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. 1959 Dec 1;6(3):423–426. doi: 10.1083/jcb.6.3.423

Electron Microscopy of Neurospora crassa Mycelia

A J Shatkin 1, E L Tatum 1
PMCID: PMC2224685  PMID: 14445619

Abstract

The fine structure of vegetative mycelia of the filamentous Ascomycete, Neurospora crassa, has been investigated by the standard techniques of electron microscopy. Addition of uranyl nitrate to the methacrylate-embedding medium minimized disruption of the specimens—an accident often observed in the preparation of microbial material. This report describes the presence of a chitinous polysaccharide wall containing fine fibrils embedded in a homogeneous matrix. A sinuate plasma membrane lies adjacent to the inner wall surface. This membrane is often closely associated with the endoplasmic reticulum of the cytoplasm. Numerous mitochondria of the classical type, and dense particles of 10 mµ diameter occur throughout the cytoplasm. The nuclear region is surrounded by a double membrane with pore openings. Associated with the nuclear envelope is a dense area, the nucleolus. The significance of these observations and their relationship to other forms is discussed.

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Selected References

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  1. AGAR H. D., DOUGLAS H. C. Studies on the cytological structure of yeast: electron microscopy of thin sections. J Bacteriol. 1957 Mar;73(3):365–375. doi: 10.1128/jb.73.3.365-375.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLUMENTHAL H. J., ROSEMAN S. Quantitative estimation of chitin in fungi. J Bacteriol. 1957 Aug;74(2):222–224. doi: 10.1128/jb.74.2.222-224.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BORYSKO E., SAPRANAUSKAS P. A new technique for comparative phase-contrast and electron microscope studies of cells grown in tissue culture, with an evaluation of the technique by means of time-lapse cinemicrographs. Bull Johns Hopkins Hosp. 1954 Aug;95(2):68–79. [PubMed] [Google Scholar]
  4. Barratt R. W., Garnjobst L. Genetics of a Colonial Microconidiating Mutant Strain of Neurospora Crassa. Genetics. 1949 Jul;34(4):351–369. doi: 10.1093/genetics/34.4.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GIRBARDT M. Uber die Substruktur von Polystictus versicolor L. Arch Mikrobiol. 1958;28(3):255–269. [PubMed] [Google Scholar]
  6. GLAUERT A. M., GLAUERT R. H., ROGERS G. E. A new embedding medium for electron microscopy. Nature. 1956 Oct 13;178(4537):803–803. doi: 10.1038/178803a0. [DOI] [PubMed] [Google Scholar]
  7. HASHIMOTO T., CONTI S. F., NAYLOR H. B. Fine structure of microorganisms. III. Electron microscopy of resting and germinating ascospores of Saccharomyces cerevisiae. J Bacteriol. 1958 Oct;76(4):406–416. doi: 10.1128/jb.76.4.406-416.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HASKINS F. A., TISSIERES A., MITCHELL H. K., MITCHELL M. B. Cytochromes and the succinic acid oxidase system of poky strains of Neurospora. J Biol Chem. 1953 Feb;200(2):819–826. [PubMed] [Google Scholar]
  9. HENRY B. S., OHERN E. M. A cytological study of Coccidioides immitis by electron microscopy. J Bacteriol. 1956 Nov;72(5):632–645. doi: 10.1128/jb.72.5.632-645.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLENBERGER E., TURIAN G. Ultrastructure du corps paranucléaire, des mitochondries et de la membrane nucléaire des gamètes d'Allomyces macrogynus. Exp Cell Res. 1956 Aug;11(2):417–422. doi: 10.1016/0014-4827(56)90117-3. [DOI] [PubMed] [Google Scholar]
  11. MOORE D. H., GRIMLEY P. H. Problems in methacrylate embedding for electron microscopy. J Biophys Biochem Cytol. 1957 Mar 25;3(2):255–260. doi: 10.1083/jcb.3.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McALEAR J. H., EDWARDS G. A. [Continuity of plasma membrane and nuclear membrane]. Exp Cell Res. 1959 Mar;16(3):689–692. doi: 10.1016/0014-4827(59)90139-9. [DOI] [PubMed] [Google Scholar]
  13. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  15. PALADE G. E. The fine structure of mitochondria. Anat Rec. 1952 Nov;114(3):427–451. doi: 10.1002/ar.1091140304. [DOI] [PubMed] [Google Scholar]
  16. RYTER A., KELLENBERGER E. L'inclusion au polyester pour l'ultramicrotomie. J Ultrastruct Res. 1958 Dec;2(2):200–214. doi: 10.1016/s0022-5320(58)90018-2. [DOI] [PubMed] [Google Scholar]
  17. Tatum E. L., Barratt R. W., Cutter V. M., Jr Chemical Induction of Colonial Paramorphs in Neurospora and Syncephalastrum. Science. 1949 May 20;109(2838):509–511. doi: 10.1126/science.109.2838.509. [DOI] [PubMed] [Google Scholar]
  18. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WHALEY W. G., MOLLENHAUER H. H., KEPHART J. E. The endoplasmic reticulum and the Golgi structures in maize root cells. J Biophys Biochem Cytol. 1959 May 25;5(3):501–506. doi: 10.1083/jcb.5.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]

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