Abstract
The primary purpose of the experiments reported in this paper was to gain information on the molecular origin of the mitotic apparatus. Antisera were prepared against unfertilized sea urchin (Strongylocentrotus purpuratus) egg antigens and mitotic apparatus antigens. These were permitted to react with various antigen solutions in Ouchterlony agar gel diffusion plates, and the resultant precipitation patterns analysed. The results revealed that the mitotic apparatus contains probably no more than two antigens (precursor-1 component and precursor-2 component) and that these are shared by the unfertilized egg. Absorption and fractionation techniques indicated that in the unfertilized egg the precursor-1 component is present both as a "soluble" protein and as an insoluble form tenaciously associated with intracellular structural elements. A survey of dividing and non-dividing tissues for the precursor-1 component revealed that it was restricted to tissues in which mitotic activity could be detected microscopically. No immunochemical relationship could be detected between the mitotic apparatus and proteins extracted, by various methods, from the lantern muscle.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANDERSON N. G. Cell division. I. A theoretical approach to the primeval mechanism, the initiation of cell division, and chromosomal condensation. Q Rev Biol. 1956 Sep;31(3):169–199. doi: 10.1086/401393. [DOI] [PubMed] [Google Scholar]
- HOLTZER H., ABBOTT J., CAVANAUGH M. W. Some properties of embryonic cardiac myoolasts. Exp Cell Res. 1959 Mar;16(3):595–601. doi: 10.1016/0014-4827(59)90127-2. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mazia D., Dan K. The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells. Proc Natl Acad Sci U S A. 1952 Sep;38(9):826–838. doi: 10.1073/pnas.38.9.826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand. 1949;26(4):507–515. doi: 10.1111/j.1699-0463.1949.tb00751.x. [DOI] [PubMed] [Google Scholar]
- OUDIN J. B. Specific precipitation in gels and its application to immunochemical analysis. Methods Med Res. 1952;5:335–378. [PubMed] [Google Scholar]
- RUSTAD R. C. An interference microscopical and cytochemical analysis of local mass changes in the mitotic apparatus during mitosis. Exp Cell Res. 1959 Mar;16(3):575–583. doi: 10.1016/0014-4827(59)90125-9. [DOI] [PubMed] [Google Scholar]
- SWANN M. M. The control of cell division: a review. II. Special mechanisms. Cancer Res. 1958 Nov;18(10):1118–1160. [PubMed] [Google Scholar]
- WENT H. A. A micro homogenizer for small-volume samples. Biochim Biophys Acta. 1958 Jan;27(1):165–167. doi: 10.1016/0006-3002(58)90304-4. [DOI] [PubMed] [Google Scholar]
- WENT H. A. Some immunochemical studies on the mitotic apparatus of the sea urchin. J Biophys Biochem Cytol. 1959 Mar 25;5(2):353–356. doi: 10.1083/jcb.5.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]