Abstract
Addition of ATP, CaCl2, and KCl to supernatants prepared from mycelia of Snowflake (strain 507), a morphological mutant of Neurospora crassa, results in the formation of filaments 70 nm in diameter. The "decorated" appearance of these filaments after incubation with heavy meromyosin from rabbits suggests they are actin-like.
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- Adelman M. R. Physarum action. Observations on its presence, stability, and assembly in plasmodial extracts and development of an improved purification procedure. Biochemistry. 1977 Nov 1;16(22):4862–4871. doi: 10.1021/bi00641a018. [DOI] [PubMed] [Google Scholar]
- Bettex-Galland M., Probst E., Behnke O. Complex formation with heavy meromyosin of the isolated actin-like component of thrombosthenin, the contractile protein from blood platelets. J Mol Biol. 1972 Jul 28;68(3):533–535. doi: 10.1016/0022-2836(72)90105-2. [DOI] [PubMed] [Google Scholar]
- Clarke M., Schatten G., Mazia D., Spudich J. A. Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 May;72(5):1758–1762. doi: 10.1073/pnas.72.5.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clarke M., Spudich J. A. Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu Rev Biochem. 1977;46:797–822. doi: 10.1146/annurev.bi.46.070177.004053. [DOI] [PubMed] [Google Scholar]
- Comly L. T. Microfilaments in Chaos carolinensis. Membrane association, distribution, and heavy meromyosin binding in the glycerinated cell. J Cell Biol. 1973 Jul;58(1):230–237. doi: 10.1083/jcb.58.1.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishikawa H., Bischoff R., Holtzer H. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969 Nov;43(2):312–328. [PMC free article] [PubMed] [Google Scholar]
- KIELLEY W. W., BRADLEY L. B. The relationship between sulfhydryl groups and the activation of myosin adenosinetriphosphatase. J Biol Chem. 1956 Feb;218(2):653–659. [PubMed] [Google Scholar]
- Nachmias V. T., Huxley H. E. Electron microscope observations on actomyosin and actin preparations from Physarum polycephalum, and on their interaction with heavy meromyosin subfragment I from muscle myosin. J Mol Biol. 1970 May 28;50(1):83–90. doi: 10.1016/0022-2836(70)90105-1. [DOI] [PubMed] [Google Scholar]
- Pollard T. D. Cytoskeletal functions of cytoplasmic contractile proteins. J Supramol Struct. 1976;5(3):317–334. doi: 10.1002/jss.400050306. [DOI] [PubMed] [Google Scholar]
- Pollard T. D., Korn E. D. Electron microscopic identification of actin associated with isolated amoeba plasma membranes. J Biol Chem. 1973 Jan 25;248(2):448–450. [PubMed] [Google Scholar]
- Pollard T. D., Korn E. D. Filaments of Amoeba proteus. II. Binding of heavy meromyosin by thin filaments in motile cytoplasmic extracts. J Cell Biol. 1971 Jan;48(1):216–219. doi: 10.1083/jcb.48.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pollard T. D., Shelton E., Weihing R. R., Korn E. D. Ultrastructural characterization of F-actin isolated from Acanthamoeba castellanii and identification of cytoplasmic filaments as F-actin by reaction with rabbit heavy meromyosin. J Mol Biol. 1970 May 28;50(1):91–97. doi: 10.1016/0022-2836(70)90106-3. [DOI] [PubMed] [Google Scholar]
- Woolley D. E. An actin-like protein from amoebae of dictyostelium discoideum. Arch Biochem Biophys. 1972 Jun;150(2):519–530. doi: 10.1016/0003-9861(72)90070-7. [DOI] [PubMed] [Google Scholar]
- Zucker-Franklin D., Grusky G. The actin and myosin filaments of human and bovine blood platelets. J Clin Invest. 1972 Feb;51(2):419–430. doi: 10.1172/JCI106828. [DOI] [PMC free article] [PubMed] [Google Scholar]