccharide, namely, hyaluronic acid, and a chondroitin sulfate. 3. The chondroitin sulfate produced by fibroblasts of the above tissues in tissue culture was not fully sulfated. The possible significance of this finding is discussed.
Full Text
The Full Text of this article is available as a PDF (339.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DAVIDSON E. A., MEYER K. Chondroitin, a new mucopolysaccharide. J Biol Chem. 1954 Dec;211(2):605–611. [PubMed] [Google Scholar]
- DAVIDSON E., HOFFMAN P., LINKER A., MEYER K. The acid mucopolysaccharides of connective tissue. Biochim Biophys Acta. 1956 Sep;21(3):506–518. doi: 10.1016/0006-3002(56)90188-3. [DOI] [PubMed] [Google Scholar]
- GROSSFELD H. A simplified tissue culture technic. Proc Soc Exp Biol Med. 1949 Jul;71(3):475–475. doi: 10.3181/00379727-71-17229. [DOI] [PubMed] [Google Scholar]
- GROSSFELD H., MEYER K., GODMAN G. Differentiation of fibroblasts in tissue culture, as determined by mucopolysaccharide production. Proc Soc Exp Biol Med. 1955 Jan;88(1):31–35. doi: 10.3181/00379727-88-21484. [DOI] [PubMed] [Google Scholar]
- HOFFMAN P., MEYER K., LINKER A. Transglycosylation during the mixed digestion of hyaluronic acid and chondroitin sulfate by testicular hyaluronidase. J Biol Chem. 1956 Apr;219(2):653–663. [PubMed] [Google Scholar]
- LAYTON L. L. Effect of cortisone upon chondroitin sulfate synthesis by animal tissues. Proc Soc Exp Biol Med. 1951 Mar;76(3):596–598. doi: 10.3181/00379727-76-18571. [DOI] [PubMed] [Google Scholar]
- STOFFYN P. J., JEANLOZ R. W. Identification of amino sugars by paper chromatography. Arch Biochem Biophys. 1954 Oct;52(2):373–379. doi: 10.1016/0003-9861(54)90137-x. [DOI] [PubMed] [Google Scholar]