Abstract
In the eggs of four species of echinoderms, Mespilia globulus, Pseudocentrotus depressus, Hemicentrotus pulcherrimus and Clypeaster japonicus, changes in the distribution of protein-bound SH groups from fertilization to the 2 cell stage have been studied cytochemically by use of a mercaptide-forming azo dye. In the eggs of these species, the color intensity in the cytoplasm increased upon fertilization. The astral centers and spindle during mitosis were stained deeply. When the aster formation was suppressed by ether, hyaline spots appeared in the egg cytoplasm instead of well formed astral centers and these spots were stained by the SH-specific dye. Upon recovery of such eggs in pure sea water, and when cleavage ensued, such spots disappeared and two new astral centers were reorganized. The SH-protein occurring in the centrosphere is considered to be the precursor material for the asters and spindle, and this material is apparently derived from the cytoplasm.
Full Text
The Full Text of this article is available as a PDF (750.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARRNETT R. J., SELIGMAN A. M. Histochemical demonstration of protein-bound sulfhydryl groups. Science. 1952 Sep 26;116(3013):323–327. doi: 10.1126/science.116.3013.323. [DOI] [PubMed] [Google Scholar]
- BENNETT H. S. The demonstration of thiol groups in certain tissues by means of a new colored sulfhydryl reagent. Anat Rec. 1951 Jun;110(2):231–246. doi: 10.1002/ar.1091100208. [DOI] [PubMed] [Google Scholar]
- MESCON H., FLESCH P. Modification of Bennett's method for the histochemical demonstration of free sulfhydryl groups in skin. J Invest Dermatol. 1952 Mar;18(3):261–266. doi: 10.1038/jid.1952.29. [DOI] [PubMed] [Google Scholar]
- Mazia D., Dan K. The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells. Proc Natl Acad Sci U S A. 1952 Sep;38(9):826–838. doi: 10.1073/pnas.38.9.826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SWANN M. M. The mechanism of cell division: experiments with ether on the sea urchin egg. Exp Cell Res. 1954 Nov;7(2):505–517. doi: 10.1016/s0014-4827(54)80094-2. [DOI] [PubMed] [Google Scholar]