Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Sep;135(3):920–927. doi: 10.1128/jb.135.3.920-927.1978

Oxygen and nitrate in utilization by Bacillus licheniformis of the arginase and arginine deiminase routes of arginine catabolism and other factors affecting their syntheses.

K Broman, N Lauwers, V Stalon, J M Wiame
PMCID: PMC222465  PMID: 690081

Abstract

Bacillus licheniformis has two pathways of arginine catabolism. In well-aerated cultures, the arginase route is present, and levels of catabolic ornithine carbamoyltransferase were low. An arginase pathway-deficient mutant, BL196, failed to grow on arginine as a nitrogen source under these conditions. In anaerobiosis, the wild type contained very low levels of arginase and ornithine transaminase. BL196 grew normally on glucose plus arginine in anaerobiosis and, like the wild type, had appreciable levels of catabolic transferase. Nitrate, like oxygen, repressed ornithine carbamoyltransferase and stimulated arginase synthesis. In aerobic cultures, arginase was repressed by glutamine in the presence of glucose, but not when the carbon-energy source was poor. In anaerobic cultures, ammonia repressed catabolic ornithine carbamoyltransferase, but glutamate and glutamine stimulated its synthesis. A second mutant, derived from BL196, retained the low arginase and ornithine transaminase levels of BL196 but produced high levels of deiminase pathway enzymes in the presence of oxygen.

Full text

PDF
920

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAUCHOP T., ELSDEN S. R. The growth of micro-organisms in relation to their energy supply. J Gen Microbiol. 1960 Dec;23:457–469. doi: 10.1099/00221287-23-3-457. [DOI] [PubMed] [Google Scholar]
  2. Broman K., Stalon V., Wiame J. M. The duplication of arginine catabolism and the meaning of the two ornithine carbamoyltransferases in Bacillus licheniformis. Biochem Biophys Res Commun. 1975 Sep 16;66(2):821–827. doi: 10.1016/0006-291x(75)90583-5. [DOI] [PubMed] [Google Scholar]
  3. Dean D. R., Hoch J. A., Aronson A. I. Alteration of the Bacillus subtilis glutamine synthetase results in overproduction of the enzyme. J Bacteriol. 1977 Sep;131(3):981–987. doi: 10.1128/jb.131.3.981-987.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fisher S. H., Sonenshein A. L. Glutamine-requiring mutants of Bacillus subtilis. Biochem Biophys Res Commun. 1977 Dec 7;79(3):987–995. doi: 10.1016/0006-291x(77)91207-4. [DOI] [PubMed] [Google Scholar]
  5. Gancedo C., Holzer H. Enzymatic inactivation of glutamine synthetase in Enterobacteriaceae. Eur J Biochem. 1968 Apr 3;4(2):190–192. doi: 10.1111/j.1432-1033.1968.tb00192.x. [DOI] [PubMed] [Google Scholar]
  6. Halleux P., Legrain C., Stalon V., Piérard A., Wiame J. M. Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A study of the quaternary structure. Eur J Biochem. 1972 Dec 4;31(2):386–393. doi: 10.1111/j.1432-1033.1972.tb02545.x. [DOI] [PubMed] [Google Scholar]
  7. Harwood C. R., Baumberg S. Arginine hydroxamate-resistant mutants of Bacillus subtilis with altered control of arginine metabolism. J Gen Microbiol. 1977 May;100(1):177–188. doi: 10.1099/00221287-100-1-177. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Laishley E. J., Bernlohr R. W. Regulation of arginine and proline catabolism in Bacillus licheniformis. J Bacteriol. 1968 Aug;96(2):322–329. doi: 10.1128/jb.96.2.322-329.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laishley E. J., Bernlohr R. W. The regulation and kinetics of the two ornithine transcarbamylase enzymes of Bacillus licheniformis. Biochim Biophys Acta. 1968 Nov 19;167(3):547–554. doi: 10.1016/0005-2744(68)90044-2. [DOI] [PubMed] [Google Scholar]
  11. Lemille F., de Barjac H., Bonnefoi A. Essai sur la classification biochimique de 97 Bacillus du groupe I, appartenant à 9 espèces différentes. Ann Inst Pasteur (Paris) 1969 Jun;116(6):808–819. [PubMed] [Google Scholar]
  12. Magasanik B., Prival M. J., Brenchley J. E., Tyler B. M., DeLeo A. B., Streicher S. L., Bender R. A., Paris C. G. Glutamine synthetase as a regulator of enzyme synthesis. Curr Top Cell Regul. 1974;8(0):119–138. doi: 10.1016/b978-0-12-152808-9.50010-9. [DOI] [PubMed] [Google Scholar]
  13. RAMALEY R. F., BERNLOHR R. W. APPARENT INDUCTION OF ORNITHINE TRANSCARBAMYLASE AND ARGINASE BY ARGININE IN BACILLUS LICHENIFORMIS. J Mol Biol. 1965 Apr;11:842–844. doi: 10.1016/s0022-2836(65)80041-9. [DOI] [PubMed] [Google Scholar]
  14. Ramos F., Thuriaux P., Wiame J. M., Bechet J. The participation of ornithine and citrulline in the regulation of arginine metabolism in Saccharomyces cerevisiae. Eur J Biochem. 1970 Jan;12(1):40–47. doi: 10.1111/j.1432-1033.1970.tb00818.x. [DOI] [PubMed] [Google Scholar]
  15. Reysset G., Aubert J. P. Relationship between sporulation and mutations impairing glutamine synthetase in Bacillus megaterium. Biochem Biophys Res Commun. 1975 Aug 18;65(4):1237–1241. doi: 10.1016/s0006-291x(75)80362-7. [DOI] [PubMed] [Google Scholar]
  16. Schulp J. A., Stouthamer A. H. The influence of oxygen, glucose and nitrate upon the formation of nitrate reductase and the respiratory system in Bacillus licheniformis. J Gen Microbiol. 1970 Dec;64(2):195–203. doi: 10.1099/00221287-64-2-195. [DOI] [PubMed] [Google Scholar]
  17. Stalon V., Ramos F., Piérard A., Wiame J. M. Regulation of the catabolic ornithine carbamoyltransferase of Pseudomonas fluorescens. A comparison with the anabolic transferase and with a mutationally modified catabolic transferase. Eur J Biochem. 1972 Aug 18;29(1):25–35. doi: 10.1111/j.1432-1033.1972.tb01953.x. [DOI] [PubMed] [Google Scholar]
  18. Stalon V., Ramos F., Piérard A., Wiame J. M. The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas. Biochim Biophys Acta. 1967 May 16;139(1):91–97. doi: 10.1016/0005-2744(67)90115-5. [DOI] [PubMed] [Google Scholar]
  19. WIAME J. M., STORCK R. Métabolisme de l'acide glutamique chez Bacillus subtilis. Biochim Biophys Acta. 1953 Feb;10(2):268–279. doi: 10.1016/0006-3002(53)90250-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES