Abstract
The human epidermis has been studied by electron microscopy following osmium tetroxide and potassium permanganate fixation. An anatomically distinct cell in the human epidermis has been demonstrated with features similar to the melanocyte of the hair bulb described by Barnicot, Birbeck and Cuckow (3). It is dendritic in form and does not contain tonofilaments. "Intercellular bridges" are not formed. The mitochondria are larger and more numerous than those of other epidermal cells and the endoplasmic reticulum is more complex. Some of these cells contain melanin but others are melanin-free. The cell has been interpreted as being identical with the dopa-positive, clear cell of Masson (dendritic cell of Bloch or melanocyte). We have found that many membranous structures in the human epidermis are better preserved by permanganate fixation than by osmium tetroxide fixation.
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Selected References
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- BILLINGHAM R. E. Dendritic cells in pigmented human skin. J Anat. 1949 Apr;83(2):109–115. [PubMed] [Google Scholar]
- BIRBECK M. S., CUCKOW F. W. The electron microscopy of human hair pigments. Ann Hum Genet. 1955 Feb;19(3):231–249. doi: 10.1111/j.1469-1809.1955.tb01346.x. [DOI] [PubMed] [Google Scholar]
- BIRBECK M. S., MERCER E. H., BARNICOT N. A. The structure and formation of pigment granules in human hair. Exp Cell Res. 1956 Apr;10(2):505–514. doi: 10.1016/0014-4827(56)90022-2. [DOI] [PubMed] [Google Scholar]
- BIRBECK M. S., MERCER E. H. The electron microscopy of the human hair follicle. I. Introduction and the hair cortex. J Biophys Biochem Cytol. 1957 Mar 25;3(2):203–214. doi: 10.1083/jcb.3.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHARLES A., SMIDDY F. G. The tonofibrils of the human epidermis. J Invest Dermatol. 1957 Nov;29(5):327–338. doi: 10.1038/jid.1957.108. [DOI] [PubMed] [Google Scholar]
- FAWCETT D. W., SELBY C. C. Observations on the fine structure of the turtle atrium. J Biophys Biochem Cytol. 1958 Jan 25;4(1):63–72. doi: 10.1083/jcb.4.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIROUD A., LEBLOND C. P. The keratinization of epidermis and its derivatives, especially the hair, as shown by x-ray diffraction and histochemical studies. Ann N Y Acad Sci. 1951 Mar;53(3):613–626. doi: 10.1111/j.1749-6632.1951.tb31963.x. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELBY C. C. An electron microscope study of the epidermis of mammalian skin in thin sections. I. Dermo-epidermal junction and basal cell layer. J Biophys Biochem Cytol. 1955 Sep 25;1(5):429–444. doi: 10.1083/jcb.1.5.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELBY C. C. An electron microscope study of thin sections of human skin. II. Superficial cell layers of footpad epidermis. J Invest Dermatol. 1957 Aug;29(2):131–149. doi: 10.1038/jid.1957.80. [DOI] [PubMed] [Google Scholar]
- WEISS P., FERRIS W. Electronmicrograms of larval amphibian epidermis. Exp Cell Res. 1954 May;6(2):546–549. doi: 10.1016/0014-4827(54)90210-4. [DOI] [PubMed] [Google Scholar]