Abstract
The two distinct types of cytoplasm seen with the light microscope in the adipose cell of the leech Glossiphonia complanata have been identified in the electron microscope image of this cell. One of these, the basophil cytoplasm, contains many well oriented, paired membranes which are much more clearly evident when calcium ions are added to the fixative. The membranes sometimes appear as concentric arrays of lamellae and are thought to represent sections through a phospholipide-containing body. The paired membranes and the concentric lamellae have granules attached to them and resemble in size and structure the membranes of the endoplasmic reticulum encountered in many mammalian cells. Small dense cytoplasmic particles are present throughout the cell; they may be ferritin molecules, derived from the breakdown of haemoglobin taken in as food. On the basis of a previous histochemical study and the present electron microscope investigation, it is suggested that these paired membranes are similar to the organized type of mammalian ER and the results seem to confirm the belief that these membranes are composed of layers of phospholipoprotein together with attached particles of ribonucleoprotein.
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Selected References
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- BRADBURY S., CLAYTON B. P. Occurrence of masked lipid in mouse pancreas. Nature. 1958 May 10;181(4619):1347–1347. doi: 10.1038/1811347a0. [DOI] [PubMed] [Google Scholar]
- CAULFIELD J. B. Effects of varying the vehicle for OsO4 in tissue fixation. J Biophys Biochem Cytol. 1957 Sep 25;3(5):827–830. doi: 10.1083/jcb.3.5.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E., PORTER K. R. Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954 Dec 1;100(6):641–656. doi: 10.1084/jem.100.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E., SIEKEVITZ P. Pancreatic microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Nov 25;2(6):671–690. doi: 10.1083/jcb.2.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POLICARD A., BESSIS M., BRETONGORIUS J. Structures myéliniques observées au microscope électronique sur des coupes de globules rouges en voie de lyse. Exp Cell Res. 1957 Aug;13(1):184–186. doi: 10.1016/0014-4827(57)90066-6. [DOI] [PubMed] [Google Scholar]
- SJOSTRAND F. S., HANZON V. Membrane structures of cytoplasm and mitochondria in exocrine cells of mouse pancreas as revealed by high resolution electron microscopy. Exp Cell Res. 1954 Nov;7(2):393–414. doi: 10.1016/s0014-4827(54)80086-3. [DOI] [PubMed] [Google Scholar]
- SMITH J. T., FUNCKES A. J., BARAK A. J., THOMAS L. E. Cellular lipoproteins. I. The insoluble lipoprotein of whole liver cells. Exp Cell Res. 1957 Aug;13(1):96–102. doi: 10.1016/0014-4827(57)90048-4. [DOI] [PubMed] [Google Scholar]
- STOECKENIUS W. OsO4-Fixierung intrazellulärer Myelinfiguren. Exp Cell Res. 1957 Oct;13(2):410–414. doi: 10.1016/0014-4827(57)90024-1. [DOI] [PubMed] [Google Scholar]