Abstract
The question of RNA synthesis in enucleate cytoplasm of Amoeba has been approached experimentally by incubating enucleate amoebae in a labelled RNA precursor and determining the incorporation into RNA autoradiographically. The results indicate that there is a cytoplasmic incorporation mechanism which can operate in the absence of the nucleus. A comparison is made between Acetabularia and Amoeba with respect to the origins of cytoplasmic RNA. It is concluded that the existing data are consistent with the assumption that some cytoplasmic RNA is of nuclear origin in both organisms.
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Selected References
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- LINET N., BRACHET J. L'évolution de l'acide ribonucléique et du glycogène dans des fragments nuclés et énuclés d'amibes. Biochim Biophys Acta. 1951 Nov;7(4):607–608. doi: 10.1016/0006-3002(51)90079-0. [DOI] [PubMed] [Google Scholar]
- PLAUT W., RUSTAD R. C. Uptake of adenine-8-14C by whole and fractional amoebae. Nature. 1956 Jan 14;177(4498):89–90. doi: 10.1038/177089a0. [DOI] [PubMed] [Google Scholar]
- PRESCOTT D. M., JAMES T. W. Culturing of Amoeba proteus on Tetrahymena. Exp Cell Res. 1955 Feb;8(1):256–258. doi: 10.1016/0014-4827(55)90067-7. [DOI] [PubMed] [Google Scholar]
- PRESCOTT D. M. The nucleus and ribonucleic acid synthesis in Amoeba. Exp Cell Res. 1957 Feb;12(1):196–198. doi: 10.1016/0014-4827(57)90311-7. [DOI] [PubMed] [Google Scholar]
- RABINOVITCH M., PLAUT W. Cytochemical and autoradiographic observations on nuclear ribonucleic acid in Amoeba proteus. Exp Cell Res. 1956 Feb;10(1):120–124. doi: 10.1016/0014-4827(56)90077-5. [DOI] [PubMed] [Google Scholar]
- RUTMAN R. J., CANTAROW A., PASCHKIS K. E. The catabolism of uracil in vivo and in vitro. J Biol Chem. 1954 Sep;210(1):321–329. [PubMed] [Google Scholar]