Abstract
A new technique for the cultivation of living tissues in the multipurpose culture chamber is described. This procedure employs strips of cellophane as the agent for anchoring tissue explants to the coverslip walls of the chamber and disposes of the time-honored plasma-clot technique. The primary advance embodied in this procedure lies in the fact that cells emigrating from so-cultured explants manifest themselves in a highly differentiated manner comparable to the cells of origin, whereas the outgrowth from the same types of tissue in plasma clots results in a more undifferentiated type of growth. Comparisons of outgrowths from embryonic thyroid, bone, and muscle (chicken) are photographically documented, and attention is called to certain cytochemical methods which further corroborate the differentiated quality obtained with the cellophane-strip technique.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fell H. B. The Osteogenic Capacity in vitro of Periosteum and Endosteum Isolated from the Limb Skeleton of Fowl Embryos and Young Chicks. J Anat. 1932 Jan;66(Pt 2):157–180.11. [PMC free article] [PubMed] [Google Scholar]
- HEALY G. M., FISHER D. C., PARKER R. C. Nutrition of animal cells in tissue culture. X. Synthetic medium No. 858. Proc Soc Exp Biol Med. 1955 May;89(1):71–77. doi: 10.3181/00379727-89-21719. [DOI] [PubMed] [Google Scholar]
- POMERAT C. M. Tissue culture in experimental biology and medicine. J Am Vet Med Assoc. 1956 Nov 15;129(10):458–462. [PubMed] [Google Scholar]
- ROSE G. G. An appraisal of a biological response by strain HeLa cells in tissue culture to 100 human sera. Cancer Res. 1958 May;18(4):411–417. [PubMed] [Google Scholar]
- ROSE G. G. Microkinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques. J Biophys Biochem Cytol. 1957 Sep 25;3(5):697–704. doi: 10.1083/jcb.3.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSE G. G. Special uses of the multipurpose tissue culture chamber. Tex Rep Biol Med. 1957;15(2):310–312. [PubMed] [Google Scholar]
- ROSE G. A separable and multipurpose tissue culture chamber. Tex Rep Biol Med. 1954;12(4):1074–1083. [PubMed] [Google Scholar]
- WOODLIFF H. J. A further use of Rose's tissue culture chamber. Tex Rep Biol Med. 1958;16(3):380–384. [PubMed] [Google Scholar]