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. 1958 Nov 25;4(6):693–700. doi: 10.1083/jcb.4.6.693

The Ultrastructure of Mouse Lung: The Alveolar Macrophage

H E Karrer 1
PMCID: PMC2224512  PMID: 13610931

Abstract

Free alveolar macrophages of normal mouse lung have been studied in the electron microscope. The tissue was obtained from several young adult white mice. One other animal was instilled intranasally with diluted India ink 1½ hours prior to the removal of the lung. Thin sections of the osmium-fixed, methacrylate-embedded tissue were examined either in an RCA EMU 2 electron microscope or in a Siemens and Halske Elmiskop I b. A few thick sections obtained from the same embeddings were stained for iron. The normal alveolar macrophages, which are usually in contact with the alveolar epithelium, were found to contain a variety of inclusion bodies, along with the usual cytoplasmic components like mitochondria, endoplasmic reticulum, and Palade granules. Another typical component of the cytoplasm of these cells which appears as small (∼6 mµ) very dense granules of composite fine structure is interpreted as ferritin. It is assumed that this ferritin is formed from red blood cells ingested by the alveolar macrophages. The macrophages in the alveoli were found to phagocytize intranasally instilled India ink particles. Such cells, with engulfed India ink particles, were often of more rounded form and the particles were frequently seen lying inside membrane-bound vacuoles or vesicles of the cytoplasm. The membrane of a few vesicles containing India ink particles was seen as the invaginated portion of the cell plasma membrane, and in one instance these same vesicles were seemingly interconnected with a rough surfaced cisterna of the endoplasmic reticulum. The process of phagocytosis is recognized as related to the "normal" process of pinocytosis.

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Selected References

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  1. BARGMANN W., KNOOP A. Vergleichende elektronenmikroskopische Untersuchungen der Lungenkapillaren. Z Zellforsch Mikrosk Anat. 1956;44(3):263–281. [PubMed] [Google Scholar]
  2. BESSIS M. C., BRETON-GORIUS J. Iron particles in normal erythroblasts and normal and pathological erythrocytes. J Biophys Biochem Cytol. 1957 May 25;3(3):503–504. doi: 10.1083/jcb.3.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BESSIS M., BRETON-GORIUS J. Accumulation de granules ferrugineux dans les mitochondries des érythroblastes. C R Hebd Seances Acad Sci. 1957 Jun 3;244(23):2846–2847. [PubMed] [Google Scholar]
  4. BESSIS M., BRETON-GORIUS J. Etude au microscope électronique des granulations ferrugineuses des érythrocytes normaux et pathologiques; anémies hémolytiques; hémoglobinopathies; saturnisme. Rev Hematol. 1957 Jan-Mar;12(1):43–63. [PubMed] [Google Scholar]
  5. BESSIS M., BRETON-GORIUS J. Trois aspects du fer dans des coupes d'organes examinées au microscope électronique (ferritine et dérivé, dans les cellules intestinales, les érythroblastes et les cellules réticulaires). C R Hebd Seances Acad Sci. 1957 Oct 7;245(15):1271–1272. [PubMed] [Google Scholar]
  6. BORYSKO E., BANG F. B. The fine structure of the chorio-allantoic membrane of the normal chick embryo. Bull Johns Hopkins Hosp. 1953 Apr;92(4):257–289. [PubMed] [Google Scholar]
  7. DALTON A. J., FELIX M. D. A comparison of mesothelial cells and macrophages in mice after the intraperitoneal inoculation of melanin granules. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):109–114. doi: 10.1083/jcb.2.4.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EHRET C. F., MINICK O. T., POWERS E. L., ROTH L. E. The internal organization of mitochondria. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):341–346. doi: 10.1083/jcb.2.4.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FARRANT J. L. An electron microscopic study of ferritin. Biochim Biophys Acta. 1954 Apr;13(4):569–576. doi: 10.1016/0006-3002(54)90376-5. [DOI] [PubMed] [Google Scholar]
  10. FAWCETT D. W. Observations on the cytology and electron microscopy of hepatic cells. J Natl Cancer Inst. 1955 Apr;15(5 Suppl):1475–1503. [PubMed] [Google Scholar]
  11. GEY G. O. Some aspects of the constitution and behavior of normal and malignant cells maintained in continuous culture. Harvey Lect. 1954;50:154–229. [PubMed] [Google Scholar]
  12. HARFORD C. G., HAMLIN A., PARKER E. Electron microscopy of HeLa cells after the ingestion of colloidal gold. J Biophys Biochem Cytol. 1957 Sep 25;3(5):749–756. doi: 10.1083/jcb.3.5.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KARRER H. E. An electronmicroscopic study of the fine structure of pulmonary capillaries and alveoli of the mouse; preliminary report. Bull Johns Hopkins Hosp. 1956 Feb;98(2):65–83. [PubMed] [Google Scholar]
  14. KARRER H. E. The ultrastructure of mouse lung fine structure of the capillary endothelium. Exp Cell Res. 1956 Dec;11(3):542–547. doi: 10.1016/0014-4827(56)90163-x. [DOI] [PubMed] [Google Scholar]
  15. KARRER H. E. The ultrastructure of mouse lung; general architecture of capillary and alveolar walls. J Biophys Biochem Cytol. 1956 May 25;2(3):241–252. doi: 10.1083/jcb.2.3.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KARRER H. E. The ultrastructure of mouse lung; some remarks regarding the fine structure of the alveolar basement membrane. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):287–292. doi: 10.1083/jcb.2.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KISCH B. Electron microscopic investigations of the lungs. II. Specific cells and other cells with osmiophylic enclosures. Exp Med Surg. 1957;15(2-3):101–118. [PubMed] [Google Scholar]
  18. LOW F. N. Electron microscopy of the rat lung. Anat Rec. 1952 Aug;113(4):437–449. doi: 10.1002/ar.1091130406. [DOI] [PubMed] [Google Scholar]
  19. LOW F. N. The pulmonary alveolar epithelium of laboratory mammals and man. Anat Rec. 1953 Oct;117(2):241–263. doi: 10.1002/ar.1091170208. [DOI] [PubMed] [Google Scholar]
  20. MOORE D. H., GRIMLEY P. H. Problems in methacrylate embedding for electron microscopy. J Biophys Biochem Cytol. 1957 Mar 25;3(2):255–260. doi: 10.1083/jcb.3.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  24. PALADE G. E. The fine structure of mitochondria. Anat Rec. 1952 Nov;114(3):427–451. doi: 10.1002/ar.1091140304. [DOI] [PubMed] [Google Scholar]
  25. POLICARD A., COLLET A., GILTAIRE-RALYTE L. Etude an microscope électronique des cellules alvéolaires du poumon normal de mammifère. C R Hebd Seances Acad Sci. 1955 Jun 20;240(25):2363–2365. [PubMed] [Google Scholar]
  26. POLICARD A., COLLET A., PREGERMAIN S. Etude au microscope électronique du granulome pulmonaire silicotique expérimental. Presse Med. 1957 Jan 23;65(7):121–124. [PubMed] [Google Scholar]
  27. PORTER K. R., BLUM J. A study in microtomy for electron microscopy. Anat Rec. 1953 Dec;117(4):685–710. doi: 10.1002/ar.1091170403. [DOI] [PubMed] [Google Scholar]
  28. RICHTER G. W. Electron microscopy of hemosiderin; presence of ferritin and occurrence of crystalline lattices in hemosiderin deposits. J Biophys Biochem Cytol. 1958 Jan 25;4(1):55–58. doi: 10.1083/jcb.4.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. ROTH L. E. An electron microscope study of the cytology of the protozoan Euplotes patella. J Biophys Biochem Cytol. 1957 Nov 25;3(6):985–1000. doi: 10.1083/jcb.3.6.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SEDAR A. W., PORTER K. R. The fine structure of cortical components of Paramecium multimicronucleatum. J Biophys Biochem Cytol. 1955 Nov 25;1(6):583–604. doi: 10.1083/jcb.1.6.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. SJOSTRAND F. S., HANZON V. Membrane structures of cytoplasm and mitochondria in exocrine cells of mouse pancreas as revealed by high resolution electron microscopy. Exp Cell Res. 1954 Nov;7(2):393–414. doi: 10.1016/s0014-4827(54)80086-3. [DOI] [PubMed] [Google Scholar]

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