Abstract
Paramyosin fibrils from the adductor muscles of Venus mercenaria are soluble above neutrality at relatively high ionic strength. From this viscous solution it is possible, by reduction in ionic strength, to reprecipitate acicular crystals of paramyosin. In the electron microscope these fibrils manifest a symmetrical band pattern similar to that previously described by Hodge but differing in some details. The axial periods observed under the conditions of the experiment varied between 1700 and 2000 A and a simple band pattern of one-fifth the main period was frequently observed. ATPase activity of the myosin type but of much lower intensity was demonstrated. Tryptic fission of the protein occurs but the characteristics differ from those of myosin.
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Selected References
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- BEAR R. S., SELBY C. C. The structure of paramyosin fibrils according to x-ray diffraction. J Biophys Biochem Cytol. 1956 Jan 25;2(1):55–69. doi: 10.1083/jcb.2.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOODWIN T. W., JONES O. T. Studies on the biosynthesis of riboflavin. 3. The utilization of 14C-labelled serine for riboflavin biosynthesis by Eremothecium ashbyii. Biochem J. 1956 Sep;64(1):9–13. doi: 10.1042/bj0640009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALL C. E. Method for the observation of macromolecules with the electron microscope illustrated with micrographs of DNA. J Biophys Biochem Cytol. 1956 Sep 25;2(5):625–628. doi: 10.1083/jcb.2.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hodge A. J. A New type of Periodic Structure Obtained by Reconstitution of Paramyosin from Acid Solutions. Proc Natl Acad Sci U S A. 1952 Oct;38(10):850–855. doi: 10.1073/pnas.38.10.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACFARLANE M. G. Clostridium oedematiens n-antigen, an enzyme decomposing tropomyosin. Biochem J. 1955 Oct;61(2):308–315. doi: 10.1042/bj0610308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SZENT-GYORGYI A. G. Meromyosins, the subunits of myosin. Arch Biochem Biophys. 1953 Feb;42(2):305–320. doi: 10.1016/0003-9861(53)90360-9. [DOI] [PubMed] [Google Scholar]