Skip to main content
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727

Staining of Tissue Sections for Electron Microscopy with Heavy Metals

II. Application of Solutions Containing Lead and Barium

Michael L Watson 1
PMCID: PMC2224513  PMID: 13610936

Abstract

Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.

Full Text

The Full Text of this article is available as a PDF (794.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHARD W., ROUILLER C. Microbodies and the problem of mitochondrial regeneration in liver cells. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):355–360. doi: 10.1083/jcb.2.4.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DE HARVEN E., FRIEND C. Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report. J Biophys Biochem Cytol. 1958 Mar 25;4(2):151–156. doi: 10.1083/jcb.4.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. SATIR P. G., PEACHEY L. D. Thin sections. II. A simple method for reducing compression artifacts. J Biophys Biochem Cytol. 1958 May 25;4(3):345–348. doi: 10.1083/jcb.4.3.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. WATSON M. L. Reduction of heating artifacts in thin sections examined in the electron microscope. J Biophys Biochem Cytol. 1957 Nov 25;3(6):1017–1022. doi: 10.1083/jcb.3.6.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES