Abstract
Quantitative studies of collagen formation by chick embryonic lung tissue grown in media deficient in, or completely lacking, ascorbic acid have been carried out. Cell growth and collagen formation in such cultures can proceed almost normally in media lacking ascorbic acid. Ascorbic acid in combination with whole embryo extract, dialyzed media, or synthetic mixture number 703 was found to have no appreciable effect on cell growth or total collagen formation. This is in marked contrast to the almost total failure of collagen formation in scorbutic animals and suggests that for slow collagen biosynthesis as distinct from more prolific collagen-producing systems, ascorbic acid plays an indirect role.
Full Text
The Full Text of this article is available as a PDF (607.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANERJEE S., BELAVADY B., MUKHERJEE A. K. Effect of dehydroascorbic acid in rabbits. Proc Soc Exp Biol Med. 1953 May;83(1):133–135. doi: 10.3181/00379727-83-20287. [DOI] [PubMed] [Google Scholar]
- BARSKI G., MAURIN J., WIELGOSZ G., LEPINE P. Conditions de nutrition cellulaire in vitro en culture sans support plasmatique; role des fractions micro-et macromoléculaires. Ann Inst Pasteur (Paris) 1951 Jul;81(1):9–32. [PubMed] [Google Scholar]
- FRANCIS M. D., WINNICK T. Studies on the pathway of protein synthesis in tissue culture. J Biol Chem. 1953 May;202(1):273–289. [PubMed] [Google Scholar]
- GERARDE H. W., JONES M. The effect of cortisone on collagen synthesis in vitro. J Biol Chem. 1953 Apr;201(2):553–560. [PubMed] [Google Scholar]
- GERARDE H. W., JONES M., WINNICK T. Protein synthesis and amino acid turnover in tissue culture. J Biol Chem. 1952 May;196(1):51–68. [PubMed] [Google Scholar]
- HEALY G. M., FISHER D. C., PARKER R. C. Nutrition of animal cells in tissue culture. IX. Synthetic medium No. 703. Can J Biochem Physiol. 1954 May;32(3):327–337. [PubMed] [Google Scholar]
- Hass G., McDonald F. Studies of collagen: I. The production of collagen in vitro under variable experimental conditions. Am J Pathol. 1940 Sep;16(5):525–548. [PMC free article] [PubMed] [Google Scholar]
- MARTIN C. J., AXELROD A. E. A modified method for determination of hydroxyproline. Proc Soc Exp Biol Med. 1953 Jul;83(3):461–462. doi: 10.3181/00379727-83-20386. [DOI] [PubMed] [Google Scholar]
- NEUMAN R. E., LOGAN M. A. The determination of collagen and elastin in tissues. J Biol Chem. 1950 Oct;186(2):549–556. [PubMed] [Google Scholar]
- SANFORD K. K., WALTZ H. K., SHANNON J. E., Jr, EARLE W. R., EVANS V. J. The effects of ultrafiltrates and residues of horse serum and chick-embryo extract on proliferation of cells in vitro. J Natl Cancer Inst. 1952 Aug;13(1):121–137. [PubMed] [Google Scholar]
- SCHAFFERT R. R., KINGSLEY G. R. A rapid, simple method for the determination of reduced, dehydro-, and total ascorbic acid in biological material. J Biol Chem. 1955 Jan;212(1):59–68. [PubMed] [Google Scholar]
- VAN ROBERTSON W. B., SCHWARTZ B. Ascorbic acid and the formation of collagen. J Biol Chem. 1953 Apr;201(2):689–696. [PubMed] [Google Scholar]