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. 1959 Jan 25;5(1):93–95. doi: 10.1083/jcb.5.1.93

Production of the Milk Agent in Cultures of Mouse Mammary Carcinoma

E Y Lasfargues 1, Dan H Moore 1, Margaret R Murray 1, Cushman D Haagensen 1, E C Pollard 1
PMCID: PMC2224638  PMID: 13630939

Abstract

Thin sections of tissue cultures grown from tumors of the RIII high-breast-cancer strain mice were studied in the electron microscope. These tissues contain an abundance of particles whose morphology is consistent with biophysical measurement of the milk agent. These particles, found only extracellularly in our cultures, are formed at the cell membrane. The process of formation, as reconstructed from sections, appears to include a thickening and protrusion of the cell membrane which then evolves gradually into a dense sphere and separates from the cell in much the same manner as does influenza virus. The contents of the newly formed body are later rearranged to form a nucleoid within a membranous sac.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANG F. B., ANDERVONT H. B., VELLISTO I. Electron microscopic evidence concerning the mammary tumor inciter (virus). II. An electron microscopic study of spontaneous and induced mammary tumors of mice. Bull Johns Hopkins Hosp. 1956 Apr;98(4):287–307. [PubMed] [Google Scholar]
  2. BANG F. B., VELLISTO I., LIBERT R. Electron microscopic evidence concerning the mammary tumor inciter (virus). I. A study of normal and malignant cells from the mammary gland of mice. Bull Johns Hopkins Hosp. 1956 Apr;98(4):255–285. [PubMed] [Google Scholar]
  3. BENEDETTI E. L., BERNHARD W. Recherches ultrastructurales sur le virus de la leucémie érythroblastique du poulet. J Ultrastruct Res. 1958 Aug;1(4):309–336. doi: 10.1016/s0022-5320(58)90004-2. [DOI] [PubMed] [Google Scholar]
  4. BERNHARD W., BAUER A., GUERIN M., OBERLING C. Etude au microscope électronique de corpuscules d'aspect virusal dans des épithéliomas mammaires de la souris. Bull Assoc Fr Etud Cancer. 1955;42(2):163–178. [PubMed] [Google Scholar]
  5. BERNHARD W. Electron microscopy of tumor cells and tumor viruses; a review. Cancer Res. 1958 Jun;18(5):491–509. [PubMed] [Google Scholar]
  6. DMOCHOWSKI L., HAAGENSEN C. D., MOORE D. H. Studies of sections of normal and malignant cells of high- and low-cancer-strain mice by means of electron microscope. Acta Unio Int Contra Cancrum. 1955;11(6):640–645. [PubMed] [Google Scholar]
  7. HARFORD C. G., HAMLIN A., PARKER E. Electron microscopy of early cytoplasmic changes due to influenza virus. J Exp Med. 1955 Jun 1;101(6):577–590. doi: 10.1084/jem.101.6.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LASFARGUES E. Y., MOORE D. H., MURRAY M. R. Maintenance of the milk factor in cultures of mouse mammary epithelium. Cancer Res. 1958 Dec;18(11):1281–1285. [PubMed] [Google Scholar]
  9. MOORE D. H., GRIMLEY P. H. Problems in methacrylate embedding for electron microscopy. J Biophys Biochem Cytol. 1957 Mar 25;3(2):255–260. doi: 10.1083/jcb.3.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MORGAN C., ROSE H. M., MOORE D. H. Structure and development of viruses observed in the electron microscope. III. Influenza virus. J Exp Med. 1956 Aug 1;104(2):171–182. doi: 10.1084/jem.104.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PALADE G. E., SIEKEVITZ P. Liver microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Mar 25;2(2):171–200. doi: 10.1083/jcb.2.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PORTER K. R., BLUM J. A study in microtomy for electron microscopy. Anat Rec. 1953 Dec;117(4):685–710. doi: 10.1002/ar.1091170403. [DOI] [PubMed] [Google Scholar]
  14. SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. II. Functional variations in the enzymatic activity of microsomes. J Biophys Biochem Cytol. 1958 May 25;4(3):309–318. doi: 10.1083/jcb.4.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]

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